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Article: Enzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil
Title | Enzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil |
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Authors | |
Keywords | Enrichment enzymatic fatty acid selectivity lipase polyunsaturated fatty acids |
Issue Date | 1995 |
Publisher | Springer New York LLC. |
Citation | Journal of Oil & Fat Industries, 1995, v. 72 n. 2, p. 245-249 How to Cite? |
Abstract | Enrichment ofcis-5 polyunsaturated fatty acids [20:3(5c,11c,14c), 4.3% and 20:4(5c,11c,14c,17c), 11.3%] fromBiota orientalis seed oil was carried out by lipase-catalyzed selective esterification and hydrolysis reactions. Lipases fromRhizomucor miehei (Lipozyme),Candida cylindracea and porcine pancreas were used. Lipozyme-catalyzed esterification ofBiota fatty acids withn-butanol inn-hexane allowed 20:3 and 20:4 (as fatty acids) to be enriched to a maximum level of 52.9%, and in the presence ofC. cylindracea lipase 61.5% enrichment was achieved. Esterification with pancreatic lipase was poor with low levels of enrichment of 20:3
and 20:4 (22%). A multigram scale esterification of the free fatty acids fromBiota seed oil by repeated treatment of the isolated fatty acid fraction withn-butanol inn-hexane in the presence ofC. cylindracea lipase furnished an enrichment yield of 72.5% of a mixture of 20:3 and 20:4 fatty acids. Urea fractionation of the free fatty
acids ofBiota oil gave an initial enriched fraction of 20:3 (9.5%) and 20:4 (25.2%) which, upon treatment withC. cylindracea lipase inn-butanol andn-hexane, gave an enriched fraction of 85.3% of 20:3 and 20:4 fatty acids. Partial hydrolysis of the triglycerides ofBiota oil byC. cylindracea lipase in potassium phosphate buffer at 25°C resulted in a 2.8-fold enrichment ofcis-5 polyunsaturated fatty acids (40.8% of 20:3 and 20:4) as contained in the unhydrolyzed acylglycerol fractions. |
Persistent Identifier | http://hdl.handle.net/10722/209159 |
ISSN |
DC Field | Value | Language |
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dc.contributor.author | Lie Ken Jie, MSF | - |
dc.contributor.author | Syed Rahmatullah, MSK | - |
dc.date.accessioned | 2015-04-01T07:00:11Z | - |
dc.date.available | 2015-04-01T07:00:11Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Journal of Oil & Fat Industries, 1995, v. 72 n. 2, p. 245-249 | - |
dc.identifier.issn | 0095-9774 | - |
dc.identifier.uri | http://hdl.handle.net/10722/209159 | - |
dc.description.abstract | Enrichment ofcis-5 polyunsaturated fatty acids [20:3(5c,11c,14c), 4.3% and 20:4(5c,11c,14c,17c), 11.3%] fromBiota orientalis seed oil was carried out by lipase-catalyzed selective esterification and hydrolysis reactions. Lipases fromRhizomucor miehei (Lipozyme),Candida cylindracea and porcine pancreas were used. Lipozyme-catalyzed esterification ofBiota fatty acids withn-butanol inn-hexane allowed 20:3 and 20:4 (as fatty acids) to be enriched to a maximum level of 52.9%, and in the presence ofC. cylindracea lipase 61.5% enrichment was achieved. Esterification with pancreatic lipase was poor with low levels of enrichment of 20:3 and 20:4 (22%). A multigram scale esterification of the free fatty acids fromBiota seed oil by repeated treatment of the isolated fatty acid fraction withn-butanol inn-hexane in the presence ofC. cylindracea lipase furnished an enrichment yield of 72.5% of a mixture of 20:3 and 20:4 fatty acids. Urea fractionation of the free fatty acids ofBiota oil gave an initial enriched fraction of 20:3 (9.5%) and 20:4 (25.2%) which, upon treatment withC. cylindracea lipase inn-butanol andn-hexane, gave an enriched fraction of 85.3% of 20:3 and 20:4 fatty acids. Partial hydrolysis of the triglycerides ofBiota oil byC. cylindracea lipase in potassium phosphate buffer at 25°C resulted in a 2.8-fold enrichment ofcis-5 polyunsaturated fatty acids (40.8% of 20:3 and 20:4) as contained in the unhydrolyzed acylglycerol fractions. | - |
dc.language | eng | - |
dc.publisher | Springer New York LLC. | - |
dc.relation.ispartof | Journal of Oil & Fat Industries | - |
dc.rights | The original publication is available at www.springerlink.com | - |
dc.subject | Enrichment | - |
dc.subject | enzymatic | - |
dc.subject | fatty acid selectivity | - |
dc.subject | lipase | - |
dc.subject | polyunsaturated fatty acids | - |
dc.title | Enzymatic renrichment of C20 cis-5 polyunsaturated fatty acids from biota orientalis seed oil | - |
dc.type | Article | - |
dc.identifier.email | Lie Ken Jie, MSF: hrsclkj@hku.hk | - |
dc.identifier.doi | 10.1007/BF02638907 | - |
dc.identifier.scopus | eid_2-s2.0-0342527007 | - |
dc.identifier.hkuros | 514 | - |
dc.identifier.volume | 72 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 245 | - |
dc.identifier.epage | 249 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0095-9774 | - |