File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Gallic acid-L-leucine conjugate corrects lipid mediator profiles in LPS-stimulated RAW264.7 via suppressing arachidonic acid metabolism
Title | Gallic acid-L-leucine conjugate corrects lipid mediator profiles in LPS-stimulated RAW264.7 via suppressing arachidonic acid metabolism |
---|---|
Authors | |
Issue Date | 2014 |
Citation | The 10th International Postgraduate Symposium on Chinese Medicine, Hong Kong, 15 August 2014. How to Cite? |
Abstract | Gallic acid-L-leucine conjugate (GL) is a synthesized compound by gallic acid and methyl-L-leucine. This study was designed to investigate the anti-inflammatory effect of GL on the generation of arachidonic acid (AA) pathway-derived inflammatory lipid mediators in RAW 264.7 cells. Based on the detection by LC/MS/MS, we found that GL profoundly suppressed LPS-induced release of AA metabolites such as prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), prostaglandin F2 (PGF2) and thromboxane B2 (TXB2). To characterize the molecular mechanisms underlying the actions of GL on pro-inflammatory lipid mediators, we first investigated LPS-induced expression of AA-metabolizing enzymes such as cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) as well as inducible NO synthase (iNOS). According to the results of Western blotting, GL decreased the expression of these three enzymes in a concentration-dependent manner. We further demonstrated that GL attenuated the LPS-induced activation of transcription factors NF-κB and AP-1. Along this line, we found GL prevented LPS-induced phosphorylation of p38, JNK and ERK1/2, and inhibited LPS-induced oxidative stress. Collectively, GL may inhibit LPS-induced inflammation via sequentially activating kinases JNK, p38 and ERK1/2, and transcription factors NF-κB and AP-1 in macrophages. Importantly, the results of this study indicate that the GL may exhibits its potential anti-inflammatory activity through correcting the profiles of AA-derived lipid mediators. |
Persistent Identifier | http://hdl.handle.net/10722/214855 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheng, Y | - |
dc.contributor.author | Tse, HF | - |
dc.contributor.author | Rong, J | - |
dc.date.accessioned | 2015-08-21T11:58:50Z | - |
dc.date.available | 2015-08-21T11:58:50Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | The 10th International Postgraduate Symposium on Chinese Medicine, Hong Kong, 15 August 2014. | - |
dc.identifier.uri | http://hdl.handle.net/10722/214855 | - |
dc.description.abstract | Gallic acid-L-leucine conjugate (GL) is a synthesized compound by gallic acid and methyl-L-leucine. This study was designed to investigate the anti-inflammatory effect of GL on the generation of arachidonic acid (AA) pathway-derived inflammatory lipid mediators in RAW 264.7 cells. Based on the detection by LC/MS/MS, we found that GL profoundly suppressed LPS-induced release of AA metabolites such as prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), prostaglandin F2 (PGF2) and thromboxane B2 (TXB2). To characterize the molecular mechanisms underlying the actions of GL on pro-inflammatory lipid mediators, we first investigated LPS-induced expression of AA-metabolizing enzymes such as cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) as well as inducible NO synthase (iNOS). According to the results of Western blotting, GL decreased the expression of these three enzymes in a concentration-dependent manner. We further demonstrated that GL attenuated the LPS-induced activation of transcription factors NF-κB and AP-1. Along this line, we found GL prevented LPS-induced phosphorylation of p38, JNK and ERK1/2, and inhibited LPS-induced oxidative stress. Collectively, GL may inhibit LPS-induced inflammation via sequentially activating kinases JNK, p38 and ERK1/2, and transcription factors NF-κB and AP-1 in macrophages. Importantly, the results of this study indicate that the GL may exhibits its potential anti-inflammatory activity through correcting the profiles of AA-derived lipid mediators. | - |
dc.language | eng | - |
dc.relation.ispartof | International Postgraduate Symposium on Chinese Medicine | - |
dc.relation.ispartof | 2014 第十屆國際研究生中醫藥研討會 | - |
dc.title | Gallic acid-L-leucine conjugate corrects lipid mediator profiles in LPS-stimulated RAW264.7 via suppressing arachidonic acid metabolism | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Tse, HF: hftse@hkucc.hku.hk | - |
dc.identifier.email | Rong, J: jrong@hku.hk | - |
dc.identifier.authority | Tse, HF=rp00428 | - |
dc.identifier.authority | Rong, J=rp00515 | - |
dc.identifier.hkuros | 247195 | - |