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postgraduate thesis: ARID1A in cervical cancer
Title | ARID1A in cervical cancer |
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Authors | |
Issue Date | 2017 |
Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
Citation | Hira, K.. (2017). ARID1A in cervical cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. |
Abstract | Background: Persistent HPV infection has been established as a necessary factor for cervical carcinogenesis. However, evidence supporting the existence of HPV-negative cervical cancer is emerging. A recent comprehensive genomic-wide characterization of cervical cancers indicated that HPV-negative cervical cancers are likely to harbor loss-of-function mutations in the gene encoding a SWI/SNF complex component AT-rich interactive domain-containing protein 1A (ARID1A).
Objective: The aim of this study was to determine the mutation status and protein expression of ARID1A in HPV-negative cervical cancer.
Methods: Exons 2-20 of the ARID1A gene from six human cervical cancer cell lines, HeLa, SiHa, C4-1, CaSki, ME-180 and C33A, were amplified by PCR and sequenced with Sanger sequencing. ARID1A protein expression in 9 HPV-positive and 10 HPV-negative histologically confirmed cervical cancer, and two human cervical cancer cell lines, HeLa and C33A, was evaluated with immunohistochemistry.
Results: A frameshift mutation was found in exon 7 (c.2274delG) in C33A, and a substitution in exon 13 (c.3471G>A) in C4-1. On the other hand, ARID1A protein expression could be detected in 8 (88.9%) of HPV-positive and 9 (90%) of HPV-negative cervical cancer. Thus, ARID1A protein positivity was not statistically different between HPV-positive and HPV-negative cervical cancer (p=0.468). However, there was difference in ARID1A protein expression between HPV-positive cervical cancer cell line HeLa and HPV-negative cervical cancer cell line C33A. ARID1A protein expression was weakly positive in the HeLa cell line but negative in the C33A cell line.
Conclusions: Results in this study supported that ARID1A mutations are present in HPV-negative cervical cancer, but loss of ARID1A protein was not evident in our clinical tissue samples.
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Degree | Master of Medical Sciences |
Subject | Cervix uteri - Cancer - Genetic aspects |
Dept/Program | Pathology |
Persistent Identifier | http://hdl.handle.net/10722/251342 |
DC Field | Value | Language |
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dc.contributor.author | Hira, Khan | - |
dc.date.accessioned | 2018-02-27T09:53:42Z | - |
dc.date.available | 2018-02-27T09:53:42Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Hira, K.. (2017). ARID1A in cervical cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. | - |
dc.identifier.uri | http://hdl.handle.net/10722/251342 | - |
dc.description.abstract | Background: Persistent HPV infection has been established as a necessary factor for cervical carcinogenesis. However, evidence supporting the existence of HPV-negative cervical cancer is emerging. A recent comprehensive genomic-wide characterization of cervical cancers indicated that HPV-negative cervical cancers are likely to harbor loss-of-function mutations in the gene encoding a SWI/SNF complex component AT-rich interactive domain-containing protein 1A (ARID1A). Objective: The aim of this study was to determine the mutation status and protein expression of ARID1A in HPV-negative cervical cancer. Methods: Exons 2-20 of the ARID1A gene from six human cervical cancer cell lines, HeLa, SiHa, C4-1, CaSki, ME-180 and C33A, were amplified by PCR and sequenced with Sanger sequencing. ARID1A protein expression in 9 HPV-positive and 10 HPV-negative histologically confirmed cervical cancer, and two human cervical cancer cell lines, HeLa and C33A, was evaluated with immunohistochemistry. Results: A frameshift mutation was found in exon 7 (c.2274delG) in C33A, and a substitution in exon 13 (c.3471G>A) in C4-1. On the other hand, ARID1A protein expression could be detected in 8 (88.9%) of HPV-positive and 9 (90%) of HPV-negative cervical cancer. Thus, ARID1A protein positivity was not statistically different between HPV-positive and HPV-negative cervical cancer (p=0.468). However, there was difference in ARID1A protein expression between HPV-positive cervical cancer cell line HeLa and HPV-negative cervical cancer cell line C33A. ARID1A protein expression was weakly positive in the HeLa cell line but negative in the C33A cell line. Conclusions: Results in this study supported that ARID1A mutations are present in HPV-negative cervical cancer, but loss of ARID1A protein was not evident in our clinical tissue samples. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.lcsh | Cervix uteri - Cancer - Genetic aspects | - |
dc.title | ARID1A in cervical cancer | - |
dc.type | PG_Thesis | - |
dc.description.thesisname | Master of Medical Sciences | - |
dc.description.thesislevel | Master | - |
dc.description.thesisdiscipline | Pathology | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.5353/th_991043982793803414 | - |
dc.date.hkucongregation | 2017 | - |
dc.identifier.mmsid | 991043982793803414 | - |