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Conference Paper: Effects of lipopolysaccharide on cell cycling of human PDL cells
| Title | Effects of lipopolysaccharide on cell cycling of human PDL cells |
|---|---|
| Authors | |
| Keywords | Cell culture Inflammatory mediators and Periodontics |
| Issue Date | 2013 |
| Publisher | International Association for Dental Research - Asia Pacific Region (IADR-APR). |
| Citation | The 2nd Meeting of International Association for Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013, p. abstract no. 645 How to Cite? |
| Abstract | Objective: To investigate the effects of porphyromonas gingivalis-lipopolysaccharide (Pg-LPS) on the cell cycling of the human periodontal ligament cells ( hPDLCs) in vitro.
Method: HPDLCs (3rd to 5th passages) were incubated with 0.01ng/ml Pg-LPS for 24 hours in vitro, and then the DNA synthesis in different phases of cell cycling of the hPDLCs were analyzed using the flow cytometry (FCM).
Result: It was found that the G0/G1-phase fraction of hPDLCs was decreased significantly by Pg-LPS (p<0.01), while the proliferation index (PI) was increased (p<0.05 ). Both the S-phase fraction (SPF) and the G2/M-phase fraction were higher in hPDLCs incubated with Pg-LPS than those in control cells (p<0.05 for SPF; p<0.01 for G2/M-phase fraction).
Conclusion: Pg- LPS with the assessed concentration enhances the proliferation of hPDLCs by augmenting the DNA synthesis in both S-phase and G2/M phase of cell cycling.
This abstract is based on research that was funded entirely or partially by an outside source: Small Project Funding, HKU (201109176111) |
| Description | Conference theme: We are the future Poster Session Fulltext in: https://iadr.confex.com/iadr/apr13/webprogram/Paper181769.html |
| Persistent Identifier | http://hdl.handle.net/10722/186546 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Lu, J | en_US |
| dc.contributor.author | Yang, Y | en_US |
| dc.contributor.author | Zhang, C | en_US |
| dc.date.accessioned | 2013-08-20T12:12:19Z | - |
| dc.date.available | 2013-08-20T12:12:19Z | - |
| dc.date.issued | 2013 | en_US |
| dc.identifier.citation | The 2nd Meeting of International Association for Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013, p. abstract no. 645 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10722/186546 | - |
| dc.description | Conference theme: We are the future | - |
| dc.description | Poster Session | - |
| dc.description | Fulltext in: https://iadr.confex.com/iadr/apr13/webprogram/Paper181769.html | - |
| dc.description.abstract | Objective: To investigate the effects of porphyromonas gingivalis-lipopolysaccharide (Pg-LPS) on the cell cycling of the human periodontal ligament cells ( hPDLCs) in vitro. Method: HPDLCs (3rd to 5th passages) were incubated with 0.01ng/ml Pg-LPS for 24 hours in vitro, and then the DNA synthesis in different phases of cell cycling of the hPDLCs were analyzed using the flow cytometry (FCM). Result: It was found that the G0/G1-phase fraction of hPDLCs was decreased significantly by Pg-LPS (p<0.01), while the proliferation index (PI) was increased (p<0.05 ). Both the S-phase fraction (SPF) and the G2/M-phase fraction were higher in hPDLCs incubated with Pg-LPS than those in control cells (p<0.05 for SPF; p<0.01 for G2/M-phase fraction). Conclusion: Pg- LPS with the assessed concentration enhances the proliferation of hPDLCs by augmenting the DNA synthesis in both S-phase and G2/M phase of cell cycling. This abstract is based on research that was funded entirely or partially by an outside source: Small Project Funding, HKU (201109176111) | - |
| dc.language | eng | en_US |
| dc.publisher | International Association for Dental Research - Asia Pacific Region (IADR-APR). | - |
| dc.relation.ispartof | Meeting of International Association for Dental Research - Asia Pacific Region | en_US |
| dc.subject | Cell culture | - |
| dc.subject | Inflammatory mediators and Periodontics | - |
| dc.title | Effects of lipopolysaccharide on cell cycling of human PDL cells | en_US |
| dc.type | Conference_Paper | en_US |
| dc.identifier.email | Yang, Y: yangyanq@hkucc.hku.hk | en_US |
| dc.identifier.email | Zhang, C: zhangcf@hku.hk | en_US |
| dc.identifier.authority | Yang, Y=rp00045 | en_US |
| dc.identifier.authority | Zhang, C=rp01408 | en_US |
| dc.identifier.hkuros | 220551 | en_US |
| dc.identifier.spage | abstract no. 645 | - |
| dc.identifier.epage | abstract no. 645 | - |
| dc.publisher.place | Thailand | - |