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Conference Paper: Synthesis and Selection of DNA-encoded Chemical Libraries
Title | Synthesis and Selection of DNA-encoded Chemical Libraries |
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Authors | |
Issue Date | 2016 |
Citation | The Fourth Asian Chemical Biology Conference (ACBC 2016), Kaohsiung, Taiwan, 28 November - 1 December 2016
How to Cite? |
Abstract | Originally in 1992, Brenner and Lerner proposed the concept of using DNA as tags to encode chemical reactions in combinatorial library synthesis. During the past two decades, a variety of strategies of DNA-encoded libraries (DEL) have been developed. Benefiting from the high encoding capacity of DNA molecules, today’s DELs can be pre-pared with an extremely large number of compounds. Selections of DELs against biological targets can be accomplished using highly sensitive PCR amplification and ultra-high-throughput DNA sequencing technology. Selections of DELs have already generated many novel ligands against biological targets and have become a new discovery modality for researchers in both academia and pharmaceutical industry. Here we report our recent progresses on the strategies for the synthesis and selection of DNA-encoded libraries. Notably, we developed a “universal template” method able to prepare the entire library with a single DNA template; our novel selection method also enables the selection of DELs against previously intractable drug targets. These methods are being implemented in a variety of drug discovery programs. |
Persistent Identifier | http://hdl.handle.net/10722/253831 |
DC Field | Value | Language |
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dc.contributor.author | Zhou, Y | - |
dc.contributor.author | Shi, B | - |
dc.contributor.author | Li, X | - |
dc.date.accessioned | 2018-05-30T02:46:18Z | - |
dc.date.available | 2018-05-30T02:46:18Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The Fourth Asian Chemical Biology Conference (ACBC 2016), Kaohsiung, Taiwan, 28 November - 1 December 2016 | - |
dc.identifier.uri | http://hdl.handle.net/10722/253831 | - |
dc.description.abstract | Originally in 1992, Brenner and Lerner proposed the concept of using DNA as tags to encode chemical reactions in combinatorial library synthesis. During the past two decades, a variety of strategies of DNA-encoded libraries (DEL) have been developed. Benefiting from the high encoding capacity of DNA molecules, today’s DELs can be pre-pared with an extremely large number of compounds. Selections of DELs against biological targets can be accomplished using highly sensitive PCR amplification and ultra-high-throughput DNA sequencing technology. Selections of DELs have already generated many novel ligands against biological targets and have become a new discovery modality for researchers in both academia and pharmaceutical industry. Here we report our recent progresses on the strategies for the synthesis and selection of DNA-encoded libraries. Notably, we developed a “universal template” method able to prepare the entire library with a single DNA template; our novel selection method also enables the selection of DELs against previously intractable drug targets. These methods are being implemented in a variety of drug discovery programs. | - |
dc.language | eng | - |
dc.relation.ispartof | The Asian Chemical Biology Conference | - |
dc.title | Synthesis and Selection of DNA-encoded Chemical Libraries | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhou, Y: yuchow@hku.hk | - |
dc.identifier.email | Shi, B: shibbing@hku.hk | - |
dc.identifier.email | Li, X: xiaoyuli@hku.hk | - |
dc.identifier.authority | Li, X=rp02080 | - |
dc.identifier.hkuros | 274709 | - |
dc.publisher.place | Kaohsiung, Taiwan | - |