File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1364/OE.23.001879
- Scopus: eid_2-s2.0-84921751509
- WOS: WOS:000349166100123
Supplementary
- Citations:
- Appears in Collections:
Article: A user-friendly two-color super-resolution localization microscope
Title | A user-friendly two-color super-resolution localization microscope |
---|---|
Authors | |
Issue Date | 2015 |
Citation | Optics Express, 2015, v. 23, n. 2, p. 1879-1887 How to Cite? |
Abstract | © 2015 Optical Society of America. We report a robust two-color method for super-resolution localization microscopy. Two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera. Active sample locking stabilizes sample with 1nm accuracy during imaging. With over 4,000 photons emitted from both dyes, two-color super-resolution images with high-quality were obtained in a wide range of samples including cell cultures, tissue sections and yeast cells. |
Persistent Identifier | http://hdl.handle.net/10722/255951 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhao, Teng | - |
dc.contributor.author | Wang, Ying | - |
dc.contributor.author | Zhai, Yuanliang | - |
dc.contributor.author | Qu, Xiaoxuan | - |
dc.contributor.author | Cheng, Aifang | - |
dc.contributor.author | Du, Shengwang | - |
dc.contributor.author | Loy, M. M.T. | - |
dc.date.accessioned | 2018-07-16T06:14:09Z | - |
dc.date.available | 2018-07-16T06:14:09Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Optics Express, 2015, v. 23, n. 2, p. 1879-1887 | - |
dc.identifier.uri | http://hdl.handle.net/10722/255951 | - |
dc.description.abstract | © 2015 Optical Society of America. We report a robust two-color method for super-resolution localization microscopy. Two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera. Active sample locking stabilizes sample with 1nm accuracy during imaging. With over 4,000 photons emitted from both dyes, two-color super-resolution images with high-quality were obtained in a wide range of samples including cell cultures, tissue sections and yeast cells. | - |
dc.language | eng | - |
dc.relation.ispartof | Optics Express | - |
dc.title | A user-friendly two-color super-resolution localization microscope | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1364/OE.23.001879 | - |
dc.identifier.scopus | eid_2-s2.0-84921751509 | - |
dc.identifier.volume | 23 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 1879 | - |
dc.identifier.epage | 1887 | - |
dc.identifier.eissn | 1094-4087 | - |
dc.identifier.isi | WOS:000349166100123 | - |
dc.identifier.issnl | 1094-4087 | - |