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Conference Paper: Synthetic KL4 peptide as new carrier of siRNA therapeutics for pulmonary delivery
Title | Synthetic KL4 peptide as new carrier of siRNA therapeutics for pulmonary delivery |
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Authors | |
Issue Date | 2017 |
Publisher | Mary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/products/product.aspx?pid=24 |
Citation | The 27th Drug Delivery to the Lungs (DDL27) Conference, Edinburgh, Scotland, UK, 7-9 December 2016. In Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2017, v. 30 n. 4, p. A-11, abstract no. 33 How to Cite? |
Abstract | Small interfering RNA (siRNA) has great potential for the treatment of various respiratory diseases through RNA interference (RNAi), but their clinical application is hindered by the lack of a safe and effective pulmonary delivery system. KL4 peptide is a synthetic amphipathic peptide that was previously developed to mimic the function of pulmonary surfactant protein B (SP-B). Its potential as siRNA carrier for pulmonary delivery was examined in this study. The cationic KL4 peptide was able to bind with siRNA to form complexes at 15:1 ratio (peptide to siRNA weight ratio) or above. It also mediated efficient gene silencing on lung epithelial cells, with 20:1 ratio as the optimal ratio for siRNA transfection. Furthermore, the KL4/siRNA complexes were not toxic at concentrations used for transfection in vitro. The study shows that KL4 peptide appears to be a promising candidate for siRNA delivery. Further investigation on animal study and work on aerosol formulation are required to develop KL4 peptide as siRNA carrier for clinical application. |
Description | Poster Presentation |
Persistent Identifier | http://hdl.handle.net/10722/245641 |
ISSN | 2023 Impact Factor: 2.0 2023 SCImago Journal Rankings: 0.571 |
DC Field | Value | Language |
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dc.contributor.author | Qiu, Y | - |
dc.contributor.author | Chow, MYT | - |
dc.contributor.author | Lam, JKW | - |
dc.date.accessioned | 2017-09-18T02:14:18Z | - |
dc.date.available | 2017-09-18T02:14:18Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | The 27th Drug Delivery to the Lungs (DDL27) Conference, Edinburgh, Scotland, UK, 7-9 December 2016. In Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2017, v. 30 n. 4, p. A-11, abstract no. 33 | - |
dc.identifier.issn | 1941-2711 | - |
dc.identifier.uri | http://hdl.handle.net/10722/245641 | - |
dc.description | Poster Presentation | - |
dc.description.abstract | Small interfering RNA (siRNA) has great potential for the treatment of various respiratory diseases through RNA interference (RNAi), but their clinical application is hindered by the lack of a safe and effective pulmonary delivery system. KL4 peptide is a synthetic amphipathic peptide that was previously developed to mimic the function of pulmonary surfactant protein B (SP-B). Its potential as siRNA carrier for pulmonary delivery was examined in this study. The cationic KL4 peptide was able to bind with siRNA to form complexes at 15:1 ratio (peptide to siRNA weight ratio) or above. It also mediated efficient gene silencing on lung epithelial cells, with 20:1 ratio as the optimal ratio for siRNA transfection. Furthermore, the KL4/siRNA complexes were not toxic at concentrations used for transfection in vitro. The study shows that KL4 peptide appears to be a promising candidate for siRNA delivery. Further investigation on animal study and work on aerosol formulation are required to develop KL4 peptide as siRNA carrier for clinical application. | - |
dc.language | eng | - |
dc.publisher | Mary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/products/product.aspx?pid=24 | - |
dc.relation.ispartof | Journal of Aerosol Medicine and Pulmonary Drug Delivery | - |
dc.rights | Journal of Aerosol Medicine and Pulmonary Drug Delivery. Copyright © Mary Ann Liebert, Inc. Publishers. | - |
dc.rights | Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/jamp.2017.ab02.abstracts | - |
dc.title | Synthetic KL4 peptide as new carrier of siRNA therapeutics for pulmonary delivery | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Lam, JKW: jkwlam@hku.hk | - |
dc.identifier.authority | Lam, JKW=rp01346 | - |
dc.identifier.hkuros | 277117 | - |
dc.identifier.volume | 30 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | A-11 | - |
dc.identifier.epage | A-11 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1941-2711 | - |