File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1371/journal.pone.0062885
- Scopus: eid_2-s2.0-84876740446
- PMID: 23638162
- WOS: WOS:000318341400073
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis - A Longitudinal Study
Title | Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis - A Longitudinal Study |
---|---|
Authors | |
Issue Date | 2013 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | PLOS One, 2013, v. 8 n. 4, p. e62885 How to Cite? |
Abstract | BACKGROUND:
The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied.
METHODS:
Fecal microbiota of histology-proven NASH patients and healthy controls was analyzed by 16S ribosomal RNA pyrosequencing. NASH patients were from a previously reported randomized trial on probiotic treatment. Proton-magnetic resonance spectroscopy was performed to monitor changes in intrahepatic triglyceride content (IHTG).
RESULTS:
A total of 420,344 16S sequences with acceptable quality were obtained from 16 NASH patients and 22 controls. NASH patients had lower fecal abundance of Faecalibacterium and Anaerosporobacter but higher abundance of Parabacteroides and Allisonella. Partial least-square discriminant analysis yielded a model of 10 genera that discriminated NASH patients from controls. At month 6, 6 of 7 patients in the probiotic group and 4 of 9 patients in the usual care group had improvement in IHTG (P=0.15). Improvement in IHTG was associated with a reduction in the abundance of Firmicutes (R(2)=0.4820, P=0.0028) and increase in Bacteroidetes (R(2)=0.4366, P=0.0053). This was accompanied by corresponding changes at the class, order and genus levels. In contrast, bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment.
CONCLUSIONS:
NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis. Further studies are required to investigate the mechanism underlying the interaction between gut microbes and the liver. |
Persistent Identifier | http://hdl.handle.net/10722/221829 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.839 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, VWS | - |
dc.contributor.author | Tse, CH | - |
dc.contributor.author | Lam, TY | - |
dc.contributor.author | Wong, GLH | - |
dc.contributor.author | Chim, AML | - |
dc.contributor.author | Chu, WCW | - |
dc.contributor.author | Yeung, DKW | - |
dc.contributor.author | Law, PTW | - |
dc.contributor.author | Kwan, HS | - |
dc.contributor.author | Yu, J | - |
dc.contributor.author | Sung, JJY | - |
dc.contributor.author | Chan, HLY | - |
dc.date.accessioned | 2015-12-10T04:22:00Z | - |
dc.date.available | 2015-12-10T04:22:00Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLOS One, 2013, v. 8 n. 4, p. e62885 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10722/221829 | - |
dc.description.abstract | BACKGROUND: The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. METHODS: Fecal microbiota of histology-proven NASH patients and healthy controls was analyzed by 16S ribosomal RNA pyrosequencing. NASH patients were from a previously reported randomized trial on probiotic treatment. Proton-magnetic resonance spectroscopy was performed to monitor changes in intrahepatic triglyceride content (IHTG). RESULTS: A total of 420,344 16S sequences with acceptable quality were obtained from 16 NASH patients and 22 controls. NASH patients had lower fecal abundance of Faecalibacterium and Anaerosporobacter but higher abundance of Parabacteroides and Allisonella. Partial least-square discriminant analysis yielded a model of 10 genera that discriminated NASH patients from controls. At month 6, 6 of 7 patients in the probiotic group and 4 of 9 patients in the usual care group had improvement in IHTG (P=0.15). Improvement in IHTG was associated with a reduction in the abundance of Firmicutes (R(2)=0.4820, P=0.0028) and increase in Bacteroidetes (R(2)=0.4366, P=0.0053). This was accompanied by corresponding changes at the class, order and genus levels. In contrast, bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment. CONCLUSIONS: NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis. Further studies are required to investigate the mechanism underlying the interaction between gut microbes and the liver. | - |
dc.language | eng | - |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | - |
dc.relation.ispartof | PLoS ONE | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis - A Longitudinal Study | - |
dc.type | Article | - |
dc.identifier.email | Lam, TY: ttylam@hku.hk | - |
dc.identifier.authority | Lam, TY=rp01733 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0062885 | - |
dc.identifier.pmid | 23638162 | - |
dc.identifier.pmcid | PMC3636208 | - |
dc.identifier.scopus | eid_2-s2.0-84876740446 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | e62885 | - |
dc.identifier.epage | e62885 | - |
dc.identifier.isi | WOS:000318341400073 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1932-6203 | - |