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- Publisher Website: 10.1016/j.ymeth.2010.12.022
- Scopus: eid_2-s2.0-79952707077
- PMID: 21185377
- WOS: WOS:000288878000006
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Article: Genetic screens using the piggyBac transposon
| Title | Genetic screens using the piggyBac transposon |
|---|---|
| Authors | |
| Keywords | Transposition Cancer Mouse Transposon Mutagenesis PiggyBac |
| Issue Date | 2011 |
| Citation | Methods, 2011, v. 53, n. 4, p. 366-371 How to Cite? |
| Abstract | Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. © 2010 Elsevier Inc. |
| Persistent Identifier | http://hdl.handle.net/10722/248982 |
| ISSN | 2021 Impact Factor: 4.647 2020 SCImago Journal Rankings: 2.080 |
| ISI Accession Number ID |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Chew, Su Kit | - |
| dc.contributor.author | Rad, Roland | - |
| dc.contributor.author | Futreal, P. Andrew | - |
| dc.contributor.author | Bradley, Allan | - |
| dc.contributor.author | Liu, Pentao | - |
| dc.date.accessioned | 2017-10-27T05:58:47Z | - |
| dc.date.available | 2017-10-27T05:58:47Z | - |
| dc.date.issued | 2011 | - |
| dc.identifier.citation | Methods, 2011, v. 53, n. 4, p. 366-371 | - |
| dc.identifier.issn | 1046-2023 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/248982 | - |
| dc.description.abstract | Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. © 2010 Elsevier Inc. | - |
| dc.language | eng | - |
| dc.relation.ispartof | Methods | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.subject | Transposition | - |
| dc.subject | Cancer | - |
| dc.subject | Mouse | - |
| dc.subject | Transposon | - |
| dc.subject | Mutagenesis | - |
| dc.subject | PiggyBac | - |
| dc.title | Genetic screens using the piggyBac transposon | - |
| dc.type | Article | - |
| dc.description.nature | published_or_final_version | - |
| dc.identifier.doi | 10.1016/j.ymeth.2010.12.022 | - |
| dc.identifier.pmid | 21185377 | - |
| dc.identifier.scopus | eid_2-s2.0-79952707077 | - |
| dc.identifier.volume | 53 | - |
| dc.identifier.issue | 4 | - |
| dc.identifier.spage | 366 | - |
| dc.identifier.epage | 371 | - |
| dc.identifier.eissn | 1095-9130 | - |
| dc.identifier.isi | WOS:000288878000006 | - |
| dc.identifier.issnl | 1046-2023 | - |