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Article: Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate
Title | Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate |
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Authors | |
Issue Date | 1984 |
Publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://molpharm.aspetjournals.org/ |
Citation | Moelcular Pharmacology, 1984, v. 26 n. 2, p. 255-260 How to Cite? |
Abstract | Incubation of primary cultures of chromaffin cells from bovine adrenal medulla with 8-bromo-adenosine 3',5'-monophosphate (8-Br-cyclic AMP) resulted in an increase in proenkephalin mRNA content. The mRNA that increased was detected by hybridization analysis using a cDNA probe and migrated with an apparent size of approximately 1400 bases. The increase in proenkephalin mRNA following 8-Br-cyclic AMP treatment was apparent in 12 hr and continued over 2 days. Corresponding changes were detected in enkephalin-like immunoreactivity but with a 24-hr lag: the cellular content increased significantly after 2 days of treatment and continued to rise over the next 2 days, whereas changes in the amount released to the medium followed the same time course. Dose-response curves for the increase in the content of proenkephalin mRNA and of enkephalin-containing peptides were essentially identical. Chromatographic characterization of the enkephalin-like peptides demonstrated that 8-Br-cyclic AMP increased both the high molecular weight fraction and the low molecular weight fraction, which was shown by high-pressure liquid chromatography to contain Met5-enkephalin, Leu5-enkephalin, and Met5-enkephalin-Arg6-Phe7. Previous results in chromaffin cells have demonstrated that the synthesis of tyrosine hydroxylase is also regulated by cyclic AMP, with a similar time course. These results therefore suggest the possibility of coordinate regulation by cyclic AMP of the expression of the cotransmitters, catecholamines and enkephalin peptides, in the adrenal medulla. |
Persistent Identifier | http://hdl.handle.net/10722/207990 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 1.038 |
DC Field | Value | Language |
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dc.contributor.author | Quach, TT | - |
dc.contributor.author | Tang, F | - |
dc.contributor.author | Kageyama, H | - |
dc.contributor.author | Mochetti, I | - |
dc.contributor.author | Guidotti, A | - |
dc.contributor.author | Meek, JL | - |
dc.contributor.author | Costa, E | - |
dc.date.accessioned | 2015-02-05T10:12:54Z | - |
dc.date.available | 2015-02-05T10:12:54Z | - |
dc.date.issued | 1984 | - |
dc.identifier.citation | Moelcular Pharmacology, 1984, v. 26 n. 2, p. 255-260 | - |
dc.identifier.issn | 0026-895X | - |
dc.identifier.uri | http://hdl.handle.net/10722/207990 | - |
dc.description.abstract | Incubation of primary cultures of chromaffin cells from bovine adrenal medulla with 8-bromo-adenosine 3',5'-monophosphate (8-Br-cyclic AMP) resulted in an increase in proenkephalin mRNA content. The mRNA that increased was detected by hybridization analysis using a cDNA probe and migrated with an apparent size of approximately 1400 bases. The increase in proenkephalin mRNA following 8-Br-cyclic AMP treatment was apparent in 12 hr and continued over 2 days. Corresponding changes were detected in enkephalin-like immunoreactivity but with a 24-hr lag: the cellular content increased significantly after 2 days of treatment and continued to rise over the next 2 days, whereas changes in the amount released to the medium followed the same time course. Dose-response curves for the increase in the content of proenkephalin mRNA and of enkephalin-containing peptides were essentially identical. Chromatographic characterization of the enkephalin-like peptides demonstrated that 8-Br-cyclic AMP increased both the high molecular weight fraction and the low molecular weight fraction, which was shown by high-pressure liquid chromatography to contain Met5-enkephalin, Leu5-enkephalin, and Met5-enkephalin-Arg6-Phe7. Previous results in chromaffin cells have demonstrated that the synthesis of tyrosine hydroxylase is also regulated by cyclic AMP, with a similar time course. These results therefore suggest the possibility of coordinate regulation by cyclic AMP of the expression of the cotransmitters, catecholamines and enkephalin peptides, in the adrenal medulla. | en_US |
dc.language | eng | - |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://molpharm.aspetjournals.org/ | - |
dc.relation.ispartof | Moelcular Pharmacology | - |
dc.title | Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate | en_US |
dc.type | Article | en_US |
dc.identifier.pmid | 6548292 | - |
dc.identifier.volume | 26 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 255 | - |
dc.identifier.epage | 260 | - |
dc.identifier.issnl | 0026-895X | - |