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Article: Comparative mRNA-seq analysis reveals the improved EPS production machinery in Streptococcus thermophilus ASCC 1275 during optimized milk fermentation
Title | Comparative mRNA-seq analysis reveals the improved EPS production machinery in Streptococcus thermophilus ASCC 1275 during optimized milk fermentation |
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Authors | |
Keywords | Exopolysaccharide Milk fermentation PH Streptococcus thermophilus Transcriptome |
Issue Date | 2018 |
Publisher | Frontiers Research Foundation. |
Citation | Frontiers in Microbiology, Food Microbiology Section, 2018, v. 9 n. MAR, article no. 445 How to Cite? |
Abstract | Exo-polysaccharide (EPS) produced by dairy starters plays critical roles in improving texture and functionalities of fermented dairy products. One of such high EPS producers, Streptococcus thermophilus ASCC 1275 (ST1275) was used as a model dairy strain to understand the stimulation of its EPS production under optimal milk fermentation conditions. The mRNA-seq analysis and targeted pathway analysis indicate that genes associated with lactose (milk sugar) catabolism, EPS assembly, proteolytic activity, and arginine/methionine/cysteine synthesis and transport in ST1275 were significantly up-regulated under the optimized conditions of pH 5.5, 40°C, or WPI supplementation compared to that of pH 6.5 and 37°C, respectively. This indicates that genes involved in above metabolisms cooperate together for improving EPS yield from ST1275. This study provides a global view map on potential targeted pathways and specific genes accounted for enhanced EPS production in Str. thermophilus and that could be modulated by fermentation conditions. |
Persistent Identifier | http://hdl.handle.net/10722/260857 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.065 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wu, Q | - |
dc.contributor.author | Shah, N | - |
dc.date.accessioned | 2018-09-14T08:48:35Z | - |
dc.date.available | 2018-09-14T08:48:35Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Frontiers in Microbiology, Food Microbiology Section, 2018, v. 9 n. MAR, article no. 445 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/10722/260857 | - |
dc.description.abstract | Exo-polysaccharide (EPS) produced by dairy starters plays critical roles in improving texture and functionalities of fermented dairy products. One of such high EPS producers, Streptococcus thermophilus ASCC 1275 (ST1275) was used as a model dairy strain to understand the stimulation of its EPS production under optimal milk fermentation conditions. The mRNA-seq analysis and targeted pathway analysis indicate that genes associated with lactose (milk sugar) catabolism, EPS assembly, proteolytic activity, and arginine/methionine/cysteine synthesis and transport in ST1275 were significantly up-regulated under the optimized conditions of pH 5.5, 40°C, or WPI supplementation compared to that of pH 6.5 and 37°C, respectively. This indicates that genes involved in above metabolisms cooperate together for improving EPS yield from ST1275. This study provides a global view map on potential targeted pathways and specific genes accounted for enhanced EPS production in Str. thermophilus and that could be modulated by fermentation conditions. | - |
dc.language | eng | - |
dc.publisher | Frontiers Research Foundation. | - |
dc.relation.ispartof | Frontiers in Microbiology, Food Microbiology Section | - |
dc.rights | This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Exopolysaccharide | - |
dc.subject | Milk fermentation | - |
dc.subject | PH | - |
dc.subject | Streptococcus thermophilus | - |
dc.subject | Transcriptome | - |
dc.title | Comparative mRNA-seq analysis reveals the improved EPS production machinery in Streptococcus thermophilus ASCC 1275 during optimized milk fermentation | - |
dc.type | Article | - |
dc.identifier.email | Shah, N: npshah@hku.hk | - |
dc.identifier.authority | Shah, N=rp01571 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3389/fmicb.2018.00445 | - |
dc.identifier.scopus | eid_2-s2.0-85043529963 | - |
dc.identifier.hkuros | 290726 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | MAR | - |
dc.identifier.spage | article no. 445 | - |
dc.identifier.epage | article no. 445 | - |
dc.identifier.isi | WOS:000427266400002 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1664-302X | - |