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Article: NADPH oxidase 4 mediates reactive oxygen species induction of CD146 dimerization in VEGF signal transduction

TitleNADPH oxidase 4 mediates reactive oxygen species induction of CD146 dimerization in VEGF signal transduction
Authors
KeywordsVEGF signal transduction
Small GTPase
NADPH oxidase
Free radicals
CD146 dimerization
Issue Date2010
Citation
Free Radical Biology and Medicine, 2010, v. 49, n. 2, p. 227-236 How to Cite?
AbstractCD146 dimerization plays an important role in tumor-induced angiogenesis. Stimulation of target cells with vascular endothelial growth factor (VEGF), a major angiogenic factor produced by tumor cells, elicits a burst of reactive oxygen species (ROS) that enhances angiogenesis. However, the molecular mechanism coupling CD146 dimerization with the VEGF-related oxidant-generating apparatus has not been elucidated. Here, we show that CD146 dimerization is induced by VEGF and is significantly diminished by pretreatment with diphenylene iodonium, an inhibitor of NADPH oxidase, suggesting a potential role for NADPH oxidase (NOX) in VEGF-induced CD146 dimerization. Importantly, we found that overexpression of NADPH oxidase 4 (NOX4), which is the predominant NOX expressed in endothelial cells, significantly enhances VEGF-induced ROS generation and CD146 dimerization. By contrast, these VEGF effects were dramatically attenuated after transfection with siRNA to reduce NOX4 expression. Furthermore, expression of Rac1 N17, a dominant negative mutant of Rac1, a member of the Rho family of small GTPases, suppressed VEGF-induced ROS generation and CD146 dimerization. These studies show for the first time that VEGF alteration of CD146 dimerization is mediated via a NOX4-dependent pathway and provide novel insight into the significant role of NOX in redox regulation of the dimerization of cell adhesion molecules. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/265578
ISSN
2020 Impact Factor: 7.376
2020 SCImago Journal Rankings: 1.912
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhuang, Jie-
dc.contributor.authorJiang, Tianxia-
dc.contributor.authorLu, Di-
dc.contributor.authorLuo, Yongting-
dc.contributor.authorZheng, Chaogu-
dc.contributor.authorFeng, Jing-
dc.contributor.authorYang, Dongling-
dc.contributor.authorChen, Chang-
dc.contributor.authorYan, Xiyun-
dc.date.accessioned2018-12-03T01:21:04Z-
dc.date.available2018-12-03T01:21:04Z-
dc.date.issued2010-
dc.identifier.citationFree Radical Biology and Medicine, 2010, v. 49, n. 2, p. 227-236-
dc.identifier.issn0891-5849-
dc.identifier.urihttp://hdl.handle.net/10722/265578-
dc.description.abstractCD146 dimerization plays an important role in tumor-induced angiogenesis. Stimulation of target cells with vascular endothelial growth factor (VEGF), a major angiogenic factor produced by tumor cells, elicits a burst of reactive oxygen species (ROS) that enhances angiogenesis. However, the molecular mechanism coupling CD146 dimerization with the VEGF-related oxidant-generating apparatus has not been elucidated. Here, we show that CD146 dimerization is induced by VEGF and is significantly diminished by pretreatment with diphenylene iodonium, an inhibitor of NADPH oxidase, suggesting a potential role for NADPH oxidase (NOX) in VEGF-induced CD146 dimerization. Importantly, we found that overexpression of NADPH oxidase 4 (NOX4), which is the predominant NOX expressed in endothelial cells, significantly enhances VEGF-induced ROS generation and CD146 dimerization. By contrast, these VEGF effects were dramatically attenuated after transfection with siRNA to reduce NOX4 expression. Furthermore, expression of Rac1 N17, a dominant negative mutant of Rac1, a member of the Rho family of small GTPases, suppressed VEGF-induced ROS generation and CD146 dimerization. These studies show for the first time that VEGF alteration of CD146 dimerization is mediated via a NOX4-dependent pathway and provide novel insight into the significant role of NOX in redox regulation of the dimerization of cell adhesion molecules. © 2010 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofFree Radical Biology and Medicine-
dc.subjectVEGF signal transduction-
dc.subjectSmall GTPase-
dc.subjectNADPH oxidase-
dc.subjectFree radicals-
dc.subjectCD146 dimerization-
dc.titleNADPH oxidase 4 mediates reactive oxygen species induction of CD146 dimerization in VEGF signal transduction-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.freeradbiomed.2010.04.007-
dc.identifier.pmid20403426-
dc.identifier.scopuseid_2-s2.0-77953542905-
dc.identifier.volume49-
dc.identifier.issue2-
dc.identifier.spage227-
dc.identifier.epage236-
dc.identifier.isiWOS:000279017800012-
dc.identifier.issnl0891-5849-

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