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Conference Paper: Adenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cells
Title | Adenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cells |
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Authors | |
Issue Date | 2012 |
Publisher | ICOHAD 2012. |
Citation | The 2012 International Congress on Oncogenic Herpesviruses and Associated Diseases (ICOHAD 2012), Philadelphia, PA., 1-4 August 2012. In Conference Program, 2012, p. 38, abstract no. 3.17 How to Cite? |
Abstract | Epstein-Barr virus (EBV) encodes 48 mature microRNAs that play important roles in viral maintenance and promote host cell survival by regulating viral transcripts expression, inhibiting apoptosis or facilitating to evade cell immune surveillance. We have previously shown that EBV-encoded miR-BART5 targets and downregulates cellular pro-apoptotic protein p53-upregulated modulator of apoptosis (PUMA) to promote cellular survival of EBV-infected nasopharyngeal carcinoma (NPC) cells. Since compromising miR-BART5 might induce apoptosis of EBV-infected NPC cell, in this study we have established an adenoviral expression system to deliver anti-miR-BART5 decoys to NPC cells. The anti-miR-BART5 decoys comprised 6 tandem repeats of miR-BART5 binding sites and their expression was driven by EBVEBER2 promoter. They were designed to serve as a competitive inhibitor of miR-BART5 to reverse miR-BART5's inhibitory effects on PUMA in EBV-infected NPC cells. The RNA polymerase III-dependent EBER2 promoter is particularly strong in ... |
Description | Poster Session 1 - Vaccines and Anti-Viral Therapeutics: no. 3.17 |
Persistent Identifier | http://hdl.handle.net/10722/189763 |
DC Field | Value | Language |
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dc.contributor.author | Yuen, KS | en_US |
dc.contributor.author | Tang, VHM | en_US |
dc.contributor.author | Kok, KH | en_US |
dc.contributor.author | Jin, D | en_US |
dc.date.accessioned | 2013-09-17T14:56:08Z | - |
dc.date.available | 2013-09-17T14:56:08Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | The 2012 International Congress on Oncogenic Herpesviruses and Associated Diseases (ICOHAD 2012), Philadelphia, PA., 1-4 August 2012. In Conference Program, 2012, p. 38, abstract no. 3.17 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/189763 | - |
dc.description | Poster Session 1 - Vaccines and Anti-Viral Therapeutics: no. 3.17 | - |
dc.description.abstract | Epstein-Barr virus (EBV) encodes 48 mature microRNAs that play important roles in viral maintenance and promote host cell survival by regulating viral transcripts expression, inhibiting apoptosis or facilitating to evade cell immune surveillance. We have previously shown that EBV-encoded miR-BART5 targets and downregulates cellular pro-apoptotic protein p53-upregulated modulator of apoptosis (PUMA) to promote cellular survival of EBV-infected nasopharyngeal carcinoma (NPC) cells. Since compromising miR-BART5 might induce apoptosis of EBV-infected NPC cell, in this study we have established an adenoviral expression system to deliver anti-miR-BART5 decoys to NPC cells. The anti-miR-BART5 decoys comprised 6 tandem repeats of miR-BART5 binding sites and their expression was driven by EBVEBER2 promoter. They were designed to serve as a competitive inhibitor of miR-BART5 to reverse miR-BART5's inhibitory effects on PUMA in EBV-infected NPC cells. The RNA polymerase III-dependent EBER2 promoter is particularly strong in ... | - |
dc.language | eng | en_US |
dc.publisher | ICOHAD 2012. | - |
dc.relation.ispartof | International Congress on Oncogenic Herpesviruses & Associated Diseases, ICOHAD 2012 | en_US |
dc.title | Adenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cells | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Tang, VHM: tanghmv@hku.hk | en_US |
dc.identifier.email | Kok, KH: khkok@hku.hk | en_US |
dc.identifier.email | Jin, D: dyjin@hku.hk | en_US |
dc.identifier.authority | Kok, KH=rp01455 | en_US |
dc.identifier.authority | Jin, D=rp00452 | en_US |
dc.description.nature | postprint | - |
dc.identifier.hkuros | 224777 | en_US |
dc.identifier.spage | 38, abstract no. 3.17 | - |
dc.identifier.epage | 38, abstract no. 3.17 | - |
dc.publisher.place | United States | - |