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Book Chapter: Portable Two-Way Riboswitches: Design and Engineering

TitlePortable Two-Way Riboswitches: Design and Engineering
Authors
KeywordsLacI
LacP
Portable two-way regulation
Riboswitch
Issue Date2014
PublisherHumana Press
Citation
Portable Two-Way Riboswitches: Design and Engineering. In Ogawa, A (Ed.), Artificial Riboswitches: Methods and Protocols, p. 93-106. New York: Humana Press, 2014 How to Cite?
AbstractRiboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.
Persistent Identifierhttp://hdl.handle.net/10722/205461
ISBN
ISSN
2015 SCImago Journal Rankings: 0.549
Series/Report no.Methods in Molecular Biology; vol. 1111

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_US
dc.contributor.authorHuang, Jen_US
dc.date.accessioned2014-09-20T02:35:38Z-
dc.date.available2014-09-20T02:35:38Z-
dc.date.issued2014en_US
dc.identifier.citationPortable Two-Way Riboswitches: Design and Engineering. In Ogawa, A (Ed.), Artificial Riboswitches: Methods and Protocols, p. 93-106. New York: Humana Press, 2014en_US
dc.identifier.isbn9781627037549en_US
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/205461-
dc.description.abstractRiboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.-
dc.languageengen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofArtificial Riboswitches: Methods and Protocolsen_US
dc.relation.ispartofseriesMethods in Molecular Biology; vol. 1111-
dc.subjectLacI-
dc.subjectLacP-
dc.subjectPortable two-way regulation-
dc.subjectRiboswitch-
dc.titlePortable Two-Way Riboswitches: Design and Engineeringen_US
dc.typeBook_Chapteren_US
dc.identifier.emailJin, Y: jinye@hkusua.hku.hken_US
dc.identifier.emailHuang, J: jdhuang@hku.hken_US
dc.identifier.authorityHuang, J=rp00451en_US
dc.identifier.doi10.1007/978-1-62703-755-6_7-
dc.identifier.scopuseid_2-s2.0-84934439653-
dc.identifier.hkuros237848en_US
dc.identifier.spage93en_US
dc.identifier.epage106en_US
dc.identifier.eissn1940-6029-
dc.publisher.placeNew York-
dc.identifier.issnl1064-3745-

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