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Conference Paper: Mitochondrial Transfer of Induced Pluripotent Stem Cell–Derived Mesenchymal Stem Cells to Airway Epithelial Cells Attenuates Cigarette Smoke–Induced Damage
Title | Mitochondrial Transfer of Induced Pluripotent Stem Cell–Derived Mesenchymal Stem Cells to Airway Epithelial Cells Attenuates Cigarette Smoke–Induced Damage |
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Authors | |
Issue Date | 2014 |
Publisher | American Thoracic Society. The Conference Abstracts web site is located at http://www.atsjournals.org/series/ajrccm-conference |
Citation | The 2014 International Conference of the American Thoracic Society (ATS), San Diego, CA., 16-21 May 2014. In American Journal of Respiratory and Critical Care Medicine, 2014, v. 189, abstract no. A5295 How to Cite? |
Abstract | Transplantation of mesenchymal stem cells (MSCs) holds great promise in the repair of cigarette smoke (CS)-induced lung damage in chronic obstructive pulmonary disease (COPD). Because CS leads to mitochondrial dysfunction, we aimed to investigate the potential benefit of mitochondrial transfer from human-induced pluripotent stem cell–derived MSCs (iPSC-MSCs) to CS-exposed airway epithelial cells in vitro and in vivo. Rats were exposed to 4% CS for 1 hour daily for 56 days. At Days 29 and, human iPSC-MSCs or adult bone marrow–derived MSCs (BM-MSCs) were administered intravenously to CS-exposed rats. CS-exposed rats exhibited severe alveolar destruction with a higher mean linear intercept (Lm) than sham air–exposed rats (P < 0.001) that was attenuated in the presence of iPSC-MSCs or BM-MSCs (P < 0.01). The attenuation of Lm value and the severity of fibrosis was greater in the iPSC-MSC–treated group than in the BM-MSC–treated group (P < 0.05). This might have contributed to the novel observation of mitochondrial transfer from MSCs to rat airway epithelial cells in lung sections exposed to CS. In vitro studies further revealed that transfer of mitochondria from iPSC-MSCs to bronchial epithelial cells (BEAS-2B) was more effective than from BM-MSCs, with preservation of adenosine triphosphate contents. This distinct mitochondrial transfer occurred via the formation of tunneling nanotubes. Inhibition of tunneling nanotube formation blocked mitochondrial transfer. Our findings indicate a higher mitochondrial transfer capacity of iPSC-MSCs than BM-MSCs to rescue CS-induced mitochondrial damage. iPSC-MSCs may thus hold promise for the development of cell therapy in COPD.
Read More: http://www.atsjournals.org/doi/abs/10.1165/rcmb.2013-0529OC#.VFyVL_mUd1Y |
Description | Poster Discussion Session C108: Is There Anything They Can't Do? Diverse Functional Activities of Mesenchymal Stem and Stromal Cells |
Persistent Identifier | http://hdl.handle.net/10722/202100 |
ISSN | 2023 Impact Factor: 19.3 2023 SCImago Journal Rankings: 5.336 |
DC Field | Value | Language |
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dc.contributor.author | Li, X | en_US |
dc.contributor.author | Zhang, Y | en_US |
dc.contributor.author | Liang, Y | en_US |
dc.contributor.author | Yeung, SC | en_US |
dc.contributor.author | Ip, MSM | en_US |
dc.contributor.author | Tse, HF | en_US |
dc.contributor.author | Lian, Q | en_US |
dc.contributor.author | Mak, JCW | en_US |
dc.date.accessioned | 2014-08-21T08:03:50Z | - |
dc.date.available | 2014-08-21T08:03:50Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | The 2014 International Conference of the American Thoracic Society (ATS), San Diego, CA., 16-21 May 2014. In American Journal of Respiratory and Critical Care Medicine, 2014, v. 189, abstract no. A5295 | en_US |
dc.identifier.issn | 1073-449X | - |
dc.identifier.uri | http://hdl.handle.net/10722/202100 | - |
dc.description | Poster Discussion Session C108: Is There Anything They Can't Do? Diverse Functional Activities of Mesenchymal Stem and Stromal Cells | - |
dc.description.abstract | Transplantation of mesenchymal stem cells (MSCs) holds great promise in the repair of cigarette smoke (CS)-induced lung damage in chronic obstructive pulmonary disease (COPD). Because CS leads to mitochondrial dysfunction, we aimed to investigate the potential benefit of mitochondrial transfer from human-induced pluripotent stem cell–derived MSCs (iPSC-MSCs) to CS-exposed airway epithelial cells in vitro and in vivo. Rats were exposed to 4% CS for 1 hour daily for 56 days. At Days 29 and, human iPSC-MSCs or adult bone marrow–derived MSCs (BM-MSCs) were administered intravenously to CS-exposed rats. CS-exposed rats exhibited severe alveolar destruction with a higher mean linear intercept (Lm) than sham air–exposed rats (P < 0.001) that was attenuated in the presence of iPSC-MSCs or BM-MSCs (P < 0.01). The attenuation of Lm value and the severity of fibrosis was greater in the iPSC-MSC–treated group than in the BM-MSC–treated group (P < 0.05). This might have contributed to the novel observation of mitochondrial transfer from MSCs to rat airway epithelial cells in lung sections exposed to CS. In vitro studies further revealed that transfer of mitochondria from iPSC-MSCs to bronchial epithelial cells (BEAS-2B) was more effective than from BM-MSCs, with preservation of adenosine triphosphate contents. This distinct mitochondrial transfer occurred via the formation of tunneling nanotubes. Inhibition of tunneling nanotube formation blocked mitochondrial transfer. Our findings indicate a higher mitochondrial transfer capacity of iPSC-MSCs than BM-MSCs to rescue CS-induced mitochondrial damage. iPSC-MSCs may thus hold promise for the development of cell therapy in COPD. Read More: http://www.atsjournals.org/doi/abs/10.1165/rcmb.2013-0529OC#.VFyVL_mUd1Y | - |
dc.language | eng | en_US |
dc.publisher | American Thoracic Society. The Conference Abstracts web site is located at http://www.atsjournals.org/series/ajrccm-conference | - |
dc.relation.ispartof | American Journal of Respiratory and Critical Care Medicine | en_US |
dc.title | Mitochondrial Transfer of Induced Pluripotent Stem Cell–Derived Mesenchymal Stem Cells to Airway Epithelial Cells Attenuates Cigarette Smoke–Induced Damage | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Yeung, SC: flag@hkucc.hku.hk | en_US |
dc.identifier.email | Ip, MSM: msmip@hku.hk | en_US |
dc.identifier.email | Tse, HF: hftse@hkucc.hku.hk | en_US |
dc.identifier.email | Lian, Q: qzlian@hkucc.hku.hk | en_US |
dc.identifier.email | Mak, JCW: judithmak@hku.hk | en_US |
dc.identifier.authority | Ip, MSM=rp00347 | en_US |
dc.identifier.authority | Tse, HF=rp00428 | en_US |
dc.identifier.authority | Lian, Q=rp00267 | en_US |
dc.identifier.authority | Mak, JCW=rp00352 | en_US |
dc.identifier.hkuros | 234872 | en_US |
dc.identifier.volume | 189 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1073-449X | - |