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Conference Paper: Oral commensals and pathogens enhance hBD-2 expression in HOKs
Title | Oral commensals and pathogens enhance hBD-2 expression in HOKs |
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Authors | |
Keywords | Host-microbial interactions Immune response Immunology Microbiology and Periodontal disease |
Issue Date | 2013 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 14 How to Cite? |
Abstract | Objectives: Human β-defensins (hBDs) are antimicrobial peptides expressed by epithelial cells and leukocytes that resist the microbial colonization. Notably, hBD-2 has both antibacterial activity and chemoattractant effects, and therefore plays a vital role in innate immune system. The present study investigated hBD-2 expression in a model of human oral keratinocytes (HOKs) in response to oral commensal and periodontopathogenic bacteria. Cell surface receptors involved in the bacteria-host interaction were examined.
Methods: Representative oral commensal bacteria (Streptococcus mitis, Smi and Streptococcus sanguinis, Ss) and pathogenic bacteria (Porphyromonas gingivalis, Pg) were selected. The expression of hBD-2 in the interaction of HOKs with the species was assessed in a bacteria-HOKs co-culture model during a time course of 15 to 120 min, using both ELISA and qPCR. Toll-like receptors (TLR) 2 and 4 were analyzed using qPCR and antibody-mediated blocking assays.
Results: Multiplicity of infection (MOI) 10 was found to be appropriate for the co-culture model. Overall, Smi, Ss and Pg significantly upregulated the expression of hBD-2 in HOKs with reference to the control (p<0.05). As compared to the control Ss and Pg greatly stimulated hBD-2 expression at 30 (p<0.05), 60 (p<0.01) and 120 min (p<0.05). Whereas, the significant upregulation of hBD-2 was only observed at 60 and 120 min in HOKs stimulated by Smi. Blocking assays showed
that Smi- and Ss-stimulated expression of hBD-2 in HOKs was through both TLRs 2 and 4, while blockage of TLRs 2 and 4 did not significantly affect hBD-2 expression.
Conclusion: This study suggests that hBD-2 expression in HOKs may be significantly enhanced by the challenge of Smi, Ss and Pg. TLRs 2 and 4 are involved in the Smi- and Ss-stimulated expression of hBD-2 in HOKs. Supported by the Hong Kong RGC (HKU768411M and HKU767512M) and the Modern Dental Laboratory/HKU Endowment Fund to LJJ. |
Description | Conference Theme: We are the Future Oral Presentation Session 3: O3 |
Persistent Identifier | http://hdl.handle.net/10722/192566 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Li, H | en_US |
dc.contributor.author | Seneviratne, CJ | en_US |
dc.contributor.author | Lau, ASY | en_US |
dc.contributor.author | Wang, CY | en_US |
dc.contributor.author | Jin, L | en_US |
dc.date.accessioned | 2013-11-18T05:06:40Z | - |
dc.date.available | 2013-11-18T05:06:40Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 14 | en_US |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/10722/192566 | - |
dc.description | Conference Theme: We are the Future | - |
dc.description | Oral Presentation | - |
dc.description | Session 3: O3 | - |
dc.description.abstract | Objectives: Human β-defensins (hBDs) are antimicrobial peptides expressed by epithelial cells and leukocytes that resist the microbial colonization. Notably, hBD-2 has both antibacterial activity and chemoattractant effects, and therefore plays a vital role in innate immune system. The present study investigated hBD-2 expression in a model of human oral keratinocytes (HOKs) in response to oral commensal and periodontopathogenic bacteria. Cell surface receptors involved in the bacteria-host interaction were examined. Methods: Representative oral commensal bacteria (Streptococcus mitis, Smi and Streptococcus sanguinis, Ss) and pathogenic bacteria (Porphyromonas gingivalis, Pg) were selected. The expression of hBD-2 in the interaction of HOKs with the species was assessed in a bacteria-HOKs co-culture model during a time course of 15 to 120 min, using both ELISA and qPCR. Toll-like receptors (TLR) 2 and 4 were analyzed using qPCR and antibody-mediated blocking assays. Results: Multiplicity of infection (MOI) 10 was found to be appropriate for the co-culture model. Overall, Smi, Ss and Pg significantly upregulated the expression of hBD-2 in HOKs with reference to the control (p<0.05). As compared to the control Ss and Pg greatly stimulated hBD-2 expression at 30 (p<0.05), 60 (p<0.01) and 120 min (p<0.05). Whereas, the significant upregulation of hBD-2 was only observed at 60 and 120 min in HOKs stimulated by Smi. Blocking assays showed that Smi- and Ss-stimulated expression of hBD-2 in HOKs was through both TLRs 2 and 4, while blockage of TLRs 2 and 4 did not significantly affect hBD-2 expression. Conclusion: This study suggests that hBD-2 expression in HOKs may be significantly enhanced by the challenge of Smi, Ss and Pg. TLRs 2 and 4 are involved in the Smi- and Ss-stimulated expression of hBD-2 in HOKs. Supported by the Hong Kong RGC (HKU768411M and HKU767512M) and the Modern Dental Laboratory/HKU Endowment Fund to LJJ. | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | - |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | - |
dc.subject | Host-microbial interactions | - |
dc.subject | Immune response | - |
dc.subject | Immunology | - |
dc.subject | Microbiology and Periodontal disease | - |
dc.title | Oral commensals and pathogens enhance hBD-2 expression in HOKs | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Seneviratne, CJ: jaya@hku.hk | en_US |
dc.identifier.email | Lau, ASY: asylau@hku.hk | en_US |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | en_US |
dc.identifier.authority | Seneviratne, CJ=rp01372 | en_US |
dc.identifier.authority | Lau, ASY=rp00474 | en_US |
dc.identifier.authority | Jin, L=rp00028 | en_US |
dc.identifier.hkuros | 226796 | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.issue | Special Issue B: abstract no. 14 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |