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- Publisher Website: 10.1093/mmy/myx118
- Scopus: eid_2-s2.0-85054956697
- PMID: 29228397
- WOS: WOS:000448007600007
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Article: Yeast identification by sequencing, biochemical kits, MALDI–TOF MS and rep-PCR DNA fingerprinting
Title | Yeast identification by sequencing, biochemical kits, MALDI–TOF MS and rep-PCR DNA fingerprinting |
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Authors | |
Keywords | DNA sequencing Identification MALDI-TOF MS rep-PCR DNA fingerprinting Yeasts |
Issue Date | 2017 |
Publisher | Informa Healthcare. The Journal's web site is located at https://academic.oup.com/mmy/ |
Citation | Medical Mycology, 2017, v. 56 n. 7, p. 816-827 How to Cite? |
Abstract | No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting. 28S nrDNA and ITS sequencing showed complete agreement on the identities of the 71 isolates. Using sequencing results as the standard, 78.9% and 71.8% isolates were correctly identified using the API 20C AUX and Vitek 2 YST ID Card systems, respectively; and 90.1% and 80.3% isolates were correctly identified using the Bruker and Vitek MALDI-TOF MS platforms, respectively. Of the 18 strains belonging to the Candida parapsilosis species complex tested by DiversiLab automated rep-PCR DNA fingerprinting, all were identified only as Candida parapsilosis with similarities ≥93.2%, indicating the misidentification of Candida metapsilosis and Candida orthopsilosis. However, hierarchical cluster analysis of the rep-PCR DNA fingerprints of these three species within this species complex formed three different discrete clusters, indicating that this technology can potentially differentiate the three species. To achieve higher accuracies of identification, the databases of commercial biochemical test kits, MALDI-TOF MS platforms, and DiversiLab automated rep-PCR DNA fingerprinting needs further enrichment, particularly for uncommonly encountered yeast species. |
Persistent Identifier | http://hdl.handle.net/10722/250546 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.582 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhao, Y | - |
dc.contributor.author | Tsang, CC | - |
dc.contributor.author | Xiao, M | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Lau, SKP | - |
dc.contributor.author | Kong, F | - |
dc.contributor.author | Xu, Y | - |
dc.contributor.author | Woo, PCY | - |
dc.date.accessioned | 2018-01-18T04:28:47Z | - |
dc.date.available | 2018-01-18T04:28:47Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Medical Mycology, 2017, v. 56 n. 7, p. 816-827 | - |
dc.identifier.issn | 1369-3786 | - |
dc.identifier.uri | http://hdl.handle.net/10722/250546 | - |
dc.description.abstract | No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting. 28S nrDNA and ITS sequencing showed complete agreement on the identities of the 71 isolates. Using sequencing results as the standard, 78.9% and 71.8% isolates were correctly identified using the API 20C AUX and Vitek 2 YST ID Card systems, respectively; and 90.1% and 80.3% isolates were correctly identified using the Bruker and Vitek MALDI-TOF MS platforms, respectively. Of the 18 strains belonging to the Candida parapsilosis species complex tested by DiversiLab automated rep-PCR DNA fingerprinting, all were identified only as Candida parapsilosis with similarities ≥93.2%, indicating the misidentification of Candida metapsilosis and Candida orthopsilosis. However, hierarchical cluster analysis of the rep-PCR DNA fingerprints of these three species within this species complex formed three different discrete clusters, indicating that this technology can potentially differentiate the three species. To achieve higher accuracies of identification, the databases of commercial biochemical test kits, MALDI-TOF MS platforms, and DiversiLab automated rep-PCR DNA fingerprinting needs further enrichment, particularly for uncommonly encountered yeast species. | - |
dc.language | eng | - |
dc.publisher | Informa Healthcare. The Journal's web site is located at https://academic.oup.com/mmy/ | - |
dc.relation.ispartof | Medical Mycology | - |
dc.rights | Medical Mycology. Copyright © Informa Healthcare. | - |
dc.subject | DNA sequencing | - |
dc.subject | Identification | - |
dc.subject | MALDI-TOF MS | - |
dc.subject | rep-PCR DNA fingerprinting | - |
dc.subject | Yeasts | - |
dc.title | Yeast identification by sequencing, biochemical kits, MALDI–TOF MS and rep-PCR DNA fingerprinting | - |
dc.type | Article | - |
dc.identifier.email | Tsang, CC: microbioct@connect.hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Lau, SKP: skplau@hkucc.hku.hk | - |
dc.identifier.email | Woo, PCY: pcywoo@hkucc.hku.hk | - |
dc.identifier.authority | Tsang, CC=rp02492 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Lau, SKP=rp00486 | - |
dc.identifier.authority | Woo, PCY=rp00430 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1093/mmy/myx118 | - |
dc.identifier.pmid | 29228397 | - |
dc.identifier.scopus | eid_2-s2.0-85054956697 | - |
dc.identifier.hkuros | 283893 | - |
dc.identifier.hkuros | 304170 | - |
dc.identifier.volume | 56 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 816 | - |
dc.identifier.epage | 827 | - |
dc.identifier.isi | WOS:000448007600007 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1369-3786 | - |