File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: Effects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells
Title | Effects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells |
---|---|
Authors | |
Keywords | 5F NCI-H460 cells NF-κB Pteris semipinnata L. |
Issue Date | 2010 |
Citation | Chinese Traditional and Herbal Drugs, 2010, v. 41 n. 3, p. 435-439 How to Cite? |
Abstract | Objective: To investigate the inhibitory mechanism of 5F isolated from Pteris semipinnata on the proliferation and apoptosis induction of NCI-H460 cells. The roles of NF-κB pathway in the apoptosis were explored in this study. Methods: NCI-H460 Cell growth inhibition mediated by 5F was detected by MTT assay. RT-PCR was Applied to examing the mRNA levels of IκKβ, IκB, p65, and p50. Results: 5F Showed the growth inhibitory activity against NCI-H460 cells with IC50 values of 21.40, 4.52, and 1.02 μg/mL for 24, 48, and 72 h, respectively. The mRNA expression levels of IκKβ and IκB were significantly decreased after 5F treated cells for 6 h (P<0.05); The mRNA expression levels of p65 and p50 were significantly decreased after 5F treated cells for 3 h (P<0.05). Conclusion: The mechanism for 5F induction of NCI-H460 cells apoptosis could be mediated by the inhibition of NF-κB pathway. |
Persistent Identifier | http://hdl.handle.net/10722/192677 |
ISSN | 2023 SCImago Journal Rankings: 0.169 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, Y | en_US |
dc.contributor.author | Chen, GG | en_US |
dc.contributor.author | Lü, Y-N | en_US |
dc.contributor.author | Hsin, MKY | en_US |
dc.contributor.author | Underwood, MJ | en_US |
dc.contributor.author | Liang, N-C | en_US |
dc.date.accessioned | 2013-11-20T04:55:10Z | - |
dc.date.available | 2013-11-20T04:55:10Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | Chinese Traditional and Herbal Drugs, 2010, v. 41 n. 3, p. 435-439 | en_US |
dc.identifier.issn | 0253-2670 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/192677 | - |
dc.description.abstract | Objective: To investigate the inhibitory mechanism of 5F isolated from Pteris semipinnata on the proliferation and apoptosis induction of NCI-H460 cells. The roles of NF-κB pathway in the apoptosis were explored in this study. Methods: NCI-H460 Cell growth inhibition mediated by 5F was detected by MTT assay. RT-PCR was Applied to examing the mRNA levels of IκKβ, IκB, p65, and p50. Results: 5F Showed the growth inhibitory activity against NCI-H460 cells with IC50 values of 21.40, 4.52, and 1.02 μg/mL for 24, 48, and 72 h, respectively. The mRNA expression levels of IκKβ and IκB were significantly decreased after 5F treated cells for 6 h (P<0.05); The mRNA expression levels of p65 and p50 were significantly decreased after 5F treated cells for 3 h (P<0.05). Conclusion: The mechanism for 5F induction of NCI-H460 cells apoptosis could be mediated by the inhibition of NF-κB pathway. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Chinese Traditional and Herbal Drugs | en_US |
dc.subject | 5F | - |
dc.subject | NCI-H460 cells | - |
dc.subject | NF-κB | - |
dc.subject | Pteris semipinnata L. | - |
dc.title | Effects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells | en_US |
dc.type | Article | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-77952717201 | en_US |
dc.identifier.volume | 41 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 435 | en_US |
dc.identifier.epage | 439 | en_US |
dc.identifier.issnl | 0253-2670 | - |