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- Publisher Website: 10.1242/dev.155838
- Scopus: eid_2-s2.0-85040777807
- PMID: 29217756
- WOS: WOS:000423820200018
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Article: Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro
Title | Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro |
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Authors | |
Keywords | Cataract Vx-770 Stem cell Organoid Lens development Focus |
Issue Date | 2018 |
Publisher | The Company of Biologists Ltd. The Journal's web site is located at https://dev.biologists.org/ |
Citation | Development (Cambridge), 2018, v. 145, n. 1, article no. dev155838 How to Cite? |
Abstract | © 2018. Published by The Company of Biologists Ltd. Cataracts cause vision loss and blindness by impairing the ability of the ocular lens to focus light onto the retina. Various cataract risk factors have been identified, including drug treatments, age, smoking and diabetes. However, the molecular events responsible for these different forms of cataract are ill-defined, and the advent of modern cataract surgery in the 1960s virtually eliminated access to human lenses for research. Here, we demonstrate large-scale production of light-focusing human micro-lenses from spheroidal masses of human lens epithelial cells purified from differentiating pluripotent stem cells. The purified lens cells and micro-lenses display similar morphology, cellular arrangement, mRNA expression and protein expression to human lens cells and lenses. Exposing the micro-lenses to the emergent cystic fibrosis drug Vx-770 reduces micro-lens transparency and focusing ability. These human micro-lenses provide a powerful and large-scale platform for defining molecular disease mechanisms caused by cataract risk factors, for anti-cataract drug screening and for clinically relevant toxicity assays. |
Persistent Identifier | http://hdl.handle.net/10722/262778 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 1.852 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Murphy, Patricia | - |
dc.contributor.author | Kabir, Md Humayun | - |
dc.contributor.author | Srivastava, Tarini | - |
dc.contributor.author | Mason, Michele E. | - |
dc.contributor.author | Dewi, Chitra U. | - |
dc.contributor.author | Lim, Seakcheng | - |
dc.contributor.author | Yang, Andrian | - |
dc.contributor.author | Djordjevic, Djordje | - |
dc.contributor.author | Killingsworth, Murray C. | - |
dc.contributor.author | Ho, Joshua W.K. | - |
dc.contributor.author | Harman, David G. | - |
dc.contributor.author | O’Connor, Michael D. | - |
dc.date.accessioned | 2018-10-08T02:47:00Z | - |
dc.date.available | 2018-10-08T02:47:00Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Development (Cambridge), 2018, v. 145, n. 1, article no. dev155838 | - |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | http://hdl.handle.net/10722/262778 | - |
dc.description.abstract | © 2018. Published by The Company of Biologists Ltd. Cataracts cause vision loss and blindness by impairing the ability of the ocular lens to focus light onto the retina. Various cataract risk factors have been identified, including drug treatments, age, smoking and diabetes. However, the molecular events responsible for these different forms of cataract are ill-defined, and the advent of modern cataract surgery in the 1960s virtually eliminated access to human lenses for research. Here, we demonstrate large-scale production of light-focusing human micro-lenses from spheroidal masses of human lens epithelial cells purified from differentiating pluripotent stem cells. The purified lens cells and micro-lenses display similar morphology, cellular arrangement, mRNA expression and protein expression to human lens cells and lenses. Exposing the micro-lenses to the emergent cystic fibrosis drug Vx-770 reduces micro-lens transparency and focusing ability. These human micro-lenses provide a powerful and large-scale platform for defining molecular disease mechanisms caused by cataract risk factors, for anti-cataract drug screening and for clinically relevant toxicity assays. | - |
dc.language | eng | - |
dc.publisher | The Company of Biologists Ltd. The Journal's web site is located at https://dev.biologists.org/ | - |
dc.relation.ispartof | Development (Cambridge) | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Cataract | - |
dc.subject | Vx-770 | - |
dc.subject | Stem cell | - |
dc.subject | Organoid | - |
dc.subject | Lens development | - |
dc.subject | Focus | - |
dc.title | Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1242/dev.155838 | - |
dc.identifier.pmid | 29217756 | - |
dc.identifier.scopus | eid_2-s2.0-85040777807 | - |
dc.identifier.volume | 145 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. dev155838 | - |
dc.identifier.epage | article no. dev155838 | - |
dc.identifier.eissn | 1477-9129 | - |
dc.identifier.isi | WOS:000423820200018 | - |
dc.identifier.f1000 | 732248600 | - |
dc.identifier.issnl | 0950-1991 | - |