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Article: Pea Ferritin Stability under Gastric pH Conditions Determines the Mechanism of Iron Uptake in Caco-2 Cells
Title | Pea Ferritin Stability under Gastric pH Conditions Determines the Mechanism of Iron Uptake in Caco-2 Cells |
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Authors | |
Keywords | Bioavailability DMT-1 Endocytosis Iron absorption Pea ferritin |
Issue Date | 2018 |
Publisher | Oxford University Press. The Journal's web site is located at http://jn.nutrition.org |
Citation | The Journal of Nutrition, 2018, v. 148 n. 8, p. 1229-1235 How to Cite? |
Abstract | Background: Iron deficiency is an enduring global health problem that requires new remedial approaches. Iron absorption from soybean-derived ferritin, an ∼550-kDa iron storage protein, is comparable to bioavailable ferrous sulfate (FeSO4). However, the absorption of ferritin is reported to involve an endocytic mechanism, independent of divalent metal ion transporter 1 (DMT-1), the transporter for nonheme iron. Objective: Our overall aim was to examine the potential of purified ferritin from peas (Pisum sativum) as a food supplement by measuring its stability under gastric pH treatment and the mechanisms of iron uptake into Caco-2 cells. Methods: Caco-2 cells were treated with native or gastric pH-treated pea ferritin in combination with dietary modulators of nonheme iron uptake, small interfering RNA targeting DMT-1, or chemical inhibitors of endocytosis. Cellular ferritin formation, a surrogate measure of iron uptake, and internalization of pea ferritin with the use of specific antibodies were measured. The production of reactive oxygen species (ROS) in response to equimolar concentrations of native pea ferritin and FeSO4 was also compared. Results: Pea ferritin exposed to gastric pH treatment was degraded, and the released iron was transported into Caco-2 cells by DMT-1. Inhibitors of DMT-1 and nonheme iron absorption reduced iron uptake by 26-40%. Conversely, in the absence of gastric pH treatment, the iron uptake of native pea ferritin was unaffected by inhibitors of nonheme iron absorption, and the protein was observed to be internalized in Caco-2 cells. Chlorpromazine (clathrin-mediated endocytosis inhibitor) reduced the native pea ferritin content within cells by ∼30%, which confirmed that the native pea ferritin was transported into cells via a clathrin-mediated endocytic pathway. In addition, 60% less ROS production resulted from native pea ferritin in comparison to FeSO4. Conclusion: With consideration that nonheme dietary inhibitors display no effect on iron uptake and the low oxidative potential relative to FeSO4, intact pea ferritin appears to be a promising iron supplement. © 2018 American Society for Nutrition. |
Persistent Identifier | http://hdl.handle.net/10722/266544 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 1.098 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Perfecto, AP | - |
dc.contributor.author | Rodriguez-Ramiro, IRR | - |
dc.contributor.author | Rodriguez-Celma, JRC | - |
dc.contributor.author | Sharp, PS | - |
dc.contributor.author | Balk, JB | - |
dc.contributor.author | Fairweather-Tait, SFT | - |
dc.date.accessioned | 2019-01-21T01:41:29Z | - |
dc.date.available | 2019-01-21T01:41:29Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | The Journal of Nutrition, 2018, v. 148 n. 8, p. 1229-1235 | - |
dc.identifier.issn | 0022-3166 | - |
dc.identifier.uri | http://hdl.handle.net/10722/266544 | - |
dc.description.abstract | Background: Iron deficiency is an enduring global health problem that requires new remedial approaches. Iron absorption from soybean-derived ferritin, an ∼550-kDa iron storage protein, is comparable to bioavailable ferrous sulfate (FeSO4). However, the absorption of ferritin is reported to involve an endocytic mechanism, independent of divalent metal ion transporter 1 (DMT-1), the transporter for nonheme iron. Objective: Our overall aim was to examine the potential of purified ferritin from peas (Pisum sativum) as a food supplement by measuring its stability under gastric pH treatment and the mechanisms of iron uptake into Caco-2 cells. Methods: Caco-2 cells were treated with native or gastric pH-treated pea ferritin in combination with dietary modulators of nonheme iron uptake, small interfering RNA targeting DMT-1, or chemical inhibitors of endocytosis. Cellular ferritin formation, a surrogate measure of iron uptake, and internalization of pea ferritin with the use of specific antibodies were measured. The production of reactive oxygen species (ROS) in response to equimolar concentrations of native pea ferritin and FeSO4 was also compared. Results: Pea ferritin exposed to gastric pH treatment was degraded, and the released iron was transported into Caco-2 cells by DMT-1. Inhibitors of DMT-1 and nonheme iron absorption reduced iron uptake by 26-40%. Conversely, in the absence of gastric pH treatment, the iron uptake of native pea ferritin was unaffected by inhibitors of nonheme iron absorption, and the protein was observed to be internalized in Caco-2 cells. Chlorpromazine (clathrin-mediated endocytosis inhibitor) reduced the native pea ferritin content within cells by ∼30%, which confirmed that the native pea ferritin was transported into cells via a clathrin-mediated endocytic pathway. In addition, 60% less ROS production resulted from native pea ferritin in comparison to FeSO4. Conclusion: With consideration that nonheme dietary inhibitors display no effect on iron uptake and the low oxidative potential relative to FeSO4, intact pea ferritin appears to be a promising iron supplement. © 2018 American Society for Nutrition. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press. The Journal's web site is located at http://jn.nutrition.org | - |
dc.relation.ispartof | The Journal of Nutrition | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Bioavailability | - |
dc.subject | DMT-1 | - |
dc.subject | Endocytosis | - |
dc.subject | Iron absorption | - |
dc.subject | Pea ferritin | - |
dc.title | Pea Ferritin Stability under Gastric pH Conditions Determines the Mechanism of Iron Uptake in Caco-2 Cells | - |
dc.type | Article | - |
dc.identifier.email | Perfecto, AP: perfecto@hku.hk | - |
dc.identifier.authority | Perfecto, AP=rp02514 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1093/jn/nxy096 | - |
dc.identifier.pmid | 29939292 | - |
dc.identifier.scopus | eid_2-s2.0-85055537879 | - |
dc.identifier.hkuros | 296681 | - |
dc.identifier.volume | 148 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 1229 | - |
dc.identifier.epage | 1235 | - |
dc.identifier.isi | WOS:000441347500004 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-3166 | - |