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- Publisher Website: 10.1016/j.phymed.2017.10.006
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Article: Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo
Title | Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo |
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Authors | |
Keywords | Endothelial cells Ilex pubescens Ilexsaponin A1 Pro-angiogenesis Zebrafish |
Issue Date | 2017 |
Publisher | Elsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed |
Citation | Phytomedicine, 2017, v. 36, p. 229-237 How to Cite? |
Abstract | Background Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. Purpose To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. Study design Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively. Methods Pro-angiogenic effects of Ilexsaponin A1 were examined by assessing endothelial cell proliferation, migration, invasion and tube formation. The mechanism of pro-angiogenic effects was investigated by measuring the expression level of various signalling proteins. Furthermore, vascular endothelial growth factor (VEGF) tyrosine kinase inhibitor II (VRI)-induced vascular insufficient transgenic zebrafish model was used to confirm the results of the HUVECs results in vivo. Results Ilexsaponin A1 significantly promoted cell proliferation, migration, invasion and tube formation in HUVECs, and rescued blood vessel loss in VRI-induced vascular insufficient zebrafish. Ilexsaponin A1 upregulated p-Akt, p-mTOR, p-Src, p-FAK, p-MEK, and p-Erk1/2 in HUVECs. Conclusion This study showed that Ilexsaponin A1 exhibits pro-angiogenic activity in HUVECs and VRI-induced vascular insufficient zebrafish, probably by activating Akt/mTOR, MAPK/ERK and Src- and FAK-dependent signalling pathways. The findings suggest that Ilexsaponin A1 and probably I. pubescens, a major source of Ilexsaponin A1, could be developed as a potential therapeutic agent for preventing or treating cardiovascular diseases and/or other diseases related to vascular insufficiency. © 2017 Elsevier GmbH |
Persistent Identifier | http://hdl.handle.net/10722/250021 |
ISSN | 2023 Impact Factor: 6.7 2023 SCImago Journal Rankings: 1.267 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, J | - |
dc.contributor.author | Zhang, J | - |
dc.contributor.author | Zou, L | - |
dc.contributor.author | Lee, SMY | - |
dc.contributor.author | Yang, C | - |
dc.contributor.author | Seto, SW | - |
dc.contributor.author | Leung, GPH | - |
dc.date.accessioned | 2017-12-20T09:19:31Z | - |
dc.date.available | 2017-12-20T09:19:31Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Phytomedicine, 2017, v. 36, p. 229-237 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.uri | http://hdl.handle.net/10722/250021 | - |
dc.description.abstract | Background Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. Purpose To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. Study design Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively. Methods Pro-angiogenic effects of Ilexsaponin A1 were examined by assessing endothelial cell proliferation, migration, invasion and tube formation. The mechanism of pro-angiogenic effects was investigated by measuring the expression level of various signalling proteins. Furthermore, vascular endothelial growth factor (VEGF) tyrosine kinase inhibitor II (VRI)-induced vascular insufficient transgenic zebrafish model was used to confirm the results of the HUVECs results in vivo. Results Ilexsaponin A1 significantly promoted cell proliferation, migration, invasion and tube formation in HUVECs, and rescued blood vessel loss in VRI-induced vascular insufficient zebrafish. Ilexsaponin A1 upregulated p-Akt, p-mTOR, p-Src, p-FAK, p-MEK, and p-Erk1/2 in HUVECs. Conclusion This study showed that Ilexsaponin A1 exhibits pro-angiogenic activity in HUVECs and VRI-induced vascular insufficient zebrafish, probably by activating Akt/mTOR, MAPK/ERK and Src- and FAK-dependent signalling pathways. The findings suggest that Ilexsaponin A1 and probably I. pubescens, a major source of Ilexsaponin A1, could be developed as a potential therapeutic agent for preventing or treating cardiovascular diseases and/or other diseases related to vascular insufficiency. © 2017 Elsevier GmbH | - |
dc.language | eng | - |
dc.publisher | Elsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed | - |
dc.relation.ispartof | Phytomedicine | - |
dc.rights | Posting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Endothelial cells | - |
dc.subject | Ilex pubescens | - |
dc.subject | Ilexsaponin A1 | - |
dc.subject | Pro-angiogenesis | - |
dc.subject | Zebrafish | - |
dc.title | Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo | - |
dc.type | Article | - |
dc.identifier.email | Leung, GPH: gphleung@hkucc.hku.hk | - |
dc.identifier.authority | Leung, GPH=rp00234 | - |
dc.identifier.doi | 10.1016/j.phymed.2017.10.006 | - |
dc.identifier.scopus | eid_2-s2.0-85032346640 | - |
dc.identifier.hkuros | 283875 | - |
dc.identifier.volume | 36 | - |
dc.identifier.spage | 229 | - |
dc.identifier.epage | 237 | - |
dc.identifier.isi | WOS:000415693500028 | - |
dc.publisher.place | Germany | - |
dc.identifier.issnl | 0944-7113 | - |