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Book Chapter: Genome-Wide Mapping of Protein-DNA Interactions by ChIP-Seq

TitleGenome-Wide Mapping of Protein-DNA Interactions by ChIP-Seq
Authors
Ho, Joshua W.K.Alekseyenko, Artyom A.Kuroda, Mitzi I.Park, Peter J.
KeywordsProtein-DNA interactions
Genome-wide mapping
ChIP-Seq
Applications
Methods
Issue Date2012
Citation
Tag-Based Next Generation Sequencing, 2012, p. 139-151 How to Cite?
DOI: http://dx.doi.org/10.1002/9783527644582.ch9
AbstractChromatin immunoprecipitation (ChIP) followed by massively parallel sequencing (ChIP-seq) has enabled generation of genome-wide maps of various types of in vivo protein-DNA interaction at an unprecedented resolution. These maps have led to many important insights into the regulatory mechanisms of gene expression through transcription factor binding and epigenetic modifications. In this chapter, we present a common ChIP-seq experimental and analysis pipeline, and give examples of how ChIP-seq can be applied to tackle various biological questions. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/262822

 

DC FieldValueLanguage
dc.contributor.authorHo, Joshua W.K.-
dc.contributor.authorAlekseyenko, Artyom A.-
dc.contributor.authorKuroda, Mitzi I.-
dc.contributor.authorPark, Peter J.-
dc.date.accessioned2018-10-08T02:47:10Z-
dc.date.available2018-10-08T02:47:10Z-
dc.date.issued2012-
dc.identifier.citationTag-Based Next Generation Sequencing, 2012, p. 139-151-
dc.identifier.urihttp://hdl.handle.net/10722/262822-
dc.description.abstractChromatin immunoprecipitation (ChIP) followed by massively parallel sequencing (ChIP-seq) has enabled generation of genome-wide maps of various types of in vivo protein-DNA interaction at an unprecedented resolution. These maps have led to many important insights into the regulatory mechanisms of gene expression through transcription factor binding and epigenetic modifications. In this chapter, we present a common ChIP-seq experimental and analysis pipeline, and give examples of how ChIP-seq can be applied to tackle various biological questions. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.-
dc.languageeng-
dc.relation.ispartofTag-Based Next Generation Sequencing-
dc.subjectProtein-DNA interactions-
dc.subjectGenome-wide mapping-
dc.subjectChIP-Seq-
dc.subjectApplications-
dc.subjectMethods-
dc.titleGenome-Wide Mapping of Protein-DNA Interactions by ChIP-Seq-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/9783527644582.ch9-
dc.identifier.scopuseid_2-s2.0-84886030015-
dc.identifier.spage139-
dc.identifier.epage151-

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