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- Publisher Website: 10.1016/j.pain.2007.05.023
- Scopus: eid_2-s2.0-40049094003
- PMID: 17689191
- WOS: WOS:000254912700005
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Article: IL-1ra alleviates inflammatory hyperalgesia through preventing phosphorylation of NMDA receptor NR-1 subunit in rats
Title | IL-1ra alleviates inflammatory hyperalgesia through preventing phosphorylation of NMDA receptor NR-1 subunit in rats |
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Authors | |
Keywords | Hyperalgesia IL-1β NMDA receptor Phosphorylation Rat Spinal cord |
Issue Date | 2008 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/pain |
Citation | Pain, 2008, v. 135 n. 3, p. 232-239 How to Cite? |
Abstract | Although it has been shown that pro-inflammatory cytokines such as interleukin-1β (IL-1β) facilitate perception of noxious inputs at the spinal level, the mechanisms have not been understood. This study determined the cell type that produces IL-1β, the co-localization of IL-1 receptor type I (IL-1RI) and Fos and NR1 in the spinal cord, and the effects of IL-1 receptor antagonist (IL-1ra) on NR1 phosphorylation and hyperalgesia in a rat model of inflammatory pain. Phosphorylation of NR1, an essential subunit of the NMDA receptor (NMDAR), is known to modulate NMDAR activity and facilitate pain. Hyperalgesia was induced by injecting complete Freund's adjuvant (CFA, 0.08 ml, 40 μg Mycobacterium tuberculosis) into one hind paw of each rat. Paw withdrawal latency (PWL) was tested before CFA (-48 h) for baseline and 2 and 24 h after CFA to assess hyperalgesia. IL-1ra was given (i.t.) 24 h before CFA to block the action of basal IL-1β and 2 h prior to each of two PWL tests to block CFA-induced IL-1β. Spinal cords were removed for double immunostaining of IL-1β/neuronal marker and IL-1β/glial cell markers, IL-1RI/Fos and IL-1RI/NR1, and for Western blot to measure NR1 phosphorylation. The data showed that: (1) astrocytes produce IL-1β, (2) IL-1RI is localized in Fos- and NR1-immunoreactive neurons within the spinal dorsal horn, and (3) IL-1ra at 0.01 mg/rat significantly increased PWL (P < 0.05) and inhibited NR1 phosphorylation compared to saline control. The results suggest that spinal IL-1β is produced by astrocytes and enhances NR1 phosphorylation to facilitate inflammatory pain. © 2007 International Association for the Study of Pain. |
Persistent Identifier | http://hdl.handle.net/10722/188594 |
ISSN | 2023 Impact Factor: 5.9 2023 SCImago Journal Rankings: 2.376 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, RX | en_US |
dc.contributor.author | Li, A | en_US |
dc.contributor.author | Liu, B | en_US |
dc.contributor.author | Wang, L | en_US |
dc.contributor.author | Ren, K | en_US |
dc.contributor.author | Zhang, H | en_US |
dc.contributor.author | Berman, BM | en_US |
dc.contributor.author | Lao, L | en_US |
dc.date.accessioned | 2013-09-03T04:10:29Z | - |
dc.date.available | 2013-09-03T04:10:29Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Pain, 2008, v. 135 n. 3, p. 232-239 | en_US |
dc.identifier.issn | 0304-3959 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/188594 | - |
dc.description.abstract | Although it has been shown that pro-inflammatory cytokines such as interleukin-1β (IL-1β) facilitate perception of noxious inputs at the spinal level, the mechanisms have not been understood. This study determined the cell type that produces IL-1β, the co-localization of IL-1 receptor type I (IL-1RI) and Fos and NR1 in the spinal cord, and the effects of IL-1 receptor antagonist (IL-1ra) on NR1 phosphorylation and hyperalgesia in a rat model of inflammatory pain. Phosphorylation of NR1, an essential subunit of the NMDA receptor (NMDAR), is known to modulate NMDAR activity and facilitate pain. Hyperalgesia was induced by injecting complete Freund's adjuvant (CFA, 