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- Publisher Website: 10.1016/j.pdpdt.2014.10.011
- Scopus: eid_2-s2.0-84924801525
- PMID: 25462576
- WOS: WOS:000351193300014
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Article: Photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin
Title | Photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin |
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Authors | |
Keywords | Curcumin Confocal microscopy Biofilm Photosensitizer Sodium hypochlorite Enterococcus faecalis |
Issue Date | 2015 |
Citation | Photodiagnosis and Photodynamic Therapy, 2015, v. 12, n. 1, p. 108-114 How to Cite? |
Abstract | © 2014 Elsevier B.V.Background: To test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against Enterococcus faecalis biofilms in vitro. Methods: E. faecalis biofilms were grown within root canals (n=175) and divided into 7 groups (n=25). Group 1, sterile saline; group 2, 3% sodium hypochlorite; group 3, 3% sodium hypochlorite activated with ultrasonic files (30s cycles for 4min); group 4, 3% sodium hypochlorite irradiated with blue light (1200mw/cm2 for 4min); group 5, curcumin (2.5mg/mL); group 6, curcumin (2.5mg/mL) activated with ultrasonic files (30s cycles for 4min); group 7, curcumin (2.5mg/mL) irradiated with blue light. The biofilms' ultrastructure was examined using scanning electron microscopy. Bacterial viability was assessed by confocal microscopy. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test (P=0.05). The quantitative analysis of the colony-forming units was carried out from dentinal shaving and analyzed by One-way ANOVA and Tukey multiple comparison test (P=0.05). Results: All treatment groups showed a significantly higher percentage of dead bacteria than the saline control (P. <. 0.05). The percentage of dead bacteria was significantly higher when light activated curcumin was used (P. <. 0.05). At both depths (200 and 400 microns), light activated curcumin showed no growth of bacteria. Conclusions: Light activation produced significantly higher antibacterial efficacy than ultrasonic agitation, with light activated curcumin producing the maximum elimination of biofilm bacteria within the root canal lumen and dentinal tubules. |
Persistent Identifier | http://hdl.handle.net/10722/236052 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.718 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Neelakantan, Prasanna | - |
dc.contributor.author | Cheng, Cheng Qing | - |
dc.contributor.author | Ravichandran, Vinoddhine | - |
dc.contributor.author | Mao, Teresa | - |
dc.contributor.author | Sriraman, Priyanka | - |
dc.contributor.author | Sridharan, Swetha | - |
dc.contributor.author | Subbarao, Chandana | - |
dc.contributor.author | Sharma, Subash | - |
dc.contributor.author | Kishen, Anil | - |
dc.date.accessioned | 2016-11-10T07:12:04Z | - |
dc.date.available | 2016-11-10T07:12:04Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Photodiagnosis and Photodynamic Therapy, 2015, v. 12, n. 1, p. 108-114 | - |
dc.identifier.issn | 1572-1000 | - |
dc.identifier.uri | http://hdl.handle.net/10722/236052 | - |
dc.description.abstract | © 2014 Elsevier B.V.Background: To test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against Enterococcus faecalis biofilms in vitro. Methods: E. faecalis biofilms were grown within root canals (n=175) and divided into 7 groups (n=25). Group 1, sterile saline; group 2, 3% sodium hypochlorite; group 3, 3% sodium hypochlorite activated with ultrasonic files (30s cycles for 4min); group 4, 3% sodium hypochlorite irradiated with blue light (1200mw/cm2 for 4min); group 5, curcumin (2.5mg/mL); group 6, curcumin (2.5mg/mL) activated with ultrasonic files (30s cycles for 4min); group 7, curcumin (2.5mg/mL) irradiated with blue light. The biofilms' ultrastructure was examined using scanning electron microscopy. Bacterial viability was assessed by confocal microscopy. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test (P=0.05). The quantitative analysis of the colony-forming units was carried out from dentinal shaving and analyzed by One-way ANOVA and Tukey multiple comparison test (P=0.05). Results: All treatment groups showed a significantly higher percentage of dead bacteria than the saline control (P. <. 0.05). The percentage of dead bacteria was significantly higher when light activated curcumin was used (P. <. 0.05). At both depths (200 and 400 microns), light activated curcumin showed no growth of bacteria. Conclusions: Light activation produced significantly higher antibacterial efficacy than ultrasonic agitation, with light activated curcumin producing the maximum elimination of biofilm bacteria within the root canal lumen and dentinal tubules. | - |
dc.language | eng | - |
dc.relation.ispartof | Photodiagnosis and Photodynamic Therapy | - |
dc.subject | Curcumin | - |
dc.subject | Confocal microscopy | - |
dc.subject | Biofilm | - |
dc.subject | Photosensitizer | - |
dc.subject | Sodium hypochlorite | - |
dc.subject | Enterococcus faecalis | - |
dc.title | Photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.pdpdt.2014.10.011 | - |
dc.identifier.pmid | 25462576 | - |
dc.identifier.scopus | eid_2-s2.0-84924801525 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 108 | - |
dc.identifier.epage | 114 | - |
dc.identifier.eissn | 1873-1597 | - |
dc.identifier.isi | WOS:000351193300014 | - |
dc.identifier.issnl | 1572-1000 | - |