File Download

There are no files associated with this item.

Supplementary

Conference Paper: Effective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis

TitleEffective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis
Authors
Issue Date2017
PublisherThe Aerosol Society.
Citation
Drug Delivery to the Lungs Conference (DDL), Edinburgh, UK, 6-8 December 2017 How to Cite?
AbstractSmall interfering RNA (siRNA) have been widely investigated for the treatment of various respiratory diseases. A vector with good safety and high efficacy for pulmonary siRNA delivery is a major problem that needs to be addressed. The synthetic peptide KL4 is a 21-residue peptide containing hydrophobic leucine interspersed with cationic lysine, which was designed to mimic the function of surfactant protein B (SP-B). Previous result showed that the KL4/siRNA complexes can successfully induce gene silencing in lung epithelial cells. The aim of this study was to further study the uptake profile of the KL4/siRNA complexes, in order to optimize the system for pulmonary delivery. The result showed that clathrin-mediated endocytosis was involved in cellular uptake of KL4/siRNA complexes. Future study will focus on the in vivo transfection through intratracheal delivery of the complexes.
DescriptionPoster presentation
Persistent Identifierhttp://hdl.handle.net/10722/258163

 

DC FieldValueLanguage
dc.contributor.authorQiu, Y-
dc.contributor.authorChow, YT-
dc.contributor.authorLam, JKW-
dc.date.accessioned2018-08-22T01:33:59Z-
dc.date.available2018-08-22T01:33:59Z-
dc.date.issued2017-
dc.identifier.citationDrug Delivery to the Lungs Conference (DDL), Edinburgh, UK, 6-8 December 2017-
dc.identifier.urihttp://hdl.handle.net/10722/258163-
dc.descriptionPoster presentation-
dc.description.abstractSmall interfering RNA (siRNA) have been widely investigated for the treatment of various respiratory diseases. A vector with good safety and high efficacy for pulmonary siRNA delivery is a major problem that needs to be addressed. The synthetic peptide KL4 is a 21-residue peptide containing hydrophobic leucine interspersed with cationic lysine, which was designed to mimic the function of surfactant protein B (SP-B). Previous result showed that the KL4/siRNA complexes can successfully induce gene silencing in lung epithelial cells. The aim of this study was to further study the uptake profile of the KL4/siRNA complexes, in order to optimize the system for pulmonary delivery. The result showed that clathrin-mediated endocytosis was involved in cellular uptake of KL4/siRNA complexes. Future study will focus on the in vivo transfection through intratracheal delivery of the complexes.-
dc.languageeng-
dc.publisherThe Aerosol Society.-
dc.relation.ispartofDrug Delivery to the Lungs Conference (DDL) 2017-
dc.titleEffective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis-
dc.typeConference_Paper-
dc.identifier.emailChow, YT: mytchow@hku.hk-
dc.identifier.emailLam, JKW: jkwlam@hku.hk-
dc.identifier.authorityLam, JKW=rp01346-
dc.identifier.hkuros286666-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats