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Article: Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo

TitleFluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo
Authors
Issue Date2015
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html
Citation
Journal of the American Chemical Society, 2015, v. 137 n. 21, p. 6837-6843 How to Cite?
AbstractSuperoxide anion radical (O2•–) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2•– detecting strategy involving O2•– cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2•– fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2•– over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2•– in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2•– in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2•– in health and disease.
Persistent Identifierhttp://hdl.handle.net/10722/211716
ISSN
2020 Impact Factor: 15.419
2015 SCImago Journal Rankings: 7.123
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHu, JJ-
dc.contributor.authorWong, NK-
dc.contributor.authorYe, S-
dc.contributor.authorChen, X-
dc.contributor.authorLu, MY-
dc.contributor.authorZhao, AQ-
dc.contributor.authorGuo, Y-
dc.contributor.authorMa, ACH-
dc.contributor.authorLeung, AYH-
dc.contributor.authorShen, J-
dc.contributor.authorYang, D-
dc.date.accessioned2015-07-21T02:08:50Z-
dc.date.available2015-07-21T02:08:50Z-
dc.date.issued2015-
dc.identifier.citationJournal of the American Chemical Society, 2015, v. 137 n. 21, p. 6837-6843-
dc.identifier.issn0002-7863-
dc.identifier.urihttp://hdl.handle.net/10722/211716-
dc.description.abstractSuperoxide anion radical (O2•–) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2•– detecting strategy involving O2•– cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2•– fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2•– over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2•– in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2•– in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2•– in health and disease.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html-
dc.relation.ispartofJournal of the American Chemical Society-
dc.titleFluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo-
dc.typeArticle-
dc.identifier.emailYe, S: yesen@hku.hk-
dc.identifier.emailChen, X: chenxm@hku.hk-
dc.identifier.emailMa, ACH: alvinma@hku.hk-
dc.identifier.emailLeung, AYH: ayhleung@hku.hk-
dc.identifier.emailShen, J: shenjg@hku.hk-
dc.identifier.emailYang, D: yangdan@hku.hk-
dc.identifier.authorityYe, S=rp02805-
dc.identifier.authorityMa, ACH=rp01810-
dc.identifier.authorityLeung, AYH=rp00265-
dc.identifier.authorityShen, J=rp00487-
dc.identifier.authorityYang, D=rp00825-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/jacs.5b01881-
dc.identifier.pmid25988218-
dc.identifier.scopuseid_2-s2.0-84930634442-
dc.identifier.hkuros245626-
dc.identifier.volume137-
dc.identifier.issue21-
dc.identifier.spage6837-
dc.identifier.epage6843-
dc.identifier.eissn1520-5126-
dc.identifier.isiWOS:000355890600018-
dc.publisher.placeUnited States-
dc.identifier.issnl0002-7863-

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