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- Publisher Website: 10.1039/C7MT00275K
- Scopus: eid_2-s2.0-85041198758
- PMID: 29323384
- WOS: WOS:000423352400005
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Article: Tracking iron-associated proteomes in pathogens by a fluorescence approach
Title | Tracking iron-associated proteomes in pathogens by a fluorescence approach |
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Authors | |
Issue Date | 2018 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp |
Citation | Metallomics, 2018, v. 10 n. 1, p. 77-82 How to Cite? |
Abstract | Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials. |
Persistent Identifier | http://hdl.handle.net/10722/261362 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.706 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Jiang, N | - |
dc.contributor.author | Cheng, T | - |
dc.contributor.author | Wang, M | - |
dc.contributor.author | Chan, GCF | - |
dc.contributor.author | Jin, L | - |
dc.contributor.author | Li, H | - |
dc.contributor.author | Sun, H | - |
dc.date.accessioned | 2018-09-14T08:56:54Z | - |
dc.date.available | 2018-09-14T08:56:54Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Metallomics, 2018, v. 10 n. 1, p. 77-82 | - |
dc.identifier.issn | 1756-5901 | - |
dc.identifier.uri | http://hdl.handle.net/10722/261362 | - |
dc.description.abstract | Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp | - |
dc.relation.ispartof | Metallomics | - |
dc.title | Tracking iron-associated proteomes in pathogens by a fluorescence approach | - |
dc.type | Article | - |
dc.identifier.email | Cheng, T: chengtfc@hku.hk | - |
dc.identifier.email | Chan, GCF: gcfchan@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.email | Li, H: hylichem@hku.hk | - |
dc.identifier.email | Sun, H: hsun@hku.hk | - |
dc.identifier.authority | Chan, GCF=rp00431 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.identifier.authority | Sun, H=rp00777 | - |
dc.identifier.doi | 10.1039/C7MT00275K | - |
dc.identifier.pmid | 29323384 | - |
dc.identifier.scopus | eid_2-s2.0-85041198758 | - |
dc.identifier.hkuros | 291796 | - |
dc.identifier.hkuros | 292257 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 77 | - |
dc.identifier.epage | 82 | - |
dc.identifier.isi | WOS:000423352400005 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1756-5901 | - |