0.08 ml, 40 μg Mycobacterium tuberculosis) into one hind paw of each rat. Paw withdrawal latency (PWL) was tested before CFA (-48 h) for baseline and 2 and 24 h after CFA to assess hyperalgesia. IL-1ra was given (i.t.) 24 h before CFA to block the action of basal IL-1β and 2 h prior to each of two PWL tests to block CFA-induced IL-1β. Spinal cords were removed for double immunostaining of IL-1β/neuronal marker and IL-1β/glial cell markers, IL-1RI/Fos and IL-1RI/NR1, and for Western blot to measure NR1 phosphorylation. The data showed that: (1) astrocytes produce IL-1β, (2) IL-1RI is localized in Fos- and NR1-immunoreactive neurons within the spinal dorsal horn, and (3) IL-1ra at 0.01 mg/rat significantly increased PWL (P < 0.05) and inhibited NR1 phosphorylation compared to saline control. The results suggest that spinal IL-1β is produced by astrocytes and enhances NR1 phosphorylation to facilitate inflammatory pain. © 2007 International Association for the Study of Pain. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/pain | en_US |
dc.relation.ispartof | Pain | en_US |
dc.subject | Hyperalgesia | - |
dc.subject | IL-1β | - |
dc.subject | NMDA receptor | - |
dc.subject | Phosphorylation | - |
dc.subject | Rat | - |
dc.subject | Spinal cord | - |
dc.subject.mesh | Analgesics - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Anti-Inflammatory Agents - Pharmacology | en_US |
dc.subject.mesh | Astrocytes - Drug Effects - Immunology | en_US |
dc.subject.mesh | Disease Models, Animal | en_US |
dc.subject.mesh | Gliosis - Drug Therapy - Immunology - Physiopathology | en_US |
dc.subject.mesh | Hyperalgesia - Drug Therapy - Immunology - Physiopathology | en_US |
dc.subject.mesh | Inflammation - Drug Therapy - Immunology - Physiopathology | en_US |
dc.subject.mesh | Inflammation Mediators - Pharmacology | en_US |
dc.subject.mesh | Interleukin 1 Receptor Antagonist Protein - Pharmacology | en_US |
dc.subject.mesh | Interleukin-1Beta - Antagonists & Inhibitors - Immunology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Pain - Drug Therapy - Immunology - Physiopathology | en_US |
dc.subject.mesh | Pain Threshold - Drug Effects - Physiology | en_US |
dc.subject.mesh | Phosphorylation - Drug Effects | en_US |
dc.subject.mesh | Posterior Horn Cells - Drug Effects - Immunology - Physiopathology | en_US |
dc.subject.mesh | Proto-Oncogene Proteins C-Fos - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Reaction Time - Drug Effects - Immunology | en_US |
dc.subject.mesh | Receptors, Interleukin-1 - Antagonists & Inhibitors - Immunology | en_US |
dc.subject.mesh | Receptors, N-Methyl-D-Aspartate - Drug Effects - Metabolism | en_US |
dc.title | IL-1ra alleviates inflammatory hyperalgesia through preventing phosphorylation of NMDA receptor NR-1 subunit in rats | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lao, L: lxlao1@hku.hk | en_US |
dc.identifier.authority | Lao, L=rp01784 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.pain.2007.05.023 | en_US |
dc.identifier.pmid | 17689191 | - |
dc.identifier.scopus | eid_2-s2.0-40049094003 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-40049094003&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 135 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 232 | en_US |
dc.identifier.epage | 239 | en_US |
dc.identifier.isi | WOS:000254912700005 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Zhang, RX=7404864527 | en_US |
dc.identifier.scopusauthorid | Li, A=16245342100 | en_US |
dc.identifier.scopusauthorid | Liu, B=55720712900 | en_US |
dc.identifier.scopusauthorid | Wang, L=9036448600 | en_US |
dc.identifier.scopusauthorid | Ren, K=7102272533 | en_US |
dc.identifier.scopusauthorid | Zhang, H=51563012400 | en_US |
dc.identifier.scopusauthorid | Berman, BM=35458606800 | en_US |
dc.identifier.scopusauthorid | Lao, L=7005681883 | en_US |
dc.identifier.issnl | 0304-3959 | - |