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- PMID: 24358269
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Article: A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice
Title | A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice |
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Authors | |
Issue Date | 2013 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | PLoS ONE, 2013, v. 8 n. 12, article no. e83274 How to Cite? |
Abstract | To combat the possibility of a zoonotic H5N1 pandemic in a timely fashion, it is necessary to develop a vaccine that would confer protection against homologous and heterologous human H5N1 influenza viruses. Using a replicating modified vaccinia virus Tian Tan strain (MVTT) as a vaccine vector, we constructed MVTTHA-QH and MVTTHA-AH, which expresses the H5 gene of a goose-derived Qinghai strain A/Bar-headed Goose/Qinghai/1/2005 or human-derived Anhui Strain A/Anhui/1/2005. The immunogenicity profiles of both vaccine candidates were evaluated. Vaccination with MVTTHA-QH induced a significant level of neutralizing antibodies (Nabs) against a homologous strain and a wide range of H5N1 pseudoviruses (clades 1, 2.1, 2.2, 2.3.2, and 2.3.4). Neutralization tests (NT) and Haemagglutination inhibition (HI) antibodies inhibit the live autologous virus as well as a homologous A/Xingjiang/1/2006 and a heterologous A/Vietnam/1194/2004, representing two human isolates from clade 2.2 and clade 1, respectively. Importantly, mice vaccinated with intranasal MVTTHA-QH were completely protected from challenge with lethal dosages of A/Bar-headed Goose/Qinghai/1/2005 and the A/Viet Nam/1194/2004, respectively, but not control mice that received a mock MVTTS vaccine. However, MVTTHA-AH induced much lower levels of NT against its autologous strain. Our results suggest that it is feasible to use the H5 gene from A/Bar-headed Goose/Qinghai/1/2005 to construct an effective vaccine, when using MVTT as a vector, to prevent infections against homologous and genetically divergent human H5N1 influenza viruses. |
Persistent Identifier | http://hdl.handle.net/10722/203172 |
PubMed Central ID | |
ISI Accession Number ID | |
Errata |
DC Field | Value | Language |
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dc.contributor.author | Xiao, H | en_US |
dc.contributor.author | Liu, L | en_US |
dc.contributor.author | Zhu, Q | en_US |
dc.contributor.author | Tan, Z | en_US |
dc.contributor.author | Yu, W | en_US |
dc.contributor.author | Tang, X | en_US |
dc.contributor.author | Zhan, D | en_US |
dc.contributor.author | Du, Y | en_US |
dc.contributor.author | Wang, H | en_US |
dc.contributor.author | Liu, D | en_US |
dc.contributor.author | Li, Z | en_US |
dc.contributor.author | Yuen, KY | en_US |
dc.contributor.author | Ho, DD | en_US |
dc.contributor.author | Gao, GF | en_US |
dc.contributor.author | Chen, Z | en_US |
dc.date.accessioned | 2014-09-19T12:56:44Z | - |
dc.date.available | 2014-09-19T12:56:44Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | PLoS ONE, 2013, v. 8 n. 12, article no. e83274 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/203172 | - |
dc.description.abstract | To combat the possibility of a zoonotic H5N1 pandemic in a timely fashion, it is necessary to develop a vaccine that would confer protection against homologous and heterologous human H5N1 influenza viruses. Using a replicating modified vaccinia virus Tian Tan strain (MVTT) as a vaccine vector, we constructed MVTTHA-QH and MVTTHA-AH, which expresses the H5 gene of a goose-derived Qinghai strain A/Bar-headed Goose/Qinghai/1/2005 or human-derived Anhui Strain A/Anhui/1/2005. The immunogenicity profiles of both vaccine candidates were evaluated. Vaccination with MVTTHA-QH induced a significant level of neutralizing antibodies (Nabs) against a homologous strain and a wide range of H5N1 pseudoviruses (clades 1, 2.1, 2.2, 2.3.2, and 2.3.4). Neutralization tests (NT) and Haemagglutination inhibition (HI) antibodies inhibit the live autologous virus as well as a homologous A/Xingjiang/1/2006 and a heterologous A/Vietnam/1194/2004, representing two human isolates from clade 2.2 and clade 1, respectively. Importantly, mice vaccinated with intranasal MVTTHA-QH were completely protected from challenge with lethal dosages of A/Bar-headed Goose/Qinghai/1/2005 and the A/Viet Nam/1194/2004, respectively, but not control mice that received a mock MVTTS vaccine. However, MVTTHA-AH induced much lower levels of NT against its autologous strain. Our results suggest that it is feasible to use the H5 gene from A/Bar-headed Goose/Qinghai/1/2005 to construct an effective vaccine, when using MVTT as a vector, to prevent infections against homologous and genetically divergent human H5N1 influenza viruses. | - |
dc.language | eng | en_US |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | - |
dc.relation.ispartof | PLoS ONE | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice | en_US |
dc.type | Article | en_US |
dc.identifier.email | Liu, L: liuli71@hkucc.hku.hk | en_US |
dc.identifier.email | Du, DY: biodu@hku.hk | en_US |
dc.identifier.email | Wang, H: hbwang@hkucc.hku.hk | en_US |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | en_US |
dc.identifier.email | Chen, Z: zchenai@hku.hk | en_US |
dc.identifier.authority | Liu, L=rp00268 | en_US |
dc.identifier.authority | Yuen, KY=rp00366 | en_US |
dc.identifier.authority | Chen, Z=rp00243 | en_US |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0083274 | en_US |
dc.identifier.pmid | 24358269 | - |
dc.identifier.pmcid | PMC3866202 | - |
dc.identifier.scopus | eid_2-s2.0-84892924704 | - |
dc.identifier.hkuros | 237094 | en_US |
dc.identifier.volume | 8 | en_US |
dc.identifier.issue | 12 | - |
dc.identifier.spage | article no. e83274 | - |
dc.identifier.epage | article no. e83274 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.isi | WOS:000328737700055 | - |
dc.relation.erratum | doi:10.1371/journal.pone.0115925 | - |
dc.relation.erratum | eid:eid_2-s2.0-84917691152 | - |
dc.identifier.issnl | 1932-6203 | - |