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- Publisher Website: 10.1007/s10867-016-9423-6
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Article: Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy
Title | Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy |
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Authors | |
Keywords | Atomic force microscopy Cell Methotrexate Relaxation time Viscoelastic properties Young’s modulus |
Issue Date | 2016 |
Publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0092-0606 |
Citation | Journal of Biological Physics, 2016, v. 42 n. 4, p. 551-569 How to Cite? |
Abstract | Methotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation. |
Persistent Identifier | http://hdl.handle.net/10722/234597 |
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.323 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LI, M | - |
dc.contributor.author | LIU, L | - |
dc.contributor.author | XIAO, X | - |
dc.contributor.author | Xi, N | - |
dc.contributor.author | WANG, Y | - |
dc.date.accessioned | 2016-10-14T13:47:56Z | - |
dc.date.available | 2016-10-14T13:47:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Journal of Biological Physics, 2016, v. 42 n. 4, p. 551-569 | - |
dc.identifier.issn | 0092-0606 | - |
dc.identifier.uri | http://hdl.handle.net/10722/234597 | - |
dc.description.abstract | Methotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation. | - |
dc.language | eng | - |
dc.publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0092-0606 | - |
dc.relation.ispartof | Journal of Biological Physics | - |
dc.rights | The final publication is available at Springer via http://dx.doi.org/[insert DOI] | - |
dc.subject | Atomic force microscopy | - |
dc.subject | Cell | - |
dc.subject | Methotrexate | - |
dc.subject | Relaxation time | - |
dc.subject | Viscoelastic properties | - |
dc.subject | Young’s modulus | - |
dc.title | Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy | - |
dc.type | Article | - |
dc.identifier.email | Xi, N: xining@hku.hk | - |
dc.identifier.authority | Xi, N=rp02044 | - |
dc.identifier.doi | 10.1007/s10867-016-9423-6 | - |
dc.identifier.scopus | eid_2-s2.0-84979294042 | - |
dc.identifier.hkuros | 269167 | - |
dc.identifier.volume | 42 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 551 | - |
dc.identifier.epage | 569 | - |
dc.identifier.isi | WOS:000385191200004 | - |
dc.publisher.place | Netherlands | - |
dc.identifier.issnl | 0092-0606 | - |