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Article: Simple and Rapid Tissue Clearing Method for Three-Dimensional Histology of the Pancreas
Title | Simple and Rapid Tissue Clearing Method for Three-Dimensional Histology of the Pancreas |
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Authors | |
Keywords | CLARITY pancreas simple three-dimensional imaging tissue clearing |
Issue Date | 2017 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://cda.currentprotocols.com/WileyCDA/CPTitle/isbn-0471241083.html |
Citation | Current Protocols in Cell Biology, 2017, v. 77, p. 19.20.1-19.20.10 How to Cite? |
Abstract | Previously, high-resolution three-dimensional imaging of a whole and intact pancreas was not possible, since light is scattered when it passes through cell compartments with different refractive indices. CLARITY is one of the tissue clearing techniques that has yielded success with the central nervous system. To preserve tissue integrity after delipidation, conventional protocols embed tissue in an acrylamide-based hydrogel, which involves the use of specialized equipment. Recently, we determined that the hydrogel-embedding step could be simplified and replaced by passive tissue fixation in 4% paraformaldehyde (PFA). The whole procedure is less time-consuming and less error-prone, and can be completed within a week, compared to conventional CLARITY protocols that may take weeks to complete. Here, the detailed stepwise procedures involved in the simplified CLARITY workflow are applied to the pancreas of wild-type and gene-knockout 6-week old mice expressing green fluorescent protein (GFP) under the mouse insulin 1 promoter (MIP-GFP). This technique could facilitate high-resolution, three-dimensional imaging of pancreatic islets and comparison between different mouse genotypes under different disease and treatment conditions. |
Persistent Identifier | http://hdl.handle.net/10722/261219 |
ISSN | 2023 SCImago Journal Rankings: 1.872 |
DC Field | Value | Language |
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dc.contributor.author | Wong, HS | - |
dc.contributor.author | Yeung, PKK | - |
dc.contributor.author | Lai, HM | - |
dc.contributor.author | Lam, KSL | - |
dc.contributor.author | Wu, W | - |
dc.contributor.author | Chung, SK | - |
dc.date.accessioned | 2018-09-14T08:54:30Z | - |
dc.date.available | 2018-09-14T08:54:30Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Current Protocols in Cell Biology, 2017, v. 77, p. 19.20.1-19.20.10 | - |
dc.identifier.issn | 1934-2500 | - |
dc.identifier.uri | http://hdl.handle.net/10722/261219 | - |
dc.description.abstract | Previously, high-resolution three-dimensional imaging of a whole and intact pancreas was not possible, since light is scattered when it passes through cell compartments with different refractive indices. CLARITY is one of the tissue clearing techniques that has yielded success with the central nervous system. To preserve tissue integrity after delipidation, conventional protocols embed tissue in an acrylamide-based hydrogel, which involves the use of specialized equipment. Recently, we determined that the hydrogel-embedding step could be simplified and replaced by passive tissue fixation in 4% paraformaldehyde (PFA). The whole procedure is less time-consuming and less error-prone, and can be completed within a week, compared to conventional CLARITY protocols that may take weeks to complete. Here, the detailed stepwise procedures involved in the simplified CLARITY workflow are applied to the pancreas of wild-type and gene-knockout 6-week old mice expressing green fluorescent protein (GFP) under the mouse insulin 1 promoter (MIP-GFP). This technique could facilitate high-resolution, three-dimensional imaging of pancreatic islets and comparison between different mouse genotypes under different disease and treatment conditions. | - |
dc.language | eng | - |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://cda.currentprotocols.com/WileyCDA/CPTitle/isbn-0471241083.html | - |
dc.relation.ispartof | Current Protocols in Cell Biology | - |
dc.rights | Current Protocols in Cell Biology. Copyright © John Wiley & Sons, Inc. | - |
dc.rights | Preprint: This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Postprint: This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Special Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.) | - |
dc.subject | CLARITY | - |
dc.subject | pancreas | - |
dc.subject | simple | - |
dc.subject | three-dimensional imaging | - |
dc.subject | tissue clearing | - |
dc.title | Simple and Rapid Tissue Clearing Method for Three-Dimensional Histology of the Pancreas | - |
dc.type | Article | - |
dc.identifier.email | Yeung, PKK: ykkp@hkucc.hku.hk | - |
dc.identifier.email | Lam, KSL: ksllam@hku.hk | - |
dc.identifier.email | Wu, W: wtwu@HKUCC-COM.hku.hk | - |
dc.identifier.email | Chung, SK: skchung@hkucc.hku.hk | - |
dc.identifier.authority | Lam, KSL=rp00343 | - |
dc.identifier.authority | Wu, W=rp00419 | - |
dc.identifier.authority | Chung, SK=rp00381 | - |
dc.identifier.doi | 10.1002/cpcb.34 | - |
dc.identifier.scopus | eid_2-s2.0-85049914557 | - |
dc.identifier.hkuros | 290670 | - |
dc.identifier.volume | 77 | - |
dc.identifier.spage | 19.20.1 | - |
dc.identifier.epage | 19.20.10 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1934-2616 | - |