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Conference Paper: Effects of epigallocatechin gallate on smoke-induced oxidative stress and apoptosis in rat lung in vivo
Title | Effects of epigallocatechin gallate on smoke-induced oxidative stress and apoptosis in rat lung in vivo |
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Authors | |
Keywords | Medical Sciences |
Issue Date | 2015 |
Publisher | Wiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/RES |
Citation | The Airway Vista 2015, Seoul, Korea, 21–22 March 2015. In Respirology, 2015, v. 20 suppl. S1, p. 11 How to Cite? |
Abstract | BACKGROUND: Cigarette smoking is the major risk factor for the development of chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is a rich source of oxidants, and is thought to disrupt the oxidant-antioxidant balance in the lung, thus inducing apoptosis. Epigallocatechin gallate (EGCG), a natural compound found mainly in green tea, is considered as an antioxidant. The aim of this study is to explore the role of EGCG on CS-induced oxidative stress and apoptosis in a rat model of passive smoking. METHODS: Thirty-two male Sprague Dawley rats were randomly divided into four groups: Control, CS alone, EGCG alone, and combination of CS and EGCG. The control group was exposed to sham air, while the CS group was exposed to 4% CS for 1 hour daily. The EGCG and EGCG + CS groups were given EGCG (50 mg/kg; oral gavage) every other day. Rats were sacrificed 56 days later and protein was extracted from lung tissue. Protein expressions of the antioxidant enzyme quinone oxidoreductase 1 (NQO1), and apoptosisrelated protein caspase-3 (total and cleaved) and Bcl-2 were detected by Western analysis. RESULTS: CS exposure alone caused an increase in protein expression of NQO1, Bcl-2 and cleaved-caspase-3 in comparison to control group. EGCG alone increased NQO1 but has no effect on Bcl-2 or caspase-3 (total and cleaved). The combination of EGCG and CS normalized the levels of NQO1 and Bcl-2 protein expression. CONCLUSION: Our data reinforce the defensive role of EGCG against oxidative stress and apoptosis. The protective role of EGCG in reversing apoptosis in the CS-exposed rat lungs is thought to be mediated through induction of antioxidant mechanisms. |
Description | Poster abstracts This free journal suppl. entitled: Special Issue: Abstracts of the Airway Vista 2015, 21–22 March 2015, Seoul, Korea |
Persistent Identifier | http://hdl.handle.net/10722/213594 |
ISSN | 2023 Impact Factor: 6.6 2023 SCImago Journal Rankings: 1.559 |
DC Field | Value | Language |
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dc.contributor.author | Cui, Y | - |
dc.contributor.author | Liang, Y | - |
dc.contributor.author | Ip, MSM | - |
dc.contributor.author | Mak, JCW | - |
dc.date.accessioned | 2015-08-06T07:08:13Z | - |
dc.date.available | 2015-08-06T07:08:13Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The Airway Vista 2015, Seoul, Korea, 21–22 March 2015. In Respirology, 2015, v. 20 suppl. S1, p. 11 | - |
dc.identifier.issn | 1323-7799 | - |
dc.identifier.uri | http://hdl.handle.net/10722/213594 | - |
dc.description | Poster abstracts | - |
dc.description | This free journal suppl. entitled: Special Issue: Abstracts of the Airway Vista 2015, 21–22 March 2015, Seoul, Korea | - |
dc.description.abstract | BACKGROUND: Cigarette smoking is the major risk factor for the development of chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is a rich source of oxidants, and is thought to disrupt the oxidant-antioxidant balance in the lung, thus inducing apoptosis. Epigallocatechin gallate (EGCG), a natural compound found mainly in green tea, is considered as an antioxidant. The aim of this study is to explore the role of EGCG on CS-induced oxidative stress and apoptosis in a rat model of passive smoking. METHODS: Thirty-two male Sprague Dawley rats were randomly divided into four groups: Control, CS alone, EGCG alone, and combination of CS and EGCG. The control group was exposed to sham air, while the CS group was exposed to 4% CS for 1 hour daily. The EGCG and EGCG + CS groups were given EGCG (50 mg/kg; oral gavage) every other day. Rats were sacrificed 56 days later and protein was extracted from lung tissue. Protein expressions of the antioxidant enzyme quinone oxidoreductase 1 (NQO1), and apoptosisrelated protein caspase-3 (total and cleaved) and Bcl-2 were detected by Western analysis. RESULTS: CS exposure alone caused an increase in protein expression of NQO1, Bcl-2 and cleaved-caspase-3 in comparison to control group. EGCG alone increased NQO1 but has no effect on Bcl-2 or caspase-3 (total and cleaved). The combination of EGCG and CS normalized the levels of NQO1 and Bcl-2 protein expression. CONCLUSION: Our data reinforce the defensive role of EGCG against oxidative stress and apoptosis. The protective role of EGCG in reversing apoptosis in the CS-exposed rat lungs is thought to be mediated through induction of antioxidant mechanisms. | - |
dc.language | eng | - |
dc.publisher | Wiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/RES | - |
dc.relation.ispartof | Respirology | - |
dc.rights | Author holds the copyright | - |
dc.subject | Medical Sciences | - |
dc.title | Effects of epigallocatechin gallate on smoke-induced oxidative stress and apoptosis in rat lung in vivo | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Ip, MSM: msmip@hku.hk | - |
dc.identifier.email | Mak, JCW: judithmak@hku.hk | - |
dc.identifier.authority | Ip, MSM=rp00347 | - |
dc.identifier.authority | Mak, JCW=rp00352 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1111/resp.12479 | - |
dc.identifier.hkuros | 246125 | - |
dc.identifier.volume | 20 | - |
dc.identifier.issue | suppl. S1 | - |
dc.identifier.spage | 11 | - |
dc.identifier.epage | 11 | - |
dc.publisher.place | Australia | - |
dc.identifier.issnl | 1323-7799 | - |