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Article: Characterization of the single-cell derived bovine induced pluripotent stem cells

TitleCharacterization of the single-cell derived bovine induced pluripotent stem cells
Authors
KeywordsSingle-cell
Pluripotency
piggyBac
Bovine iPSCs
Issue Date2017
Citation
Tissue and Cell, 2017 How to Cite?
Abstract© 2017. Single-cell derived bovine induced pluripotent stem cells (iPSCs) were generated by the introduction of piggyBac transposons with CAG promoting transcription factors (Oct3/4, Sox2, Klf4 and cMyc). In the study, the bovine iPSCs colony from single cell could passage more than 50 passages after enzymatic dissociation into single cells. These bovine iPSCs cells kept the normal karyotype and displayed dome shaped clones similar to mouse embryonic stem cells. They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3/4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT-PCR. Additionally, single-cell derived bovine iPSCs formed embryoid bodies and teratomas that all subsequently gave rise to differentiated cells from all three embryonic germ layers. The results showed that our reprogramming method could obtain high efficiency single-cell cloning bovine iPSCs, and the efficiency of single cell cloning is 40%.
Persistent Identifierhttp://hdl.handle.net/10722/249165
ISSN
2023 Impact Factor: 2.7
2023 SCImago Journal Rankings: 0.588
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, Lixia-
dc.contributor.authorWang, Zixin-
dc.contributor.authorZhang, Jindun-
dc.contributor.authorYang, Jian-
dc.contributor.authorGao, Xuefei-
dc.contributor.authorWu, Baojiang-
dc.contributor.authorZhao, Gaoping-
dc.contributor.authorBao, Siqin-
dc.contributor.authorHu, Shuxiang-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorLi, Xihe-
dc.date.accessioned2017-10-27T05:59:16Z-
dc.date.available2017-10-27T05:59:16Z-
dc.date.issued2017-
dc.identifier.citationTissue and Cell, 2017-
dc.identifier.issn0040-8166-
dc.identifier.urihttp://hdl.handle.net/10722/249165-
dc.description.abstract© 2017. Single-cell derived bovine induced pluripotent stem cells (iPSCs) were generated by the introduction of piggyBac transposons with CAG promoting transcription factors (Oct3/4, Sox2, Klf4 and cMyc). In the study, the bovine iPSCs colony from single cell could passage more than 50 passages after enzymatic dissociation into single cells. These bovine iPSCs cells kept the normal karyotype and displayed dome shaped clones similar to mouse embryonic stem cells. They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3/4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT-PCR. Additionally, single-cell derived bovine iPSCs formed embryoid bodies and teratomas that all subsequently gave rise to differentiated cells from all three embryonic germ layers. The results showed that our reprogramming method could obtain high efficiency single-cell cloning bovine iPSCs, and the efficiency of single cell cloning is 40%.-
dc.languageeng-
dc.relation.ispartofTissue and Cell-
dc.subjectSingle-cell-
dc.subjectPluripotency-
dc.subjectpiggyBac-
dc.subjectBovine iPSCs-
dc.titleCharacterization of the single-cell derived bovine induced pluripotent stem cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.tice.2017.05.005-
dc.identifier.scopuseid_2-s2.0-85023608435-
dc.identifier.spagenull-
dc.identifier.epagenull-
dc.identifier.eissn1532-3072-
dc.identifier.isiWOS:000413740800001-
dc.identifier.issnl0040-8166-

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