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Article: Down-regulated CK8 expression in human intervertebral disc degeneration

TitleDown-regulated CK8 expression in human intervertebral disc degeneration
Authors
KeywordsCell clusters
Cytokeratin 8
Immunofluorescence
Intervertebral disc degeneration
Nucleus pulposus
qRT-PCR
Issue Date2013
PublisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/
Citation
International Journal of Medical Sciences, 2013, v. 10 n. 8, p. 948-956 How to Cite?
AbstractAs an intermediate filament protein, cytokeratin 8 (CK8) exerts multiple cellular functions. Moreover, it has been identified as a marker of notochord cells, which play essential roles in human nucleus pulposus (NP). However, the distribution of CK8 positive cells in human NP and their relationship with intervertebral disc degeneration (IDD) have not been clarified until now. Here, we found the percentage of CK8 positive cells in IDD (25.7+/-4.14%) was significantly lower than that in normal and scoliosis NP (51.9+/-9.73% and 47.8+/-5.51%, respectively, p<0.05). Western blotting and qRT-PCR results confirmed the down-regulation of CK8 expression in IDD on both of protein and mRNA levels. Furthermore, approximately 37.4% of cell clusters were CK8 positive in IDD. Taken together, this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence. Consequently, CK8 positive cells might be considered as a potential option in the development of cellular treatment strategies for NP repair.
Persistent Identifierhttp://hdl.handle.net/10722/203239
ISSN
2019 Impact Factor: 2.523
2015 SCImago Journal Rankings: 0.813
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Z-
dc.contributor.authorWang, HQ-
dc.contributor.authorLiu, ZH-
dc.contributor.authorChang, L-
dc.contributor.authorChen, YF-
dc.contributor.authorZhang, YZ-
dc.contributor.authorZhang, WL-
dc.contributor.authorGao, Y-
dc.contributor.authorWan, ZY-
dc.contributor.authorChe, L-
dc.contributor.authorLiu, X-
dc.contributor.authorSamartzis, D-
dc.contributor.authorLuo, ZJ-
dc.date.accessioned2014-09-19T13:11:20Z-
dc.date.available2014-09-19T13:11:20Z-
dc.date.issued2013-
dc.identifier.citationInternational Journal of Medical Sciences, 2013, v. 10 n. 8, p. 948-956-
dc.identifier.issn1449-1907-
dc.identifier.urihttp://hdl.handle.net/10722/203239-
dc.description.abstractAs an intermediate filament protein, cytokeratin 8 (CK8) exerts multiple cellular functions. Moreover, it has been identified as a marker of notochord cells, which play essential roles in human nucleus pulposus (NP). However, the distribution of CK8 positive cells in human NP and their relationship with intervertebral disc degeneration (IDD) have not been clarified until now. Here, we found the percentage of CK8 positive cells in IDD (25.7+/-4.14%) was significantly lower than that in normal and scoliosis NP (51.9+/-9.73% and 47.8+/-5.51%, respectively, p<0.05). Western blotting and qRT-PCR results confirmed the down-regulation of CK8 expression in IDD on both of protein and mRNA levels. Furthermore, approximately 37.4% of cell clusters were CK8 positive in IDD. Taken together, this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence. Consequently, CK8 positive cells might be considered as a potential option in the development of cellular treatment strategies for NP repair.-
dc.languageeng-
dc.publisherIvyspring International Publisher. The Journal's web site is located at http://www.medsci.org/-
dc.relation.ispartofInternational Journal of Medical Sciences-
dc.rightsInternational Journal of Medical Sciences. Copyright © Ivyspring International Publisher.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCell clusters-
dc.subjectCytokeratin 8-
dc.subjectImmunofluorescence-
dc.subjectIntervertebral disc degeneration-
dc.subjectNucleus pulposus-
dc.subjectqRT-PCR-
dc.subject.meshDown-Regulation-
dc.subject.meshIntervertebral Disc Degeneration - genetics-
dc.subject.meshKeratin-8 - genetics-
dc.subject.meshReal-Time Polymerase Chain Reaction-
dc.titleDown-regulated CK8 expression in human intervertebral disc degeneration-
dc.typeArticle-
dc.identifier.emailSamartzis, D: dspine@hku.hk-
dc.identifier.authoritySamartzis, D=rp01430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7150/ijms.5642-
dc.identifier.pmid23801880-
dc.identifier.pmcidPMC3691792-
dc.identifier.scopuseid_2-s2.0-84879052848-
dc.identifier.hkuros237994-
dc.identifier.volume10-
dc.identifier.issue8-
dc.identifier.spage948-
dc.identifier.epage956-
dc.identifier.isiWOS:000321514900002-
dc.publisher.placeAustralia-
dc.identifier.issnl1449-1907-

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