Periodontal and peri-implant microbiota in patients with both healthy and inflamed peri-implant tissues

Abstract

Aim: To compare the prevalence and levels of putative ‘periodontal pathogens’ present in the subgingival/submucosal microbiota surrounding teeth or implants in subjects with differing periodontal/peri-implant health statuses.

Materials and Methods: 22 subjects were included in the study. Within each subject, 4 sites were selected for sampling: (1) healthy implant, (2) diseased implant [PPD≥5mm, presence of bleeding on probing (BOP) and radiographic bone loss], (3) healthy tooth and (4) periodontally-diseased tooth (presence of BOP, PPD≥4mm). Subgingival/submucosal plaque was sampled using paper points. After DNA extraction, quantitative real-time PCR (q-PCR) was used to detect and quantify six bacterial species: Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis and Staphylococcus. aureus. The absolute counts were log10-transformed before statistical analysis.

Results: The most commonly-detected species were F. nucleatum (n=85), T. denticola (n=84) and S. aureus (n=82); while P. gingivalis had the lowest detection frequency (n=61). The overall detection frequencies of target species was generally higher in diseased compared to healthy sites with no statistically significant differences for any particular species (p>0.05, Cochran Q test). Regarding bacterial loads, no significant differences were found among groups except for F. nucleatum (p=0.023, Friedman’s 2-way ANOVA). Submucosal/subgingival plaque from diseased teeth (3.95) and implants (3.54) harbored significantly more F. nucleatum than healthy implants (3.06) and teeth (2.93). Both periodontal and peri-implant sites, independent of health status, were found to harbor S.aureus. The loads (log10) of S.aureus were ca. 3.5 for all the 4 groups (p=0.232), which was the highest of all the species detected.

Conclusions: Our results indicate that putative periodontal pathogens are common to both periodontal and peri-implant sites independent of the health status, which may explain the high risk of peri-implantitis in patients with untreated periodontal diseases. S. aureus is commonly present in the subgingival/submucosal microbiota surrounding both teeth and implants.

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. . . . Oral Presentation Sessions : * If you see any discrepancies of the content, please contact Mr. Alvin Kong for assistance. Venue: Please refer to different oral presentation sessions in the table below.

Oral Presentation Session A

Oral Presentation Session B

Oral Presentation Session C

Oral Presentation Session D

Oral Presentation Session B : Infection and Immunity - Junior and Senior category December 12, 2013 11:00am - 1:00pm Lecture Theatre 2, G/F., The Prince Philip Dental Hiospital.

Presentation Session & Time

Presenter

Oral Presentation Title

Category

1 Sess. B LT 2

11:00am-11:15am Li, Xuan

Baicalin loading efficiencies in spherical and rod-like mesoporous silica nanoparticles synthesized by lipid crystal mechanism

Abstract

Oral presentation - junior 2 Sess. B LT 2

11:15am-11:30am Zhuang, Long-fei

Periodontal and peri-implant microbiota in patients with both healthy and inflamed peri-implant tissues

Abstract

Oral presentation - junior 3 Sess. B LT 2

11:30am-11:45am Li, Peng

Inability to Eradicate Candida Biofilm Persisters by Consecutive Antifungal Treatments

Abstract

Oral presentation - junior 4 Sess. B LT 2

11:45am-12:00pm