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Article: TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells
Title | TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells |
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Authors | |
Issue Date | 2017 |
Publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/mi/index.html |
Citation | Mediators of Inflammation, 2017, v. 2017, p. 7462945:1-7462945:12 How to Cite? |
Abstract | Proinflammatory cytokines such as TNF-α and type I interferons (IFN) are pathogenic signatures of systemic lupus erythematosus, and plasmacytoid dendritic cells (pDCs) play a major role by predominantly producing IFN-α. Given the rise of importance in identifying tumor necrosis stimulated gene 6 (TSG-6) as a key anti-inflammatory regulator, we investigate its function and its ability to counteract proinflammatory cytokine secretion by pDCs in vitro. CpG-A and R837 induced significant endogenous TSG-6 expression in the pDC cell-line GEN2.2. Following recombinant human TSG-6 treatment and CpG-A or R837 stimulation, significant reduction in IFN-α and TNF-α was observed in healthy donors’ pDCs, and the same phenomenon was confirmed in GEN2.2. By CD44 blocking assay, we deduced that the suppressive effect of TSG-6 is mediated by CD44, by reducing IRF-7 phosphorylation. Our findings suggest that TSG-6 and its downstream signalling pathway could potentially be targeted to modulate proinflammatory cytokine expression in pDCs. |
Persistent Identifier | http://hdl.handle.net/10722/256358 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.043 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | KUI, L | - |
dc.contributor.author | Chan, GCF | - |
dc.contributor.author | Lee, PPW | - |
dc.date.accessioned | 2018-07-20T06:33:24Z | - |
dc.date.available | 2018-07-20T06:33:24Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Mediators of Inflammation, 2017, v. 2017, p. 7462945:1-7462945:12 | - |
dc.identifier.issn | 0962-9351 | - |
dc.identifier.uri | http://hdl.handle.net/10722/256358 | - |
dc.description.abstract | Proinflammatory cytokines such as TNF-α and type I interferons (IFN) are pathogenic signatures of systemic lupus erythematosus, and plasmacytoid dendritic cells (pDCs) play a major role by predominantly producing IFN-α. Given the rise of importance in identifying tumor necrosis stimulated gene 6 (TSG-6) as a key anti-inflammatory regulator, we investigate its function and its ability to counteract proinflammatory cytokine secretion by pDCs in vitro. CpG-A and R837 induced significant endogenous TSG-6 expression in the pDC cell-line GEN2.2. Following recombinant human TSG-6 treatment and CpG-A or R837 stimulation, significant reduction in IFN-α and TNF-α was observed in healthy donors’ pDCs, and the same phenomenon was confirmed in GEN2.2. By CD44 blocking assay, we deduced that the suppressive effect of TSG-6 is mediated by CD44, by reducing IRF-7 phosphorylation. Our findings suggest that TSG-6 and its downstream signalling pathway could potentially be targeted to modulate proinflammatory cytokine expression in pDCs. | - |
dc.language | eng | - |
dc.publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/mi/index.html | - |
dc.relation.ispartof | Mediators of Inflammation | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells | - |
dc.type | Article | - |
dc.identifier.email | Chan, GCF: gcfchan@hku.hk | - |
dc.identifier.email | Lee, PPW: ppwlee@hku.hk | - |
dc.identifier.authority | Chan, GCF=rp00431 | - |
dc.identifier.authority | Lee, PPW=rp00462 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1155/2017/7462945 | - |
dc.identifier.scopus | eid_2-s2.0-85016045887 | - |
dc.identifier.hkuros | 286122 | - |
dc.identifier.volume | 2017 | - |
dc.identifier.spage | 7462945:1 | - |
dc.identifier.epage | 7462945:12 | - |
dc.identifier.isi | WOS:000397878900001 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0962-9351 | - |