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- Publisher Website: 10.1021/ja8087407
- Scopus: eid_2-s2.0-67651211363
- PMID: 19621955
- WOS: WOS:000268395000043
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Article: Electron ionization dissociation of singly and multiply charged peptides
Title | Electron ionization dissociation of singly and multiply charged peptides |
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Authors | |
Issue Date | 2009 |
Citation | Journal of the American Chemical Society, 2009, v. 131, n. 29, p. 9977-9985 How to Cite? |
Abstract | A new tandem mass spectrometry technique, electron ionization dissociation (EID), employs irradiation of trapped cations [M + nH]n+ (n ≥ 1) by fast electrons with energy at least 10 eV higher than the ionization threshold of the cations. Such irradiation causes simultaneous ionization and electronic excitation of the irradiated species, which is equivalent of double-ionization to [M + nH](n+2)+ followed by electron capture to form electronically excited [M + nH](n+1)+ * • ions. Subsequent fragmentation of the latter species gives both side-chain losses and backbone fragmentation. Theoretical efficiency of such fragmentation calculated as a ratio of the fragment ion current and the precursor ion depletion can reach (n+1)/n, that is, can exceed 100%. EID often leads to backbone N-Cα bond cleavage, giving c-/z-type fragments. C-C bond cleavage, giving preferentially a-/x-type fragments, is also observed. EID could be used in bottom-up proteomics for both electrospray and matrix-assisted laser desorption ionization (MALDI) -produced ions. The energy incorporated into the precursor ion in a single interaction with electrons can exceed 10 eV, which makes EID suitable for top-down analysis of folded gas-phase protein conformations. © 2009 American Chemical Society. |
Persistent Identifier | http://hdl.handle.net/10722/206233 |
ISSN | 2023 Impact Factor: 14.4 2023 SCImago Journal Rankings: 5.489 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Fung, Yi Man Eva | - |
dc.contributor.author | Adams, Christopher M. | - |
dc.contributor.author | Zubarev, Roman A. | - |
dc.date.accessioned | 2014-10-22T01:25:29Z | - |
dc.date.available | 2014-10-22T01:25:29Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Journal of the American Chemical Society, 2009, v. 131, n. 29, p. 9977-9985 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/10722/206233 | - |
dc.description.abstract | A new tandem mass spectrometry technique, electron ionization dissociation (EID), employs irradiation of trapped cations [M + nH]n+ (n ≥ 1) by fast electrons with energy at least 10 eV higher than the ionization threshold of the cations. Such irradiation causes simultaneous ionization and electronic excitation of the irradiated species, which is equivalent of double-ionization to [M + nH](n+2)+ followed by electron capture to form electronically excited [M + nH](n+1)+ * • ions. Subsequent fragmentation of the latter species gives both side-chain losses and backbone fragmentation. Theoretical efficiency of such fragmentation calculated as a ratio of the fragment ion current and the precursor ion depletion can reach (n+1)/n, that is, can exceed 100%. EID often leads to backbone N-Cα bond cleavage, giving c-/z-type fragments. C-C bond cleavage, giving preferentially a-/x-type fragments, is also observed. EID could be used in bottom-up proteomics for both electrospray and matrix-assisted laser desorption ionization (MALDI) -produced ions. The energy incorporated into the precursor ion in a single interaction with electrons can exceed 10 eV, which makes EID suitable for top-down analysis of folded gas-phase protein conformations. © 2009 American Chemical Society. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of the American Chemical Society | - |
dc.title | Electron ionization dissociation of singly and multiply charged peptides | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/ja8087407 | - |
dc.identifier.pmid | 19621955 | - |
dc.identifier.scopus | eid_2-s2.0-67651211363 | - |
dc.identifier.volume | 131 | - |
dc.identifier.issue | 29 | - |
dc.identifier.spage | 9977 | - |
dc.identifier.epage | 9985 | - |
dc.identifier.isi | WOS:000268395000043 | - |
dc.identifier.issnl | 0002-7863 | - |