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Article: Reprogramming of Dermal Fibroblasts into Osteo-Chondrogenic Cells with Elevated Osteogenic Potency by Defined Transcription Factors
Title | Reprogramming of Dermal Fibroblasts into Osteo-Chondrogenic Cells with Elevated Osteogenic Potency by Defined Transcription Factors |
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Authors | |
Keywords | c-MYC chondrocytes KLF4 lentiviruses limb bud osteo-chondroprogenitors osteoblasts reprogramming RUNX2 SOX9 |
Issue Date | 2017 |
Publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at http://stemcellreports.cell.com |
Citation | Stem Cell Reports, 2017, v. 8 n. 6, p. 1587-1599 How to Cite? |
Abstract | Recent studies using defined transcription factors to convert skin fibroblasts into chondrocytes have raised the question of whether osteo-chondroprogenitors expressing SOX9 and RUNX2 could also be generated during the course of the reprogramming process. Here, we demonstrated that doxycycline-inducible expression of reprogramming factors (KLF4 [K] and c-MYC [M]) for 6 days were sufficient to convert murine fibroblasts into SOX9+/RUNX2+ cellular aggregates and together with SOX9 (S) promoted the conversion efficiency when cultured in a defined stem cell medium, mTeSR. KMS-reprogrammed cells possess gene expression profiles akin to those of native osteo-chondroprogenitors with elevated osteogenic properties and can differentiate into osteoblasts and chondrocytes in vitro, but form bone tissue upon transplantation under the skin and in the fracture site of mouse tibia. Altogether, we provide a reprogramming strategy to enable efficient derivation of osteo-chondrogenic cells that may hold promise for cell replacement therapy not limited to cartilage but also for bone tissues. |
Persistent Identifier | http://hdl.handle.net/10722/241531 |
ISSN | 2023 Impact Factor: 5.9 2023 SCImago Journal Rankings: 2.518 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, Y | - |
dc.contributor.author | Wu, MH | - |
dc.contributor.author | Cheung, MPL | - |
dc.contributor.author | Sham, MH | - |
dc.contributor.author | Haruhiko, A | - |
dc.contributor.author | Chan, D | - |
dc.contributor.author | Cheah, KSE | - |
dc.contributor.author | Cheung, MCH | - |
dc.date.accessioned | 2017-06-20T01:44:59Z | - |
dc.date.available | 2017-06-20T01:44:59Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Stem Cell Reports, 2017, v. 8 n. 6, p. 1587-1599 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/10722/241531 | - |
dc.description.abstract | Recent studies using defined transcription factors to convert skin fibroblasts into chondrocytes have raised the question of whether osteo-chondroprogenitors expressing SOX9 and RUNX2 could also be generated during the course of the reprogramming process. Here, we demonstrated that doxycycline-inducible expression of reprogramming factors (KLF4 [K] and c-MYC [M]) for 6 days were sufficient to convert murine fibroblasts into SOX9+/RUNX2+ cellular aggregates and together with SOX9 (S) promoted the conversion efficiency when cultured in a defined stem cell medium, mTeSR. KMS-reprogrammed cells possess gene expression profiles akin to those of native osteo-chondroprogenitors with elevated osteogenic properties and can differentiate into osteoblasts and chondrocytes in vitro, but form bone tissue upon transplantation under the skin and in the fracture site of mouse tibia. Altogether, we provide a reprogramming strategy to enable efficient derivation of osteo-chondrogenic cells that may hold promise for cell replacement therapy not limited to cartilage but also for bone tissues. | - |
dc.language | eng | - |
dc.publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at http://stemcellreports.cell.com | - |
dc.relation.ispartof | Stem Cell Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | c-MYC | - |
dc.subject | chondrocytes | - |
dc.subject | KLF4 | - |
dc.subject | lentiviruses | - |
dc.subject | limb bud | - |
dc.subject | osteo-chondroprogenitors | - |
dc.subject | osteoblasts | - |
dc.subject | reprogramming | - |
dc.subject | RUNX2 | - |
dc.subject | SOX9 | - |
dc.title | Reprogramming of Dermal Fibroblasts into Osteo-Chondrogenic Cells with Elevated Osteogenic Potency by Defined Transcription Factors | - |
dc.type | Article | - |
dc.identifier.email | Wang, Y: h0894106@hku.hk | - |
dc.identifier.email | Wu, MH: ronmhwu@hkucc.hku.hk | - |
dc.identifier.email | Cheung, MPL: mplcheun@hku.hk | - |
dc.identifier.email | Sham, MH: mhsham@hku.hk | - |
dc.identifier.email | Chan, D: chand@hku.hk | - |
dc.identifier.email | Cheah, KSE: hrmbdkc@hku.hk | - |
dc.identifier.email | Cheung, MCH: mcheung9@hku.hk | - |
dc.identifier.authority | Sham, MH=rp00380 | - |
dc.identifier.authority | Chan, D=rp00540 | - |
dc.identifier.authority | Cheah, KSE=rp00342 | - |
dc.identifier.authority | Cheung, MCH=rp00245 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.stemcr.2017.04.018 | - |
dc.identifier.scopus | eid_2-s2.0-85019355132 | - |
dc.identifier.hkuros | 272651 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1587 | - |
dc.identifier.epage | 1599 | - |
dc.identifier.isi | WOS:000402964700015 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2213-6711 | - |