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Article: RbAp46/48LIN-53 is Required for Holocentromere Assembly in Caenorhabditis elegans
Title | RbAp46/48<font size=-1><sup>LIN-53</sup></font> is Required for Holocentromere Assembly in Caenorhabditis elegans |
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Authors | |
Issue Date | 2016 |
Publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports |
Citation | Cell Reports, 2016, v. 14 n. 8, p. 1819-1828 How to Cite? |
Abstract | Centromeres, the specialized chromosomal regions for recruiting kinetochores and directing chromosome segregation, are epigenetically marked by a centromeric histone H3 variant, CENP-A. To maintain centromere identity through cell cycles, CENP-A diluted during DNA replication is replenished. The licensing factor M18BP1KNL-2 is known to recruit CENP-A to holocentromeres. Here, we show that RbAp46/48LIN-53, a conserved histone chaperone, is required for CENP-AHCP-3 localization in holocentric Caenorhabditis elegans. Indeed, RbAp46/48LIN-53 and CENP-AHCP-3 localizations are interdependent. RbAp46/48LIN-53 localizes to the centromere during metaphase in a CENP-AHCP-3- and M18BP1KNL-2-dependent manner, suggesting CENP-AHCP-3 loading may occur before anaphase. RbAp46/48LIN-53 does not function at the centromere through histone acetylation, H3K27 trimethylation, or its known chromatin-modifying complexes. RbAp46/48LIN-53 may function independently to escort CENP-AHCP-3 for holocentromere assembly but is dispensable for other kinetochore protein recruitment. Nonetheless, depletion of RbAp46/48LIN-53 leads to anaphase bridges and chromosome missegregation. This study unravels the holocentromere assembly hierarchy and its conservation with monocentromeres. |
Persistent Identifier | http://hdl.handle.net/10722/235354 |
ISSN | 2023 Impact Factor: 7.5 2023 SCImago Journal Rankings: 4.279 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LEE, CH | - |
dc.contributor.author | LIN, Z | - |
dc.contributor.author | Yuen, KWY | - |
dc.date.accessioned | 2016-10-14T13:52:45Z | - |
dc.date.available | 2016-10-14T13:52:45Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Cell Reports, 2016, v. 14 n. 8, p. 1819-1828 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/10722/235354 | - |
dc.description.abstract | Centromeres, the specialized chromosomal regions for recruiting kinetochores and directing chromosome segregation, are epigenetically marked by a centromeric histone H3 variant, CENP-A. To maintain centromere identity through cell cycles, CENP-A diluted during DNA replication is replenished. The licensing factor M18BP1KNL-2 is known to recruit CENP-A to holocentromeres. Here, we show that RbAp46/48LIN-53, a conserved histone chaperone, is required for CENP-AHCP-3 localization in holocentric Caenorhabditis elegans. Indeed, RbAp46/48LIN-53 and CENP-AHCP-3 localizations are interdependent. RbAp46/48LIN-53 localizes to the centromere during metaphase in a CENP-AHCP-3- and M18BP1KNL-2-dependent manner, suggesting CENP-AHCP-3 loading may occur before anaphase. RbAp46/48LIN-53 does not function at the centromere through histone acetylation, H3K27 trimethylation, or its known chromatin-modifying complexes. RbAp46/48LIN-53 may function independently to escort CENP-AHCP-3 for holocentromere assembly but is dispensable for other kinetochore protein recruitment. Nonetheless, depletion of RbAp46/48LIN-53 leads to anaphase bridges and chromosome missegregation. This study unravels the holocentromere assembly hierarchy and its conservation with monocentromeres. | - |
dc.language | eng | - |
dc.publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports | - |
dc.relation.ispartof | Cell Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | RbAp46/48<font size=-1><sup>LIN-53</sup></font> is Required for Holocentromere Assembly in Caenorhabditis elegans | - |
dc.type | Article | - |
dc.identifier.email | Yuen, KWY: kwyyuen@hku.hk | - |
dc.identifier.authority | Yuen, KWY=rp01512 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.celrep.2016.01.065 | - |
dc.identifier.scopus | eid_2-s2.0-84959238490 | - |
dc.identifier.hkuros | 268840 | - |
dc.identifier.volume | 14 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 1819 | - |
dc.identifier.epage | 1828 | - |
dc.identifier.isi | WOS:000371217700002 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2211-1247 | - |