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Conference Paper: Estradiol activated microrna 23a directly or synergistically with p53 implicated in sex difference in hepatocellular carcinoma development
Title | Estradiol activated microrna 23a directly or synergistically with p53 implicated in sex difference in hepatocellular carcinoma development |
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Authors | |
Keywords | Medical sciences Endocrinology |
Issue Date | 2015 |
Publisher | Springer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0 |
Citation | The 24th Annual Conference of the Asian Pacific Association for the Study of the Liver (APASL 2015), Istanbul, Turkey, 12-15 March 2015. In Hepatology International, 2015, v. 9 suppl. 1, p. S137, abstract no. 2102-S138 How to Cite? |
Abstract | BACKGROUND: Estrogen (E2) exerts a protective role against hepatocellular carcinoma (HCC) development. We sought to determine the effects of E2 on apoptotic miRNAs expression and explore the possible mechanism underlying apoptosis in HCC. METHODS: Microarray was performed to analyze alteration of apoptotic miRNAs in E2-treated liver cell-line. Expression profiles of selected miRNAs were verified using qRT-PCR. qRT-PCR and Western-blot were used to analyze the alteration of mRNA and protein levels of genes such as p53, ERa, XIAP and caspase-3/7. Activity of caspase3/7 was measured using a luminescent assay. RESULTS: After E2 treatment, more than twofold alteration was observed in 25 upregulated and 10 downregulated miRNAs. Expression of miR-23a in p53-mutated male cell-lines was significantly lower than cell-lines with functional p53 (all P\0.001), but not in female cells. p53 activation increased miR-23a expression in p53 +/+ HepG2 cells (P\0.0001 at 12 h. P\0.01 at 24 h), but not in p53-/-Hep3B cells. E2 via ERa could significantly activated miR- 23a (P\0.001) and p53 (P\0.01) expression, and thus replenished p53-deficiency SNU387 cells in activation of miR-23a. Moreover, miR-23a mimic and E2 significantly activated miR-23a (P\0.001) and suppressed the expression of its target XIAP (P\0.0001 and P\0.01). Decreasing of XIAP contributes to activation of caspase-3 activity and cell apoptosis. Caspase-3 mRNA was significantly upregulated with E2 and miR-23a mimic treatment (P\0.0001 for E2, and P\0.01 for miR-23a mimic). Treatment of cells with anitmiR- 23a increased XIAP expression (P\0.001), and abolished caspase-3 activation (P\0.001). CONCLUSIONS: This study revealed a novel E2-signaling mechanism in regulating miRNAs expression and apoptosis that may contribute to sex difference in HCC development. |
Description | Conference Theme: New Horizons from East to west in Hepatology Topic 4: Basic Science of Hepatology: no. 2102 This journal suppl. entitled: Conference Abstracts: 24th Annual Conference of APASL, March 12-15, 2015, Istanbul, Turkey |
Persistent Identifier | http://hdl.handle.net/10722/214127 |
ISSN | 2023 Impact Factor: 5.9 2023 SCImago Journal Rankings: 1.813 |
DC Field | Value | Language |
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dc.contributor.author | Huang, FY | - |
dc.contributor.author | Wong, DKH | - |
dc.contributor.author | Seto, WK | - |
dc.contributor.author | Lai, CL | - |
dc.contributor.author | Yuen, MF | - |
dc.date.accessioned | 2015-08-21T03:08:36Z | - |
dc.date.available | 2015-08-21T03:08:36Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 24th Annual Conference of the Asian Pacific Association for the Study of the Liver (APASL 2015), Istanbul, Turkey, 12-15 March 2015. In Hepatology International, 2015, v. 9 suppl. 1, p. S137, abstract no. 2102-S138 | - |
dc.identifier.issn | 1936-0533 | - |
dc.identifier.uri | http://hdl.handle.net/10722/214127 | - |
dc.description | Conference Theme: New Horizons from East to west in Hepatology | - |
dc.description | Topic 4: Basic Science of Hepatology: no. 2102 | - |
dc.description | This journal suppl. entitled: Conference Abstracts: 24th Annual Conference of APASL, March 12-15, 2015, Istanbul, Turkey | - |
dc.description.abstract | BACKGROUND: Estrogen (E2) exerts a protective role against hepatocellular carcinoma (HCC) development. We sought to determine the effects of E2 on apoptotic miRNAs expression and explore the possible mechanism underlying apoptosis in HCC. METHODS: Microarray was performed to analyze alteration of apoptotic miRNAs in E2-treated liver cell-line. Expression profiles of selected miRNAs were verified using qRT-PCR. qRT-PCR and Western-blot were used to analyze the alteration of mRNA and protein levels of genes such as p53, ERa, XIAP and caspase-3/7. Activity of caspase3/7 was measured using a luminescent assay. RESULTS: After E2 treatment, more than twofold alteration was observed in 25 upregulated and 10 downregulated miRNAs. Expression of miR-23a in p53-mutated male cell-lines was significantly lower than cell-lines with functional p53 (all P\0.001), but not in female cells. p53 activation increased miR-23a expression in p53 +/+ HepG2 cells (P\0.0001 at 12 h. P\0.01 at 24 h), but not in p53-/-Hep3B cells. E2 via ERa could significantly activated miR- 23a (P\0.001) and p53 (P\0.01) expression, and thus replenished p53-deficiency SNU387 cells in activation of miR-23a. Moreover, miR-23a mimic and E2 significantly activated miR-23a (P\0.001) and suppressed the expression of its target XIAP (P\0.0001 and P\0.01). Decreasing of XIAP contributes to activation of caspase-3 activity and cell apoptosis. Caspase-3 mRNA was significantly upregulated with E2 and miR-23a mimic treatment (P\0.0001 for E2, and P\0.01 for miR-23a mimic). Treatment of cells with anitmiR- 23a increased XIAP expression (P\0.001), and abolished caspase-3 activation (P\0.001). CONCLUSIONS: This study revealed a novel E2-signaling mechanism in regulating miRNAs expression and apoptosis that may contribute to sex difference in HCC development. | - |
dc.language | eng | - |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0 | - |
dc.relation.ispartof | Hepatology International | - |
dc.rights | The final publication is available at Springer via http://dx.doi.org/10.1007/s12072-015-9609-1 | - |
dc.subject | Medical sciences | - |
dc.subject | Endocrinology | - |
dc.title | Estradiol activated microrna 23a directly or synergistically with p53 implicated in sex difference in hepatocellular carcinoma development | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Huang, FY: fungyu@hkucc.hku.hk | - |
dc.identifier.email | Wong, DKH: danywong@hku.hk | - |
dc.identifier.email | Seto, WK: wkseto@hku.hk | - |
dc.identifier.email | Lai, CL: hrmelcl@hkucc.hku.hk | - |
dc.identifier.email | Yuen, MF: mfyuen@hku.hk | - |
dc.identifier.authority | Wong, DKH=rp00492 | - |
dc.identifier.authority | Seto, WK=rp01659 | - |
dc.identifier.authority | Lai, CL=rp00314 | - |
dc.identifier.authority | Yuen, MF=rp00479 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s12072-015-9609-1 | - |
dc.identifier.scopus | eid_2-s2.0-85047596667 | - |
dc.identifier.hkuros | 248043 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | suppl. 1 | - |
dc.identifier.spage | S137, abstract no. 2102 | - |
dc.identifier.epage | S138 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1936-0533 | - |