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Conference Paper: Reduced level of induced endocytosis in Candida albicans sap7 mutants
Title | Reduced level of induced endocytosis in Candida albicans sap7 mutants |
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Authors | |
Keywords | Genetics Host-microbial interactions Infection Pathogenicity |
Issue Date | 2014 |
Publisher | International Association for Dental Research. The Conference abstracts' website is located at https://iadr.confex.com/iadr/search.epl |
Citation | The 2014 International Association for Dental Research (IADR) - Pan European Regional (PER) Congress, Dubrovnik, Croatia, 10-13 September 2014. How to Cite? |
Abstract | OBJECTIVE: To investigate the effect of C. albicans SAP7 gene in induced endocytosis in Candida-epithelial cell interactions. METHOD: In this study, C. albicans sap7 mutant strain was constructed in our laboratory by a PCR – based gene disruption method. In the endocytosis assay, the number of C. albicans cells that were cell-associated with and endocytosed by OKF6/TERT2 was determined using differential fluorescence assay with minor modifications. Briefly, conflucent OKF6/TERT2 cells were infected with 1×105 Candida cells for 3 h incubation. After removing nonadherent organisms, the cells were fixed with 3% paraformaldehyde. The adherent but not endocytosed ... |
Description | Poster Session: Scientific Groups - 90. Microbiology/Immunology III: no. 655 |
Persistent Identifier | http://hdl.handle.net/10722/198156 |
DC Field | Value | Language |
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dc.contributor.author | Yang, H | - |
dc.contributor.author | Tsang, PCS | - |
dc.contributor.author | Pow, EHN | - |
dc.contributor.author | Tsang, PWK | - |
dc.date.accessioned | 2014-06-25T02:49:36Z | - |
dc.date.available | 2014-06-25T02:49:36Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | The 2014 International Association for Dental Research (IADR) - Pan European Regional (PER) Congress, Dubrovnik, Croatia, 10-13 September 2014. | - |
dc.identifier.uri | http://hdl.handle.net/10722/198156 | - |
dc.description | Poster Session: Scientific Groups - 90. Microbiology/Immunology III: no. 655 | - |
dc.description.abstract | OBJECTIVE: To investigate the effect of C. albicans SAP7 gene in induced endocytosis in Candida-epithelial cell interactions. METHOD: In this study, C. albicans sap7 mutant strain was constructed in our laboratory by a PCR – based gene disruption method. In the endocytosis assay, the number of C. albicans cells that were cell-associated with and endocytosed by OKF6/TERT2 was determined using differential fluorescence assay with minor modifications. Briefly, conflucent OKF6/TERT2 cells were infected with 1×105 Candida cells for 3 h incubation. After removing nonadherent organisms, the cells were fixed with 3% paraformaldehyde. The adherent but not endocytosed ... | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Conference abstracts' website is located at https://iadr.confex.com/iadr/search.epl | - |
dc.relation.ispartof | IADR/PER Congress 2014 | - |
dc.subject | Genetics | - |
dc.subject | Host-microbial interactions | - |
dc.subject | Infection | - |
dc.subject | Pathogenicity | - |
dc.title | Reduced level of induced endocytosis in Candida albicans sap7 mutants | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Tsang, PCS: csptsang@hkucc.hku.hk | - |
dc.identifier.email | Pow, EHN: ehnpow@hku.hk | - |
dc.identifier.email | Tsang, PWK: pwktsang@hku.hk | - |
dc.identifier.authority | Tsang, PCS=rp00026 | - |
dc.identifier.authority | Pow, EHN=rp00030 | - |
dc.identifier.authority | Tsang, PWK=rp01388 | - |
dc.description.nature | postprint | - |
dc.identifier.hkuros | 229297 | - |
dc.publisher.place | United States | - |