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- Publisher Website: 10.1007/s00253-014-5939-5
- Scopus: eid_2-s2.0-84916879430
- PMID: 25081552
- WOS: WOS:000345331700029
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Article: Potentially novel copper resistance genes in copper-enriched activated sludge revealed by metagenomic analysis
Title | Potentially novel copper resistance genes in copper-enriched activated sludge revealed by metagenomic analysis |
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Authors | |
Keywords | Copper Copper resistance Copper resistance gene High-throughput sequencing Metagenome |
Issue Date | 2014 |
Publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm |
Citation | Applied Microbiology and Biotechnology, 2014, v. 98 n. 24, p. 10255-10266 How to Cite? |
Abstract | In this study, we utilized the Illumina high-throughput metagenomic approach to investigate diversity and abundance of both microbial community and copper resistance genes (CuRGs) in activated sludge (AS) which was enriched under copper selective stress up to 800 mg/L. The raw datasets (~3.5 Gb for each sample, i.e., the copper-enriched AS and the control AS) were merged and normalized for the BLAST analyses against the SILVA SSU rRNA gene database and self-constructed copper resistance protein database (CuRD). Also, the raw metagenomic sequences were assembled into contigs and analyzed based on Open Reading Frames (ORFs) to identify potentially novel copper resistance genes. Among the different resistance systems for copper detoxification under the high copper stress condition, the Cus system was the most enriched system. The results also indicated that genes encoding multi-copper oxidase played a more important role than those encoding efflux proteins. More significantly, several potentially novel copper resistance ORFs were identified by Pfam search and phylogenic analysis. This study demonstrated a new understanding of microbial-mediated copper resistance under high copper stress using high-throughput shotgun sequencing technique. |
Persistent Identifier | http://hdl.handle.net/10722/215201 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 0.957 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, L | - |
dc.contributor.author | Cai, L | - |
dc.contributor.author | Zhang, X | - |
dc.contributor.author | Zhang, T | - |
dc.date.accessioned | 2015-08-21T13:17:48Z | - |
dc.date.available | 2015-08-21T13:17:48Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Applied Microbiology and Biotechnology, 2014, v. 98 n. 24, p. 10255-10266 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10722/215201 | - |
dc.description.abstract | In this study, we utilized the Illumina high-throughput metagenomic approach to investigate diversity and abundance of both microbial community and copper resistance genes (CuRGs) in activated sludge (AS) which was enriched under copper selective stress up to 800 mg/L. The raw datasets (~3.5 Gb for each sample, i.e., the copper-enriched AS and the control AS) were merged and normalized for the BLAST analyses against the SILVA SSU rRNA gene database and self-constructed copper resistance protein database (CuRD). Also, the raw metagenomic sequences were assembled into contigs and analyzed based on Open Reading Frames (ORFs) to identify potentially novel copper resistance genes. Among the different resistance systems for copper detoxification under the high copper stress condition, the Cus system was the most enriched system. The results also indicated that genes encoding multi-copper oxidase played a more important role than those encoding efflux proteins. More significantly, several potentially novel copper resistance ORFs were identified by Pfam search and phylogenic analysis. This study demonstrated a new understanding of microbial-mediated copper resistance under high copper stress using high-throughput shotgun sequencing technique. | - |
dc.language | eng | - |
dc.publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | - |
dc.relation.ispartof | Applied Microbiology and Biotechnology | - |
dc.subject | Copper | - |
dc.subject | Copper resistance | - |
dc.subject | Copper resistance gene | - |
dc.subject | High-throughput sequencing | - |
dc.subject | Metagenome | - |
dc.title | Potentially novel copper resistance genes in copper-enriched activated sludge revealed by metagenomic analysis | - |
dc.type | Article | - |
dc.identifier.email | Li, L: liliguan@hku.hk | - |
dc.identifier.email | Zhang, T: zhangt@hkucc.hku.hk | - |
dc.identifier.authority | Li, L=rp02879 | - |
dc.identifier.authority | Zhang, T=rp00211 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00253-014-5939-5 | - |
dc.identifier.pmid | 25081552 | - |
dc.identifier.scopus | eid_2-s2.0-84916879430 | - |
dc.identifier.hkuros | 249666 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | 24 | - |
dc.identifier.spage | 10255 | - |
dc.identifier.epage | 10266 | - |
dc.identifier.eissn | 1432-0614 | - |
dc.identifier.isi | WOS:000345331700029 | - |
dc.publisher.place | Germany | - |
dc.identifier.issnl | 0175-7598 | - |