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Article: Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells
Title | Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells |
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Authors | |
Issue Date | 2015 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | PLOS ONE, 2015, v. 10 n. 6, article no. e0128718 How to Cite? |
Abstract | Retrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar number of ganglion cells (2289 +/- 174 at 2 days) as the retrograde labeling technique from the superior colliculus (2250 +/- 94) or optic nerve stump (2279 +/- 114) after transection. This finding provides an alternative way to label retinal ganglion cells without damaging the optic tract. This will facilitate anatomical studies in identifying the morphology and connectivity of retinal ganglion cells, allowing secondary or triple labelling manipulations for long-term investigations. |
Persistent Identifier | http://hdl.handle.net/10722/212536 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.839 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liang, YX | - |
dc.contributor.author | Yang, J | - |
dc.contributor.author | Yuan, TF | - |
dc.contributor.author | So, KF | - |
dc.date.accessioned | 2015-07-21T02:39:49Z | - |
dc.date.available | 2015-07-21T02:39:49Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | PLOS ONE, 2015, v. 10 n. 6, article no. e0128718 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10722/212536 | - |
dc.description.abstract | Retrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar number of ganglion cells (2289 +/- 174 at 2 days) as the retrograde labeling technique from the superior colliculus (2250 +/- 94) or optic nerve stump (2279 +/- 114) after transection. This finding provides an alternative way to label retinal ganglion cells without damaging the optic tract. This will facilitate anatomical studies in identifying the morphology and connectivity of retinal ganglion cells, allowing secondary or triple labelling manipulations for long-term investigations. | - |
dc.language | eng | - |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | - |
dc.relation.ispartof | PLoS ONE | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells | - |
dc.type | Article | - |
dc.identifier.email | Liang, YX: yxliang@hku.hk | - |
dc.identifier.email | Yang, J: jianyang@hkucc.hku.hk | - |
dc.identifier.email | Yuan, TF: tfyuan@hku.hk | - |
dc.identifier.email | So, KF: hrmaskf@hku.hk | - |
dc.identifier.authority | Liang, YX=rp00510 | - |
dc.identifier.authority | So, KF=rp00329 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0128718 | - |
dc.identifier.pmid | 26065419 | - |
dc.identifier.pmcid | PMC4465746 | - |
dc.identifier.scopus | eid_2-s2.0-84936751022 | - |
dc.identifier.hkuros | 244317 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | article no. e0128718 | - |
dc.identifier.epage | article no. e0128718 | - |
dc.identifier.isi | WOS:000356100900028 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1932-6203 | - |