Auto-antibody to Koc in patients with cancers

Abstract

Based on screening conditions on Koc (KH domain containing protein overexpressed in cancer) expression and purification, recombinant Koc was used as coating antigen for establishment of ELISA to detect anti-Koc of classes of IgM, IgG and IgA. The positive rates of anti-Koc were related to types of cancers, showing higher rates in hepatocellular cancer, rectum cancer and lung cancer, middle rates in stomach cancer, and lower rates in esophagus cancer. The last one was not significant, compared with normal control. When positive samples were defined as being positive for anti-Koc in at least one of three classes of Ig, the positive rates of hepatocellular cancer, rectum cancer, lung cancer, stomach cancer, esophagus cancer were 56.7%, 52.8%, 50.0%, 25.5% and 15.8%, respectively. Their positive rates were significantly higher than that of IgM, IgG or IgA anti-Koc in these cancers.