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Conference Paper: Peroxynitrite could regulate proliferation and neuronal differentiation of neural stem/progenitor cells through activating WNT/β-catenin signaling pathway
Title | Peroxynitrite could regulate proliferation and neuronal differentiation of neural stem/progenitor cells through activating WNT/β-catenin signaling pathway |
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Authors | |
Issue Date | 2014 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jns |
Citation | The 21st World Congress of Neurology (WCN 2013), Vienna, Austria, 20-26 September 2013. In Journal of the Neurological Sciences, 2013, v. 333 suppl. 1, p. e228 How to Cite? |
Abstract | Background: Hypoxia/ischemia could mediate the differentiation of neural stem/progenitor cells (NSCs) into mature neurons. In hypoxic/ischemic brain, nitric oxide and superoxide are simultaneously produced and they rapidly react to form peroxynitrite. Whether peroxynitrite can regulate neurogenesis is unknown yet.
Objectives: Present study aims to understand the roles of peroxynitrite in regulating self-renewal, proliferation and differentiation of NSCs.
Materials and methods: Primary cultured NSCs were subjected to different stimulations including peroxynitrite donor SIN-1, synthesized peroxynitrite and hypoxia treatment. For visualizing the formation of peroxynitrite, we developed a highly sensitive and specific fluorescent probe and detected the formation of peroxynitrite in the NSCs. We applied different biomarkers including Ki67, BrdU, Tuj1 and DCX, etc./INS; to identify the self-renewal proliferation, and neuronal differentiation of NSCs respectively.
Results: Low concentrations of extraneous peroxynitrite (< 1 μM) promoted NSC/INS; proliferation, self-renewal and neuronal differentiation but high level of peroxynitrite (> 5 μM) induced cytotoxicity. Hypoxic treatment induced the production of peroxynitrite and promoted NSC/INS; proliferation and self-renewal, and neuronal differentiation. Treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) reduced hypoxia-induced peroxynitrite formation, NSC/INS; proliferation, self-renewal and neuronal differentiation. The neurogenesis promoting effects were partly mediated through activating Wnt/β-catenin signaling pathway.
Conclusion: Low concentration of peroxynitrite may serve as a cellular signaling for promoting NSC/INS; proliferation, self-renewal and neuronal differentiation.
Copyright © 2013 Published by Elsevier B.V. |
Description | Conference Theme: Neurology in the Age of Globalization |
Persistent Identifier | http://hdl.handle.net/10722/201702 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.042 |
DC Field | Value | Language |
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dc.contributor.author | Shen, J | en_US |
dc.contributor.author | Chen, XM | - |
dc.contributor.author | Yan, TT | - |
dc.date.accessioned | 2014-08-21T07:37:37Z | - |
dc.date.available | 2014-08-21T07:37:37Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | The 21st World Congress of Neurology (WCN 2013), Vienna, Austria, 20-26 September 2013. In Journal of the Neurological Sciences, 2013, v. 333 suppl. 1, p. e228 | en_US |
dc.identifier.issn | 0022-510X | - |
dc.identifier.uri | http://hdl.handle.net/10722/201702 | - |
dc.description | Conference Theme: Neurology in the Age of Globalization | - |
dc.description.abstract | Background: Hypoxia/ischemia could mediate the differentiation of neural stem/progenitor cells (NSCs) into mature neurons. In hypoxic/ischemic brain, nitric oxide and superoxide are simultaneously produced and they rapidly react to form peroxynitrite. Whether peroxynitrite can regulate neurogenesis is unknown yet. Objectives: Present study aims to understand the roles of peroxynitrite in regulating self-renewal, proliferation and differentiation of NSCs. Materials and methods: Primary cultured NSCs were subjected to different stimulations including peroxynitrite donor SIN-1, synthesized peroxynitrite and hypoxia treatment. For visualizing the formation of peroxynitrite, we developed a highly sensitive and specific fluorescent probe and detected the formation of peroxynitrite in the NSCs. We applied different biomarkers including Ki67, BrdU, Tuj1 and DCX, etc./INS; to identify the self-renewal proliferation, and neuronal differentiation of NSCs respectively. Results: Low concentrations of extraneous peroxynitrite (< 1 μM) promoted NSC/INS; proliferation, self-renewal and neuronal differentiation but high level of peroxynitrite (> 5 μM) induced cytotoxicity. Hypoxic treatment induced the production of peroxynitrite and promoted NSC/INS; proliferation and self-renewal, and neuronal differentiation. Treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) reduced hypoxia-induced peroxynitrite formation, NSC/INS; proliferation, self-renewal and neuronal differentiation. The neurogenesis promoting effects were partly mediated through activating Wnt/β-catenin signaling pathway. Conclusion: Low concentration of peroxynitrite may serve as a cellular signaling for promoting NSC/INS; proliferation, self-renewal and neuronal differentiation. Copyright © 2013 Published by Elsevier B.V. | - |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jns | - |
dc.relation.ispartof | Journal of the Neurological Sciences | en_US |
dc.rights | NOTICE: this is the author’s version of a work that was accepted for publication in Journal of the Neurological Sciences. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of the Neurological Sciences, 2013, v. 333 n. Suppl. 1, p. e228 DOI# 10.1016/j.jns.2013.07.896 | - |
dc.title | Peroxynitrite could regulate proliferation and neuronal differentiation of neural stem/progenitor cells through activating WNT/β-catenin signaling pathway | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Shen, J: shenjg@hku.hk | en_US |
dc.identifier.email | Chen, XM: chenxm@hku.hk | - |
dc.identifier.authority | Shen, J=rp00487 | en_US |
dc.identifier.doi | 10.1016/j.jns.2013.07.896 | - |
dc.identifier.hkuros | 233604 | en_US |
dc.identifier.volume | 333 | - |
dc.identifier.issue | suppl. 1 | - |
dc.identifier.spage | e228 | - |
dc.identifier.epage | e228 | - |
dc.publisher.place | The Netherlands | - |
dc.identifier.issnl | 0022-510X | - |