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- Publisher Website: 10.1016/j.cub.2013.04.057
- Scopus: eid_2-s2.0-84879286502
- PMID: 23746641
- WOS: WOS:000320682800017
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Article: Dynamic localization of G-actin during membrane protrusion in neuronal motility
Title | Dynamic localization of G-actin during membrane protrusion in neuronal motility |
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Authors | |
Issue Date | 2013 |
Citation | Current Biology, 2013, v. 23, n. 12, p. 1046-1056 How to Cite? |
Abstract | Background Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility. Results Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusions. Moreover, G-actin localization occurs asymmetrically in growth cones during attractive turning. Finally, we identify the actin monomer-binding proteins profilin and thymosin β4 as key molecules that localize actin monomers to the leading edge of lamellipodia for their motility. Conclusions Our results suggest that dynamic localization of G-actin provides a novel mechanism to regulate the spatiotemporal actin dynamics underlying membrane protrusion in cell locomotion and growth cone chemotaxis. © 2013 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/219881 |
ISSN | 2023 Impact Factor: 8.1 2023 SCImago Journal Rankings: 2.982 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lee, Chi Wai | - |
dc.contributor.author | Vitriol, Eric A. | - |
dc.contributor.author | Shim, Sangwoo | - |
dc.contributor.author | Wise, Ariel L. | - |
dc.contributor.author | Velayutham, Radhi P. | - |
dc.contributor.author | Zheng, James Q. | - |
dc.date.accessioned | 2015-09-24T04:44:15Z | - |
dc.date.available | 2015-09-24T04:44:15Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Current Biology, 2013, v. 23, n. 12, p. 1046-1056 | - |
dc.identifier.issn | 0960-9822 | - |
dc.identifier.uri | http://hdl.handle.net/10722/219881 | - |
dc.description.abstract | Background Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility. Results Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusions. Moreover, G-actin localization occurs asymmetrically in growth cones during attractive turning. Finally, we identify the actin monomer-binding proteins profilin and thymosin β4 as key molecules that localize actin monomers to the leading edge of lamellipodia for their motility. Conclusions Our results suggest that dynamic localization of G-actin provides a novel mechanism to regulate the spatiotemporal actin dynamics underlying membrane protrusion in cell locomotion and growth cone chemotaxis. © 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Current Biology | - |
dc.title | Dynamic localization of G-actin during membrane protrusion in neuronal motility | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/j.cub.2013.04.057 | - |
dc.identifier.pmid | 23746641 | - |
dc.identifier.scopus | eid_2-s2.0-84879286502 | - |
dc.identifier.volume | 23 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1046 | - |
dc.identifier.epage | 1056 | - |
dc.identifier.isi | WOS:000320682800017 | - |
dc.identifier.issnl | 0960-9822 | - |