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Article: Reprogramming to pluripotency using designer TALE transcription factors targeting enhancers

TitleReprogramming to pluripotency using designer TALE transcription factors targeting enhancers
Authors
Issue Date2013
Citation
Stem Cell Reports, 2013, v. 1, n. 2, p. 183-197 How to Cite?
AbstractThe modular DNA recognition code of the transcription-activator-like effectors (TALEs) from plant pathogenic bacterial genus Xanthomonas provides a powerful genetic tool to create designer transcription factors (dTFs) targeting specific DNA sequences for manipulating gene expression. Previous studies have suggested critical roles of enhancers in gene regulation and reprogramming. Here, we report dTF activator targeting the distal enhancer of the Pou5f1 (Oct4) locus induces epigenetic changes, reactivates its expression, and substitutes exogenous OCT4 in reprogramming mouse embryonic fibroblast cells (MEFs) to induced pluripotent stem cells (iPSCs). Similarly, dTF activator targeting a Nanog enhancer activates Nanog expression and reprograms epiblast stem cells (EpiSCs) to iPSCs. Conversely, dTF repressors targeting the same genetic elements inhibit expression of these loci, and effectively block reprogramming. This study indicates that dTFs targeting specific enhancers can be used to study other biological processes such as transdifferentiation or directed differentiation of stem cells. © 2013 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/249078
ISSN
2023 Impact Factor: 5.9
2023 SCImago Journal Rankings: 2.518
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, Xuefei-
dc.contributor.authorYang, Jian-
dc.contributor.authorTsang, Jason C H-
dc.contributor.authorOoi, Jolene-
dc.contributor.authorWu, Donghai-
dc.contributor.authorLiu, Pentao-
dc.date.accessioned2017-10-27T05:59:03Z-
dc.date.available2017-10-27T05:59:03Z-
dc.date.issued2013-
dc.identifier.citationStem Cell Reports, 2013, v. 1, n. 2, p. 183-197-
dc.identifier.issn2213-6711-
dc.identifier.urihttp://hdl.handle.net/10722/249078-
dc.description.abstractThe modular DNA recognition code of the transcription-activator-like effectors (TALEs) from plant pathogenic bacterial genus Xanthomonas provides a powerful genetic tool to create designer transcription factors (dTFs) targeting specific DNA sequences for manipulating gene expression. Previous studies have suggested critical roles of enhancers in gene regulation and reprogramming. Here, we report dTF activator targeting the distal enhancer of the Pou5f1 (Oct4) locus induces epigenetic changes, reactivates its expression, and substitutes exogenous OCT4 in reprogramming mouse embryonic fibroblast cells (MEFs) to induced pluripotent stem cells (iPSCs). Similarly, dTF activator targeting a Nanog enhancer activates Nanog expression and reprograms epiblast stem cells (EpiSCs) to iPSCs. Conversely, dTF repressors targeting the same genetic elements inhibit expression of these loci, and effectively block reprogramming. This study indicates that dTFs targeting specific enhancers can be used to study other biological processes such as transdifferentiation or directed differentiation of stem cells. © 2013 The Authors.-
dc.languageeng-
dc.relation.ispartofStem Cell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleReprogramming to pluripotency using designer TALE transcription factors targeting enhancers-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.stemcr.2013.06.002-
dc.identifier.pmid24052952-
dc.identifier.scopuseid_2-s2.0-84881336110-
dc.identifier.volume1-
dc.identifier.issue2-
dc.identifier.spage183-
dc.identifier.epage197-
dc.identifier.isiWOS:000336632600007-
dc.identifier.issnl2213-6711-

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