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Conference Paper: Development of Novel 3D Stem Cell Sheet for Periodontal Regeneration
Title | Development of Novel 3D Stem Cell Sheet for Periodontal Regeneration |
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Authors | |
Keywords | Cell biology Periodontal disease Regeneration Stem cells and Tissue engineering |
Issue Date | 2014 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1067 How to Cite? |
Abstract | Objective: To assess the regenerative capacity of 3D periodontal ligament stem cell (PDLSC) sheet in vivo.
Method: Single culture and co-culture cell sheets were prepared by using PDLSCs and Human umbilical vein endothelial cells (HUVECs) in thermo-responsive culture dishes. Scanning electron microscopy (SEM) was used to observe the ultra-structure of single and co-culture groups of cell sheets. Cells were labeled with cell-tracker dyes (PDLSCs-Green, HUVECs-Red) and seeded in different ratios to assess the time dependent modulation of vascular network formation on Matrigel (4 and 8 hours). Tooth root fragments were coated with PDLSC sheets and implanted into the subcutaneous space of SCID mice. After 2 weeks, implants were retrieved and processed for histological evaluation.
Result: SEM results showed the ultra-structural arrangement and preserved extracellular matrix (ECM) of layered cell sheet constructs. Furthermore, increased ECM deposition was observed in co-culture groups. PDLSCs and HUVECs seeded on Matrigel showed initiation of vessel like structure formation after 4 hours. Extensive lattice of vessel like structures was formed in 1:2 and 1:5 groups compared to other groups. However, after 8 hours all the groups showed formation of island like cell aggregations. In vivo, H&E sections exemplified the successful integration of implanted PDL cell sheet coated roots with host tissues after 2 weeks. Periodontal ligament-like arrangement was observed around root surface in cell sheet groups compared to control groups.
Conclusions: 3D cell sheet model demonstrated a promising regenerative potential in vivo and this approach would be useful in future periodontal bioengineering strategies. This abstract is based on research that was funded entirely or partially by an outside source: Seed Funding for Basic Research 201111159191 The University of Hong Kong |
Description | Poster Presentation Session 151: Periodontal Tissue Regeneration |
Persistent Identifier | http://hdl.handle.net/10722/199331 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Panduwawala, CP | en_US |
dc.contributor.author | Samaranayake, LP | en_US |
dc.contributor.author | Jin, L | en_US |
dc.contributor.author | Zhang, C | en_US |
dc.date.accessioned | 2014-07-22T01:13:40Z | - |
dc.date.available | 2014-07-22T01:13:40Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1067 | en_US |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/10722/199331 | - |
dc.description | Poster Presentation | - |
dc.description | Session 151: Periodontal Tissue Regeneration | - |
dc.description.abstract | Objective: To assess the regenerative capacity of 3D periodontal ligament stem cell (PDLSC) sheet in vivo. Method: Single culture and co-culture cell sheets were prepared by using PDLSCs and Human umbilical vein endothelial cells (HUVECs) in thermo-responsive culture dishes. Scanning electron microscopy (SEM) was used to observe the ultra-structure of single and co-culture groups of cell sheets. Cells were labeled with cell-tracker dyes (PDLSCs-Green, HUVECs-Red) and seeded in different ratios to assess the time dependent modulation of vascular network formation on Matrigel (4 and 8 hours). Tooth root fragments were coated with PDLSC sheets and implanted into the subcutaneous space of SCID mice. After 2 weeks, implants were retrieved and processed for histological evaluation. Result: SEM results showed the ultra-structural arrangement and preserved extracellular matrix (ECM) of layered cell sheet constructs. Furthermore, increased ECM deposition was observed in co-culture groups. PDLSCs and HUVECs seeded on Matrigel showed initiation of vessel like structure formation after 4 hours. Extensive lattice of vessel like structures was formed in 1:2 and 1:5 groups compared to other groups. However, after 8 hours all the groups showed formation of island like cell aggregations. In vivo, H&E sections exemplified the successful integration of implanted PDL cell sheet coated roots with host tissues after 2 weeks. Periodontal ligament-like arrangement was observed around root surface in cell sheet groups compared to control groups. Conclusions: 3D cell sheet model demonstrated a promising regenerative potential in vivo and this approach would be useful in future periodontal bioengineering strategies. This abstract is based on research that was funded entirely or partially by an outside source: Seed Funding for Basic Research 201111159191 The University of Hong Kong | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | - |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | - |
dc.subject | Cell biology | - |
dc.subject | Periodontal disease | - |
dc.subject | Regeneration | - |
dc.subject | Stem cells and Tissue engineering | - |
dc.title | Development of Novel 3D Stem Cell Sheet for Periodontal Regeneration | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Samaranayake, LP: lakshman@hku.hk | en_US |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | en_US |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | en_US |
dc.identifier.authority | Samaranayake, LP=rp00023 | en_US |
dc.identifier.authority | Jin, L=rp00028 | en_US |
dc.identifier.authority | Zhang, C=rp01408 | en_US |
dc.identifier.hkuros | 231069 | en_US |
dc.identifier.volume | 93 | en_US |
dc.identifier.issue | Special issue B: abstract no. 1067 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |