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Article: HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay

TitleHKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay
Authors
Issue Date2016
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp
Citation
Chemical Science, 2016, v. 7 n. 3, p. 2094-2099 How to Cite?
AbstractUltra-selective and ultra-sensitive probes for hypochlorous acid (HOCl), one of the most poorly understood reactive oxygen species (ROS), are urgently needed to unravel the HOCl functions in important biological processes such as development and innate immunity. Based on a selective oxidative O-dearylation reaction of 2,6-dichlorophenol toward HOCl over other reactive oxygen species, we have developed a novel fluorescent probe HKOCl-3 for HOCl detection with ultra-selectivity, ultra-sensitivity and a rapid turn-on response. The functional robustness of HKOCl-3 for endogenous HOCl detection and imaging has been thoroughly scrutinized in multiple types of phagocytes and in vivo imaging of live intact zebrafish embryos. Furthermore, HKOCl-3 has been successfully applied to the detection of endogenous HOCl by a 96-well microplate assay and flow cytometry. Therefore, HKOCl-3 holds great promise as a versatile molecular tool that enables innovative investigation of HOCl biology and ROS-related diseases in multiple detection modalities.
Persistent Identifierhttp://hdl.handle.net/10722/231635
ISSN
2023 Impact Factor: 7.6
2023 SCImago Journal Rankings: 2.333
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHu, J-
dc.contributor.authorWong, NK-
dc.contributor.authorLu, M-
dc.contributor.authorChen, X-
dc.contributor.authorYe, S-
dc.contributor.authorZhao, Q-
dc.contributor.authorGao, P-
dc.contributor.authorKao, RYT-
dc.contributor.authorShen, J-
dc.contributor.authorYang, D-
dc.date.accessioned2016-09-20T05:24:31Z-
dc.date.available2016-09-20T05:24:31Z-
dc.date.issued2016-
dc.identifier.citationChemical Science, 2016, v. 7 n. 3, p. 2094-2099-
dc.identifier.issn2041-6520-
dc.identifier.urihttp://hdl.handle.net/10722/231635-
dc.description.abstractUltra-selective and ultra-sensitive probes for hypochlorous acid (HOCl), one of the most poorly understood reactive oxygen species (ROS), are urgently needed to unravel the HOCl functions in important biological processes such as development and innate immunity. Based on a selective oxidative O-dearylation reaction of 2,6-dichlorophenol toward HOCl over other reactive oxygen species, we have developed a novel fluorescent probe HKOCl-3 for HOCl detection with ultra-selectivity, ultra-sensitivity and a rapid turn-on response. The functional robustness of HKOCl-3 for endogenous HOCl detection and imaging has been thoroughly scrutinized in multiple types of phagocytes and in vivo imaging of live intact zebrafish embryos. Furthermore, HKOCl-3 has been successfully applied to the detection of endogenous HOCl by a 96-well microplate assay and flow cytometry. Therefore, HKOCl-3 holds great promise as a versatile molecular tool that enables innovative investigation of HOCl biology and ROS-related diseases in multiple detection modalities.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp-
dc.relation.ispartofChemical Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleHKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay-
dc.typeArticle-
dc.identifier.emailHu, J: hujun@hku.hk-
dc.identifier.emailChen, X: chenxm@hku.hk-
dc.identifier.emailZhao, Q: angelaqz@hku.hk-
dc.identifier.emailGao, P: gaopeng@hku.hk-
dc.identifier.emailKao, RYT: rytkao@hkucc.hku.hk-
dc.identifier.emailShen, J: shenjg@hku.hk-
dc.identifier.emailYang, D: yangdan@hku.hk-
dc.identifier.authorityKao, RYT=rp00481-
dc.identifier.authorityShen, J=rp00487-
dc.identifier.authorityYang, D=rp00825-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1039/C5SC03855C-
dc.identifier.pmid29899935-
dc.identifier.pmcidPMC5968534-
dc.identifier.scopuseid_2-s2.0-84959353893-
dc.identifier.hkuros263076-
dc.identifier.volume7-
dc.identifier.issue3-
dc.identifier.spage2094-
dc.identifier.epage2099-
dc.identifier.isiWOS:000371021900057-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2041-6520-

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