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Conference Paper: Chicken epiregulin (ER) gene: cDNA cloning, genomic organization, and regulation of its mRNA expression in ovarian granulosa cells
Title | Chicken epiregulin (ER) gene: cDNA cloning, genomic organization, and regulation of its mRNA expression in ovarian granulosa cells |
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Authors | |
Keywords | Epiregulin Chicken Ovary |
Issue Date | 2007 |
Publisher | ADSA-PSA-AMPA-ASAS. The Abstracts' web site is located at https://www.jtmtg.org/JAM/2007/abstracts/2007-JAM-Abstracts.pdf |
Citation | The Joint Annual Meeting of the ADSA, PSA, AMPA, ASAS (American Dairy Science Association, Poultry Science Association, Asociación Mexicana de Producción Animal, and the American Society of Animal Science), San Antonio, Texas, USA, July 8-12, 2007. In Joint Annual Meeting Abstracts, 2007, p. 651, abstract no. 892 How to Cite? |
Abstract | Growing evidence suggests that epiregulin (ER), a ligand of epidermal growth factor receptor (EGFR), is involved in controlling follicular development in mammals. However, there is no information on its
expression in the ovary of non-mammalian species including chicken. In this study, we first cloned the full-length cDNA of chicken epiregulin gene from ovary. The cloned cDNA is 948 bp in length and encodes a membrane-anchored precursor of 153 amino aicds, which shares high sequence identity (~59%) with its mammalian counterpart. Genomic structural analysis shows that chicken epiregulin gene spans approximately 8 kb on chromosome 4 and consists of 5 exons. Using RT-PCR assay, ER mRNA was detected to be expressed in embryonic (embryonic day 20), sexually immature (3 week), and mature ovaries (6 chickens used at each stage), suggesting a role of ER in ovarian development. Compared with the other six EGFR ligands, epiregulin appeared to be expressed at a low level. However, using semiquantitative RT-PCR assay, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) have been demonstrated to be
capable of inducing ER mRNA expression significantly in cultured ovarian granulosa cells from preovulatory follicle (F1) in a timedependent manner. The expression of epiregulin reached the maximum
at 4 h and was sustained at 24 h post-treatment. Moreover, phorbol 12-myristate 13-acetate (PMA), a potent protein kinase C (PKC) activator, could also significantly induce epiregulin mRNA expression
in a dose-dependent manner, suggesting that PKC (or PKC-activated) signaling pathway may be involved in controlling ER expression in the chicken ovary. These findings suggest that ER is a potential paracrine/autocrine factor involved in controlling ovarian development and its expression is regulated by local ovarian factors including EGFR ligands from chicken ovary. |
Description | Physiology & Endocrinology - Livestock and Poultry: Reproductive Physiology - no. 892 2007 Joint Meeting Abstracts is a combined issue of Journal of Animal Science, V. 85, Suppl. 1 / Journal of Dairy Science, V. 90, Suppl. 1 / Poultry Science, V. 86, Suppl, 1 |
Persistent Identifier | http://hdl.handle.net/10722/257958 |
DC Field | Value | Language |
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dc.contributor.author | Wang, Y | - |
dc.contributor.author | Li, J | - |
dc.contributor.author | Leung, FCC | - |
dc.date.accessioned | 2018-08-21T01:59:04Z | - |
dc.date.available | 2018-08-21T01:59:04Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | The Joint Annual Meeting of the ADSA, PSA, AMPA, ASAS (American Dairy Science Association, Poultry Science Association, Asociación Mexicana de Producción Animal, and the American Society of Animal Science), San Antonio, Texas, USA, July 8-12, 2007. In Joint Annual Meeting Abstracts, 2007, p. 651, abstract no. 892 | - |
dc.identifier.uri | http://hdl.handle.net/10722/257958 | - |
dc.description | Physiology & Endocrinology - Livestock and Poultry: Reproductive Physiology - no. 892 | - |
dc.description | 2007 Joint Meeting Abstracts is a combined issue of Journal of Animal Science, V. 85, Suppl. 1 / Journal of Dairy Science, V. 90, Suppl. 1 / Poultry Science, V. 86, Suppl, 1 | - |
dc.description.abstract | Growing evidence suggests that epiregulin (ER), a ligand of epidermal growth factor receptor (EGFR), is involved in controlling follicular development in mammals. However, there is no information on its expression in the ovary of non-mammalian species including chicken. In this study, we first cloned the full-length cDNA of chicken epiregulin gene from ovary. The cloned cDNA is 948 bp in length and encodes a membrane-anchored precursor of 153 amino aicds, which shares high sequence identity (~59%) with its mammalian counterpart. Genomic structural analysis shows that chicken epiregulin gene spans approximately 8 kb on chromosome 4 and consists of 5 exons. Using RT-PCR assay, ER mRNA was detected to be expressed in embryonic (embryonic day 20), sexually immature (3 week), and mature ovaries (6 chickens used at each stage), suggesting a role of ER in ovarian development. Compared with the other six EGFR ligands, epiregulin appeared to be expressed at a low level. However, using semiquantitative RT-PCR assay, epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) have been demonstrated to be capable of inducing ER mRNA expression significantly in cultured ovarian granulosa cells from preovulatory follicle (F1) in a timedependent manner. The expression of epiregulin reached the maximum at 4 h and was sustained at 24 h post-treatment. Moreover, phorbol 12-myristate 13-acetate (PMA), a potent protein kinase C (PKC) activator, could also significantly induce epiregulin mRNA expression in a dose-dependent manner, suggesting that PKC (or PKC-activated) signaling pathway may be involved in controlling ER expression in the chicken ovary. These findings suggest that ER is a potential paracrine/autocrine factor involved in controlling ovarian development and its expression is regulated by local ovarian factors including EGFR ligands from chicken ovary. | - |
dc.language | eng | - |
dc.publisher | ADSA-PSA-AMPA-ASAS. The Abstracts' web site is located at https://www.jtmtg.org/JAM/2007/abstracts/2007-JAM-Abstracts.pdf | - |
dc.relation.ispartof | ADSA-PSA-AMPA-ASAS Joint Annual Meeting | - |
dc.subject | Epiregulin | - |
dc.subject | Chicken | - |
dc.subject | Ovary | - |
dc.title | Chicken epiregulin (ER) gene: cDNA cloning, genomic organization, and regulation of its mRNA expression in ovarian granulosa cells | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wang, Y: cdwyj@yahoo.com | - |
dc.identifier.email | Leung, FCC: fcleung@hkucc.hku.hk | - |
dc.identifier.authority | Wang, Y=rp00801 | - |
dc.identifier.authority | Leung, FCC=rp00731 | - |
dc.identifier.hkuros | 167659 | - |
dc.identifier.spage | 651 | - |
dc.identifier.epage | 651 | - |
dc.publisher.place | United States | - |