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Article: Maturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation

TitleMaturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation
Authors
KeywordsHeart tissue engineering
Maturation
Human embryonic stem cell
Mechanical loading
Mesenchymal cell
Issue Date2017
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2017, v. 49, p. 204-217 How to Cite?
AbstractCardiomyocytes derived from human embryonic stem cells (hESC-CMs) are regarded as a promising source for regenerative medicine, drug testing and disease modeling. Nevertheless, cardiomyocytes are immature in terms of their contractile structure, metabolism and electrophysiological properties. Here, we fabricate cardiac muscle strips by encapsulating hESC-CMs in collagen-based biomaterials. Supplementation of niche cells at 3% to the number of hESC-CMs enhance the maturation of the hESC-CMs in 3D tissue matrix. The benefits of adding mesenchymal stem cells (MSCs) are comparable to that of adding fibroblasts. These two cell types demonstrate similar effects in promoting the compaction and cell spreading, as well as expression of maturation markers at both gene and protein levels. Mechanical loading, particularly cyclic stretch, produces engineered cardiac tissues with higher maturity in terms of twitch force, elastic modulus, sarcomere length and molecular signature, when comparing to static stretch or non-stretched controls. The current study demonstrates that the application of niche cells and mechanical stretch both stimulate the maturation of hESC-CMs in 3D architecture. Our results therefore suggest that this 3D model can be used for in vitro cardiac maturation study.
Persistent Identifierhttp://hdl.handle.net/10722/244663
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 1.925
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, W-
dc.contributor.authorKong, CW-
dc.contributor.authorTong, MH-
dc.contributor.authorChooi, WH-
dc.contributor.authorHuang, N-
dc.contributor.authorLi, RA-
dc.contributor.authorChan, BP-
dc.date.accessioned2017-09-18T01:56:46Z-
dc.date.available2017-09-18T01:56:46Z-
dc.date.issued2017-
dc.identifier.citationActa Biomaterialia, 2017, v. 49, p. 204-217-
dc.identifier.issn1742-7061-
dc.identifier.urihttp://hdl.handle.net/10722/244663-
dc.description.abstractCardiomyocytes derived from human embryonic stem cells (hESC-CMs) are regarded as a promising source for regenerative medicine, drug testing and disease modeling. Nevertheless, cardiomyocytes are immature in terms of their contractile structure, metabolism and electrophysiological properties. Here, we fabricate cardiac muscle strips by encapsulating hESC-CMs in collagen-based biomaterials. Supplementation of niche cells at 3% to the number of hESC-CMs enhance the maturation of the hESC-CMs in 3D tissue matrix. The benefits of adding mesenchymal stem cells (MSCs) are comparable to that of adding fibroblasts. These two cell types demonstrate similar effects in promoting the compaction and cell spreading, as well as expression of maturation markers at both gene and protein levels. Mechanical loading, particularly cyclic stretch, produces engineered cardiac tissues with higher maturity in terms of twitch force, elastic modulus, sarcomere length and molecular signature, when comparing to static stretch or non-stretched controls. The current study demonstrates that the application of niche cells and mechanical stretch both stimulate the maturation of hESC-CMs in 3D architecture. Our results therefore suggest that this 3D model can be used for in vitro cardiac maturation study.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat-
dc.relation.ispartofActa Biomaterialia-
dc.subjectHeart tissue engineering-
dc.subjectMaturation-
dc.subjectHuman embryonic stem cell-
dc.subjectMechanical loading-
dc.subjectMesenchymal cell-
dc.titleMaturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation-
dc.typeArticle-
dc.identifier.emailKong, CW: mkcwkong@hku.hk-
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hk-
dc.identifier.emailChan, BP: bpchan@hku.hk-
dc.identifier.authorityKong, CW=rp01563-
dc.identifier.authorityLi, RA=rp01352-
dc.identifier.authorityChan, BP=rp00087-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.actbio.2016.11.058-
dc.identifier.pmid27890729-
dc.identifier.scopuseid_2-s2.0-85007482766-
dc.identifier.hkuros277599-
dc.identifier.volume49-
dc.identifier.spage204-
dc.identifier.epage217-
dc.identifier.isiWOS:000394062100016-
dc.publisher.placeNetherlands-
dc.identifier.issnl1742-7061-

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